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Cellosaurus CHO/ASCT2 (CVCL_D3PI)

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Cell line name CHO/ASCT2
Synonyms ASCT2/CHO
Accession CVCL_D3PI
Resource Identification Initiative To cite this cell line use: CHO/ASCT2 (RRID:CVCL_D3PI)
Comments Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:10943; SLC1A5 (Note=With a C-terminal mycHis6 tag).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=27865832; DOI=10.1016/j.bbrc.2016.11.089
Masayo Suzuki, Hiroe Toki, Akiko Furuya, Hiroshi Ando;
Establishment of monoclonal antibodies against cell surface domains of ASCT2/SLC1A5 and their inhibition of glutamine-dependent tumor cell growth.
Biochem. Biophys. Res. Commun. 482:651-657(2017)

Cross-references
Encyclopedic resources Wikidata; Q127380485
Entry history
Entry creation30-Jan-2024
Last entry update14-Aug-2025
Version number4