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Cellosaurus KY821 (CVCL_1346)

[Text version]
Cell line name KY821
Synonyms KY-821
Accession CVCL_1346
Resource Identification Initiative To cite this cell line use: KY821 (RRID:CVCL_1346)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Doubling time: ~2 days (Note=Lot 11022006) (JCRB=JCRB0105).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; PTM; Phosphorylation; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Brain, meninges; UBERON=UBERON_0010743.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61His (c.183A>C); ClinVar=VCV000375871; Zygosity=Heterozygous (Cosmic-CLP=907300; DepMap=ACH-002262).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Homozygous (PubMed=1571549; Cosmic-CLP=907300; DepMap=ACH-002262).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North76.02
East Asian, South23.98
South Asian0
European, North0
European, South0
Disease Adult acute myelomonocytic leukemia (NCIt: C7962)
Acute myelomonocytic leukemia (ORDO: Orphanet_517)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_Z940 (KY-ADR2)CVCL_W293 (KY-MTX)CVCL_W294 (KY-Ra)
CVCL_5A05 (KY-Rb)CVCL_W295 (KY-VCR)CVCL_2806 (KY821A3)
Sex of cell Male
Age at sampling 28Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=907300; JCRB=JCRB0105

Markers:
AmelogeninX
CSF1PO10,12
D5S81810,13
D7S82012
D13S3179,12
D16S53910,12
TH019
TPOX11
vWA17,18

Run an STR similarity search on this cell line
Publications

CLPUB00069
Kenji Kishi, Masaaki Kano, Hideo Hirosawa, Tadashi Koike, Chikara Sakai, Noritaka Aoyagi, Masayoshi Sanada, Yoshiaki Moriyama, Akira Hattori, Akira Shibata;
On the characteristics of a cell line (KY821) derived from acute myelogenous leukemia.
Acta Haematol. Jpn. 46:122.109-122.109(1983)

PubMed=1560671; DOI=10.1016/0145-2126(92)90059-G
Hiroyuki Saito, Kenji Kishi, Miwako Narita, Tatsuo Furukawa, Ei-ichi Nagura, Taira Maekawa, Tatsuo Abe, Akira Shibata;
A new myeloblastic leukemia cell line with double minute chromosomes Induction of methotrexate resistance and dihydrofolate reductase gene amplification.
Leuk. Res. 16:217-226(1992)

PubMed=1571549; DOI=10.1182/blood.V79.9.2378.2378
Koichi Sugimoto, Hideo Toyoshima, Ryuichi Sakai, Kiyoshi Miyagawa, Koichi Hagiwara, Fuyuki Ishikawa, Fumimaro Takaku, Yoshio Yazaki, Hisamaru Hirai;
Frequent mutations in the p53 gene in human myeloid leukemia cell lines.
Blood 79:2378-2383(1992)

PubMed=8387619; DOI=10.1016/0145-2126(93)90019-H
Takeaki Fukuda, Toshio Kakihara, Tomoko Kamishima, Yoshihisa Ohnishi, Makoto Naito, Kenji Kishi, Akira Shibata;
Different effects of various hematopoietic growth factors on myelomonocytic cell line (KY-821) and its drug-resistant sublines.
Leuk. Res. 17:325-332(1993)

PubMed=9747033; DOI=10.1007/s100380050067
Yoji Ariyama, Tomoya Sakabe, Takashi Shinomiya, Toshiki Mori, Yoji Fukuda, Johji Inazawa;
Identification of amplified DNA sequences on double minute chromosomes in a leukemic cell line KY821 by means of spectral karyotyping and comparative genomic hybridization.
J. Hum. Genet. 43:187-190(1998)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21552520; DOI=10.1371/journal.pone.0019169; PMCID=PMC3084268
Ting-Lei Gu, Julie Nardone, Yi Wang, Marc Loriaux, Judit Villen, Sean A. Beausoleil, Meghan Tucker, Jon M. Kornhauser, Jian-Min Ren, Joan MacNeill, Steven P. Gygi ...Show all 15 authors... , Brian Jay Druker, Michael Charles Heinrich, John Rush, Roberto D. Polakiewicz; Show fewer authors
Survey of activated FLT3 signaling in leukemia.
PLoS ONE 6:e19169.1-e19169.10(2011)

PubMed=23955599; DOI=10.1038/ng.2731
Ayana Kon, Lee-Yung Shih, Masashi Minamino, Masashi Sanada, Yuichi Shiraishi, Yasunobu Nagata, Ken-ichi Yoshida, Yusuke Okuno, Masashige Bando, Ryuichiro Nakato, Shumpei Ishikawa ...Show all 41 authors... , Aiko Sato-Otsubo, Genta Nagae, Aiko Nishimoto, Claudia Haferlach, Daniel Nowak, Yusuke Sato, Tamara Alpermann, Masao Nagasaki, Teppei Shimamura, Hiroko Tanaka, Kenichi Chiba, Ryo Yamamoto, Tomoyuki Yamaguchi, Makoto Otsu, Naoshi Obara, Mamiko Sakata-Yanagimoto, Tsuyoshi Nakamaki, Ken Ishiyama, Florian Nolte, Wolf-Karsten Hofmann, Shuichi Miyawaki, Shigeru Chiba, Hiraku Mori, Hiromitsu Nakauchi, Harold Phillip Koeffler, Hiroyuki Aburatani, Torsten Haferlach, Katsuhiko Shirahige, Satoru Miyano, Seishi Ogawa; Show fewer authors
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Nat. Genet. 45:1232-1237(2013)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB0105
Cell line databases/resources CLO; CLO_0009844
cancercelllines; CVCL_1346
Cell_Model_Passport; SIDM00553
Cosmic-CLP; 907300
DepMap; ACH-002262
LINCS_LDP; LCL-1004
Biological sample resources BioSample; SAMN03470792
Chemistry resources ChEMBL-Cells; CHEMBL3308834
ChEMBL-Targets; CHEMBL2366190
GDSC; 907300
PharmacoDB; KY821_797_2019
PubChem_Cell_line; CVCL_1346
Encyclopedic resources Wikidata; Q54900774
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM827265
GEO; GSM1670013
Polymorphism and mutation databases Cosmic; 907300
Cosmic; 975264
Cosmic; 2089651
Cosmic; 2131567
IARC_TP53; 2196
IARC_TP53; 21447
Progenetix; CVCL_1346
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number40