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Cellosaurus MOLT-3 (CVCL_0624)

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Cell line name MOLT-3
Synonyms Molt-3; MOLT 3; Molt 3; MOLT3; Molt3
Accession CVCL_0624
Resource Identification Initiative To cite this cell line use: MOLT-3 (RRID:CVCL_0624)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Doubling time: 24-48 hours (CLS=300116); ~40 hours (DSMZ=ACC-84).
Karyotypic information: Hypertetraploid karyotype (ATCC=CRL-1552).
Microsatellite instability: Instable (MSI) (PubMed=10739008; PubMed=31068700).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Leu1600Pro (c.4799T>C); Zygosity=Heterozygous (PubMed=15472075; DepMap=ACH-000964).
  • Mutation; HGNC; 7881; NOTCH1; Simple; p.Pro2514Argfs*4 (c.7541_7542delCT); ClinVar=VCV000581973; Zygosity=Heterozygous (PubMed=15472075; DepMap=ACH-000964).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (DepMap=ACH-000964).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA); ClinVar=VCV000092828; Zygosity=Homozygous (DepMap=ACH-000964).
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North2.31
East Asian, South0
South Asian0.44
European, North66.02
European, South30.32
Disease Adult T acute lymphoblastic leukemia (NCIt: C9142)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_S660 (MOLT-3/MTX.P-9)CVCL_S658 (MOLT-3/MTX10000)CVCL_S659 (MOLT-3/MTX200)
CVCL_S652 (MOLT-3/MTXt)CVCL_S653 (MOLT-3/TMQ200)CVCL_S655 (MOLT-3/TMQ2500)
CVCL_S656 (MOLT-3/TMQ800)CVCL_M774 (MOLT-3/Z29)CVCL_S661 (MOLT-3/ZD1694.C)
CVCL_S662 (MOLT-3/ZD1694.P9)CVCL_N031 (MOVP-3)
Originate from same individual CVCL_0013 ! MOLT-4
Sex of cell Male
Age at sampling 19Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-1552; CLS=300116; COG; DSMZ=ACC-84; PubMed=25877200; RCB=RCB1164

CSF1PO11,12 (ATCC=CRL-1552; COG; DSMZ=ACC-84)
11,12,13 (CLS=300116; PubMed=25877200; RCB=RCB1164)
D2S133822,23,24 (DSMZ=ACC-84)
23,24 (ATCC=CRL-1552; CLS=300116; COG)
D3S135814,15,16,17 (COG)
15,16,17 (ATCC=CRL-1552; CLS=300116; DSMZ=ACC-84; PubMed=25877200)
D5S81812 (ATCC=CRL-1552; PubMed=25877200)
12,13 (CLS=300116; DSMZ=ACC-84; RCB=RCB1164)
D7S8207,8,9 (RCB=RCB1164)
7,8,9,10 (CLS=300116; DSMZ=ACC-84)
8,10 (ATCC=CRL-1552; COG; PubMed=25877200)
D8S11799,13,14,15 (ATCC=CRL-1552; CLS=300116; PubMed=25877200)
9,14 (DSMZ=ACC-84)
9,14,15 (COG)
D13S31711,12,13 (RCB=RCB1164)
12,13 (ATCC=CRL-1552; CLS=300116; COG; DSMZ=ACC-84; PubMed=25877200)
D16S53910,11,13 (RCB=RCB1164)
11,12,14 (COG)
11,13,14 (ATCC=CRL-1552; PubMed=25877200)
11,14 (DSMZ=ACC-84)
11,14,15 (CLS=300116)
D18S5112,13,16,17 (CLS=300116; DSMZ=ACC-84)
13,17 (COG)
13,17,18 (ATCC=CRL-1552; PubMed=25877200)
D19S43314,15 (ATCC=CRL-1552; COG)
14,15,16 (CLS=300116; DSMZ=ACC-84)
D21S1128,29,30,31 (DSMZ=ACC-84)
29,30 (ATCC=CRL-1552; PubMed=25877200)
29,30,31,32 (CLS=300116)
FGA19,21,25 (CLS=300116)
21,22,23,24 (PubMed=25877200)
22,23,24 (DSMZ=ACC-84)
22,24 (ATCC=CRL-1552; COG)
Penta D8,13
Penta E14,16
vWA17 (ATCC=CRL-1552; COG; PubMed=25877200)
17,18 (CLS=300116; DSMZ=ACC-84; RCB=RCB1164)

Run an STR similarity search on this cell line
Web pages http://www.nibsc.org/Default.aspx?pageid=597&id=0010&theme=default

PubMed=4567231; DOI=10.1093/jnci/49.3.891
Minowada J., Onuma T., Moore G.E.
Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.
J. Natl. Cancer Inst. 49:891-895(1972)

PubMed=4547172; DOI=10.1093/jnci/53.3.655
Huang C.C., Hou Y., Woods L.K., Moore G.E., Minowada J.
Cytogenetic study of human lymphoid T-cell lines derived from lymphocytic leukemia.
J. Natl. Cancer Inst. 53:655-660(1974)

Kaplan J., Peterson W.D. Jr.
Detection of T-cell lymphoma-associated antigens on cord blood lymphocytes and phytohemagglutinin-stimulated blasts.
Cancer Res. 36:3471-3475(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=6600440; DOI=10.1007/BF02617996
Uittenbogaart C.H., Cantor Y., Fahey J.L.
Growth of human malignant lymphoid cell lines in serum-free medium.
In Vitro 19:67-72(1983)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C.
Leuk. Res. 9:549-559(1985)

