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Cellosaurus CHO B8-300 (CVCL_VU03)

[Text version]
Cell line name CHO B8-300
Synonyms B8-300
Accession CVCL_VU03
Resource Identification Initiative To cite this cell line use: CHO B8-300 (RRID:CVCL_VU03)
Comments Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3415; EPO.
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7180 (CHO-DG44)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=3865178; DOI=10.1073/pnas.82.22.7580; PMCID=PMC391376
Fu-Kuen Lin, Sidney V. Suggs, Chi-Hwei Lin, Jeffrey K. Browne, Ralph Smalling, Joan C. Egrie, Kenneth K. Chen, Gary M. Fox, Frank Martin, Zippora Stabinsky, Sayed M. Badrawi ...Show all 13 authors... , Por-Hsiung Lai, Eugene Goldwasser; Show fewer authors
Cloning and expression of the human erythropoietin gene.
Proc. Natl. Acad. Sci. U.S.A. 82:7580-7584(1985)

PubMed=2813359; DOI=10.1073/pnas.86.20.7819; PMCID=PMC298162
Makoto Takeuchi, Noboru Inoue, Thomas W. Strickland, Mamoru Kubota, Michihito Wada, Rie Shimizu, Sakuo Hoshi, Hiroyuki Kozutsumi, Seiichi Takasaki, Akira Kobata;
Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells.
Proc. Natl. Acad. Sci. U.S.A. 86:7819-7822(1989)

Cross-references
Encyclopedic resources Wikidata; Q93451114
Entry history
Entry creation24-May-2019
Last entry update19-Dec-2024
Version number8