ID   CHO B8-300
AC   CVCL_VU03
SY   B8-300
DR   Wikidata; Q93451114
RX   PubMed=2813359;
RX   PubMed=3865178;
CC   Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
CC   Sequence variation: Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:3415; EPO.
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
CC   Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_7180 ! CHO-DG44
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 24-05-19; Last updated: 19-12-24; Version: 8
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RX   PubMed=2813359; DOI=10.1073/pnas.86.20.7819; PMCID=PMC298162;
RA   Takeuchi M., Inoue N., Strickland T.W., Kubota M., Wada M.,
RA   Shimizu R., Hoshi S., Kozutsumi H., Takasaki S., Kobata A.;
RT   "Relationship between sugar chain structure and biological activity of
RT   recombinant human erythropoietin produced in Chinese hamster ovary
RT   cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:7819-7822(1989).
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RX   PubMed=3865178; DOI=10.1073/pnas.82.22.7580; PMCID=PMC391376;
RA   Lin F.-K., Suggs S.V., Lin C.-H., Browne J.K., Smalling R., Egrie J.C.,
RA   Chen K.K., Fox G.M., Martin F., Stabinsky Z., Badrawi S.M., Lai P.-H.,
RA   Goldwasser E.;
RT   "Cloning and expression of the human erythropoietin gene.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:7580-7584(1985).
//