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Cellosaurus CHO/CERT 2.20 (CVCL_B0VY)

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Cell line name CHO/CERT 2.20
Accession CVCL_B0VY
Resource Identification Initiative To cite this cell line use: CHO/CERT 2.20 (RRID:CVCL_B0VY)
Comments Group: Patented cell line.
Registration: International Depositary Authority, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ); ACC-2989.
Characteristics: Has improved secretion characteristics in comparison to non-transgenic cell lines.
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:2205; CERT1 (Note=With p.Ser132Ala).
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=19428735; DOI=10.1016/j.jbiotec.2009.02.014
Lore Florin, Antje Pegel, Eric Becker, Angelika Hausser, Monilola Afolabi Olayioye, Hitto Kaufmann;
Heterologous expression of the lipid transfer protein CERT increases therapeutic protein productivity of mammalian cells.
J. Biotechnol. 141:84-90(2009)

Patent=US9340592
Lore Florin, Eric Becker, Hitto Kaufmann;
CHO/CERT cell lines.
Patent number US9340592, 17-May-2016

Cross-references
Encyclopedic resources Wikidata; Q108820040
Entry history
Entry creation23-Sep-2021
Last entry update14-Aug-2025
Version number8