Ohnuma T., Lo R.J., Scanlon K.J., Kamen B.A., Ohnoshi T., Wolman S.R., Holland J.F.
Evolution of methotrexate resistance of human acute lymphoblastic leukemia cells in vitro.
Cancer Res. 45:1815-1822(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2846092; DOI=10.1182/blood.V72.5.1755.1755
Greenberg J.M., Gonzalez-Sarmiento R., Arthur D.C., Wilkowski C.W., Streifel B.J., Kersey J.H.
Immunophenotypic and cytogenetic analysis of Molt-3 and Molt-4: human T-lymphoid cell lines with rearrangement of chromosome 7.
Blood 72:1755-1760(1988)

Zhou M.-X., Gu L.-B., James C.D., He J., Yeager A.M., Smith S.D., Findley H.W.
Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia.
Leukemia 9:1159-1161(1995)

PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x
Siebert R., Willers C.P., Schramm A., Fossa A., Dresen I.M.G., Uppenkamp M., Nowrousian M.R., Seeber S., Opalka B.
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Br. J. Haematol. 91:350-354(1995)

PubMed=8608962; DOI=10.1002/(SICI)1097-0215(19960328)66:1<29::AID-IJC6>3.0.CO;2-0
Takemura Y., Kobayashi H., Gibson W., Kimbell R., Miyachi H., Jackman A.L.
The influence of drug-exposure conditions on the development of resistance to methotrexate or ZD1694 in cultured human leukaemia cells.
Int. J. Cancer 66:29-36(1996)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=9787181; DOI=10.1182/blood.V92.9.3410
Sakai A., Thieblemont C., Wellmann A., Jaffe E.S., Raffeld M.
PTEN gene alterations in lymphoid neoplasms.
Blood 92:3410-3415(1998)

PubMed=9933131; DOI=10.1016/S0145-2126(98)00133-7
Burger R., Hansen-Hagge T.E., Drexler H.G., Gramatzki M.
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: suggestion for classification by immunophenotype and T-cell receptor studies.
Leuk. Res. 23:19-27(1999)

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Leuk. Res. 24:255-262(2000)

Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=11986953; DOI=10.1038/sj.leu.2402485
O'Donnell P.H., Guo W.-X., Reynolds C.P., Maurer B.J.
N-(4-hydroxyphenyl)retinamide increases ceramide and is cytotoxic to acute lymphoblastic leukemia cell lines, but not to non-malignant lymphocytes.
Leukemia 16:902-910(2002)

PubMed=12623853; DOI=10.1182/blood-2002-07-2268
Schroeder U., Bernt K.M., Lange B., Wenkel J., Jikai J., Shabat D., Amir R., Huebener N., Niethammer A.G., Hagemeier C., Wiebusch L., Gaedicke G., Wrasidlo W., Reisfeld R.A., Lode H.N.
Hydrolytically activated etoposide prodrugs inhibit MDR-1 function and eradicate established MDR-1 multidrug-resistant T-cell leukemia.
Blood 102:246-253(2003)

PubMed=15472075; DOI=10.1126/science.1102160
Weng A.P., Ferrando A.A., Lee W., Morris J.P. IV, Silverman L.B., Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=17170727; DOI=10.1038/sj.leu.2404486
Sandberg Y., Verhaaf B., van Gastel-Mol E.J., Wolvers-Tettero I.L.M., De Vos J., MacLeod R.A.F., Noordzij J.G., Dik W.A., van Dongen J.J.M., Langerak A.W.
Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.
Leukemia 21:230-237(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30629668; DOI=10.1371/journal.pone.0210404
Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.
Screening human cell lines for viral infections applying RNA-Seq data analysis.
PLoS ONE 14:E0210404-E0210404(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35354797; DOI=10.1038/s41467-022-29224-5
Leo I.R., Aswad L., Stahl M., Kunold E., Post F., Erkers T., Struyf N., Mermelekas G., Joshi R.N., Gracia-Villacampa E., Ostling P., Kallioniemi O.-P., Pokrovskaja Tamm K., Siavelis I., Lehtio J., Vesterlund M., Jafari R.
Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines.
Nat. Commun. 13:1691.1-1691.19(2022)

Cell line collections (Providers) ATCC; CRL-1552
BCRC; 60106
CLS; 300116
ECACC; 90021901
HIVReagentProgram; ARP-12187
RCB; RCB1164
Cell line databases/resources CLO; CLO_0007824
CLO; CLO_0051008
CLDB; cl3519
CLDB; cl3521
CLDB; cl3522
CLDB; cl3523
cancercelllines; CVCL_0624
Cell_Model_Passport; SIDM00153
DepMap; ACH-000964
DSMZCellDive; ACC-84
Anatomy/cell type resources BTO; BTO:0001081
Biological sample resources BioSample; SAMN03470846
BioSample; SAMN03471394
BioSample; SAMN03472164
BioSample; SAMN03473404
BioSample; SAMN10989586
Chemistry resources ChEMBL-Cells; CHEMBL3307556
ChEMBL-Targets; CHEMBL614176
PharmacoDB; MOLT3_957_2019
PubChem_Cell_line; CVCL_0624
Encyclopedic resources Wikidata; Q54906351
Experimental variables resources EFO; EFO_0005265
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
GEO; GSM236793
GEO; GSM236829
GEO; GSM5137730
Polymorphism and mutation databases Cosmic; 724815
Cosmic; 850193
Cosmic; 913410
Cosmic; 999756
Cosmic; 1191701
Cosmic; 1281377
Cosmic; 1524845
Cosmic; 2301561
Cosmic; 2361367
Cosmic; 2602923
LiGeA; CCLE_717
Progenetix; CVCL_0624
Proteomic databases PRIDE; PXD023662
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number34