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Cellosaurus CHOpper Discovery (CVCL_WB19)

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Cell line name CHOpper Discovery
Accession CVCL_WB19
Resource Identification Initiative To cite this cell line use: CHOpper Discovery (RRID:CVCL_WB19)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ); ACC-2909.
Doubling time: ~17 hours (Patent=US8962312).
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=UniProtKB; Q2MH30; Cricetulus griseus Dhfr.
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=21345355; DOI=10.1016/j.jbiotec.2011.02.002
Lore Florin, Carolin Lipske, Eric Becker, Hitto Kaufmann;
Supplementation of serum free media with HT is not sufficient to restore growth properties of DHFR-/- cells in fed-batch processes -- implications for designing novel CHO-based expression platforms.
J. Biotechnol. 152:189-193(2011)

Patent=US8962312
Hitto Kaufmann, Lore Florin, Eric Becker, Joey M. Studts;
Production host cell lines.
Patent number US8962312, 24-Feb-2015

Cross-references
Encyclopedic resources Wikidata; Q93453047
Entry history
Entry creation24-May-2019
Last entry update19-Dec-2024
Version number9