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Cellosaurus CHO-DG44 (CVCL_7180)

[Text version]
Cell line name CHO-DG44
Synonyms CHO DG44; CHO-DG44 (DHFR-); CHO DG-44; DG-44; DG44
Accession CVCL_7180
Resource Identification Initiative To cite this cell line use: CHO-DG44 (RRID:CVCL_7180)
Comments Characteristics: Lacks both copies of the Dhfr gene.
Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Omics: Deep proteome analysis.
Omics: Genome sequenced.
Derived from sampling site: Ovary.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7182 (CHO Pro-3)
CVCL_A4AR (Agarabi CHO)CVCL_VU03 (CHO B8-300)CVCL_VU65 (CHO E1a)
CVCL_KA66 (CHO-DG44 (cGMP banked))CVCL_B0VY (CHO/CERT 2.20)CVCL_B0VZ (CHO/CERT 2.41)
CVCL_YY02 (CHOmt17-0)CVCL_WB19 (CHOpper Discovery)CVCL_WB20 (CHOpper Standard)
CVCL_5A35 (haAQC2)CVCL_5A34 (hAQC2)CVCL_5A36 (hsAQC2)
CVCL_KA70 (Ms704)CVCL_KA71 (MS705)CVCL_KA72 (Ms709)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Chinese_hamster_ovary_cell

PubMed=6305508; DOI=10.1016/0092-8674(83)90422-1
Urlaub G., Kas E., Carothers A.M., Chasin L.A.
Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells.
Cell 33:405-412(1983)

PubMed=16403443; DOI=10.1016/j.bbrc.2005.12.111
Derouazi M., Martinet D., Besuchet-Schmutz N., Flaction R., Wicht M., Bertschinger M., Hacker D.L., Beckmann J.S., Wurm F.M.
Genetic characterization of CHO production host DG44 and derivative recombinant cell lines.
Biochem. Biophys. Res. Commun. 340:1069-1077(2006)

Martinet D., Derouazi M., Besuchet-Schmutz N., Wicht M., Beckmann J.S., Wurm F.M.
Karyotype of CHO DG44 cells.
(In) Cell technology for cell products; Smith R. (eds.); pp.363-366; Springer; Dordrecht (2007)

PubMed=21345355; DOI=10.1016/j.jbiotec.2011.02.002
Florin L., Lipske C., Becker E., Kaufmann H.
Supplementation of serum free media with HT is not sufficient to restore growth properties of DHFR-/- cells in fed-batch processes -- implications for designing novel CHO-based expression platforms.
J. Biotechnol. 152:189-193(2011)

Wurm F.M.
CHO quasispecies -- implications for manufacturing processes.
Processes 1:296-311(2013)

PubMed=23873082; DOI=10.1038/nbt.2624
Lewis N.E., Liu X., Li Y.-X., Nagarajan H., Yerganian G., O'Brien E., Bordbar A., Roth A.M., Rosenbloom J., Bian C., Xie M., Chen W.-B., Li N., Baycin-Hizal D., Latif H., Forster J., Betenbaugh M.J., Famili I., Xu X., Wang J., Palsson B.O.
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.
Nat. Biotechnol. 31:759-765(2013)

Wurm F.M., Wurm M.J.
Cloning of CHO cells, productivity and genetic stability -- a discussion.
Processes 5:20.1-20.13(2017)

PubMed=28876938; DOI=10.1021/acs.jproteome.7b00382
Heffner K.M., Hizal D.B., Yerganian G., Kumar A., Can O., O'Meally R., Cole R., Chaerkady R., Wu H., Bowen M.A., Betenbaugh M.J.
Lessons from the hamster: Cricetulus griseus tissue and CHO cell line proteome comparison.
J. Proteome Res. 16:3672-3687(2017)

PubMed=29987698; DOI=10.1007/s10616-018-0233-5
Kimura S., Omasa T.
Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and mouse using end sequences of CHO BAC clones based on BAC-FISH results.
Cytotechnology 70:1399-1407(2018)

PubMed=32619503; DOI=10.1016/j.ymben.2020.06.002
Szeliova D., Ruckerbauer D.E., Galleguillos S.N., Petersen L.B., Natter K., Hanscho M., Troyer C., Causon T., Schoeny H., Christensen H.B., Lee D.-Y., Lewis N.E., Koellensperger G., Hann S., Nielsen L.K., Borth N., Zanghellini J.
What CHO is made of: variations in the biomass composition of Chinese hamster ovary cell lines.
Metab. Eng. 61:288-300(2020)

PubMed=32985598; DOI=10.1038/s41598-020-72959-8
Heffner K.M., Hizal D.B., Majewska N.I., Kumar S., Dhara V.G., Zhu J., Bowen M.A., Hatton D., Yerganian G., Yerganian A., O'Meally R., Cole R., Betenbaugh M.J.
Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities.
Sci. Rep. 10:15841-15841(2020)

PubMed=34050613; DOI=10.1002/biot.202100165
Wurm M.J., Wurm F.M.
Naming CHO cells for bio-manufacturing: genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names.
Biotechnol. J. 16:e2100165.1-e2100165.13(2021)

Cell line collections NCBI_Iran; C576
Cell line databases/resources Lonza; 467
Lonza; 468
TOKU-E; 3678
Encyclopedic resources Wikidata; Q54812671
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number24