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Cellosaurus MRC-5 (CVCL_0440)

[Text version]
Cell line name MRC-5
Synonyms MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5
Accession CVCL_0440
Resource Identification Initiative To cite this cell line use: MRC-5 (RRID:CVCL_0440)
Comments Group: Vaccine production cell line.
Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies).
Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta.
Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax).
Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax).
Senescence: Capable of attaining 42-46 population doublings before the onset of senescence (ATCC=CCL-171). Senesces at 26 PDL (PubMed=17395773).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 45 hours (Note=Lot 08072009), ~40 hours (Note=Lot 02272015) (JCRB=IFO50073); 36 hours (Note=Lot 041392), ~5 days (Note=Lot 031098), ~2 days (Note=Lot 05192003), ~1.5 days (Note=Lot 05202005), ~34 hours (Note=Lot 06022011), ~38 hours (Note=Lot 04212016) (JCRB=JCRB9008).
Omics: Genomics; DNA methylation analysis.
Omics: Glycomics; Glycosphingolipids.
Omics: Proteomics; 2DE-MS.
Omics: Proteomics; 2DE.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Misspelling: MCR-5; Note=Occasionally.
Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
Cell type: Fibroblast of lung; CL=CL_0002553.
HLA typing Source: PubMed=31869419
Class I
HLA-AA*02:01,29:02
HLA-BB*07:02,44:02
HLA-CC*05:01,07:02

Source: CLS=300395
Class I
HLA-AA*02:01:01,29:02:01
HLA-BB*07:02:01,44:02:01
HLA-CC*05:01:01,07:02:01
Class II
HLA-DPDPB1*04:01:01
HLA-DQDQA1*01:02:01,03:03:01
DQB1*03:01:01,06:02:01
HLA-DRDRB1*04:08:01,15:01:01G
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_2264 (1221)CVCL_2837 (A9(Neo1))CVCL_2838 (A9(Neo11))
CVCL_2844 (A9(Neo19))CVCL_2846 (A9(Neo20))CVCL_2848 (A9(Neo4))
CVCL_WT86 (AG27415)CVCL_F664 (Flow13000)CVCL_JG29 (HuS-L12/10)
CVCL_4W54 (HuSI-L23)CVCL_D0JB (iPS-14)CVCL_D0JC (iPS-27)
CVCL_D0JD (iPS-49)CVCL_D0JE (iPS-50)CVCL_D0JF (iPS-51)
CVCL_D0JG (iPS-59)CVCL_D0JH (iPS-60)CVCL_D0JI (iPS-62)
CVCL_D0JJ (iPS-71)CVCL_D0JK (iPS-72)CVCL_D0JL (iPS-76)
CVCL_RG58 (iPSC D1)CVCL_RG59 (iPSC D4)CVCL_RB14 (IRR-MRC-5)
CVCL_4W64 (L12.28-1/1)CVCL_U651 (L12.9-2(1))CVCL_U661 (L23immo)
CVCL_E260 (MRC-5 CV1)CVCL_2621 (MRC-5 pd13)CVCL_2622 (MRC-5 pd19)
CVCL_2623 (MRC-5 pd25)CVCL_2624 (MRC-5 pd30)CVCL_JF54 (MRC-5 PDL12)
CVCL_H749 (MRC-5 PDL27)CVCL_H748 (MRC-5 PDL40)CVCL_8B13 (MRC-5 PDL5.51)
CVCL_2625 (MRC-5 SV1 TG1)CVCL_2626 (MRC-5 SV1 TG2)CVCL_3027 (MRC-5-30)
CVCL_3028 (MRC-5-40)CVCL_3029 (MRC-5-50)CVCL_D690 (MRC-5V1)
CVCL_2627 (MRC-5V2)CVCL_D923 (MRC-iPS-1)CVCL_D924 (MRC-iPS-10)
CVCL_D925 (MRC-iPS-100)CVCL_D926 (MRC-iPS-101)CVCL_8343 (MRC-iPS-11)
CVCL_D927 (MRC-iPS-12)CVCL_D928 (MRC-iPS-13)CVCL_D929 (MRC-iPS-14)
CVCL_D930 (MRC-iPS-15)CVCL_D931 (MRC-iPS-16)CVCL_D932 (MRC-iPS-17)
CVCL_8367 (MRC-iPS-18)CVCL_D933 (MRC-iPS-19)CVCL_D934 (MRC-iPS-2)
CVCL_D935 (MRC-iPS-20)CVCL_D936 (MRC-iPS-21)CVCL_8426 (MRC-iPS-22)
CVCL_D937 (MRC-iPS-23)CVCL_D938 (MRC-iPS-24)CVCL_8433 (MRC-iPS-25)
CVCL_D939 (MRC-iPS-26)CVCL_8434 (MRC-iPS-27)CVCL_D940 (MRC-iPS-28)
CVCL_D941 (MRC-iPS-29)CVCL_D942 (MRC-iPS-3)CVCL_D943 (MRC-iPS-30)
CVCL_D944 (MRC-iPS-31)CVCL_D945 (MRC-iPS-32)CVCL_D946 (MRC-iPS-33)
CVCL_D947 (MRC-iPS-34)CVCL_D948 (MRC-iPS-35)CVCL_D949 (MRC-iPS-36)
CVCL_D950 (MRC-iPS-37)CVCL_D951 (MRC-iPS-38)CVCL_D952 (MRC-iPS-39)
CVCL_D953 (MRC-iPS-4)CVCL_D954 (MRC-iPS-40)CVCL_D955 (MRC-iPS-41)
CVCL_D956 (MRC-iPS-42)CVCL_D957 (MRC-iPS-43)CVCL_D958 (MRC-iPS-44)
CVCL_D959 (MRC-iPS-45)CVCL_D960 (MRC-iPS-46)CVCL_D961 (MRC-iPS-47)
CVCL_D962 (MRC-iPS-48)CVCL_D963 (MRC-iPS-49)CVCL_D964 (MRC-iPS-5)
CVCL_D965 (MRC-iPS-50)CVCL_D966 (MRC-iPS-51)CVCL_D967 (MRC-iPS-52)
CVCL_D968 (MRC-iPS-53)CVCL_D969 (MRC-iPS-54)CVCL_D970 (MRC-iPS-55)
CVCL_D971 (MRC-iPS-56)CVCL_D972 (MRC-iPS-57)CVCL_D973 (MRC-iPS-58)
CVCL_D974 (MRC-iPS-59)CVCL_D975 (MRC-iPS-6)CVCL_D976 (MRC-iPS-60)
CVCL_D977 (MRC-iPS-61)CVCL_D978 (MRC-iPS-62)CVCL_D979 (MRC-iPS-63)
CVCL_D980 (MRC-iPS-64)CVCL_D981 (MRC-iPS-65)CVCL_D982 (MRC-iPS-66)
CVCL_D983 (MRC-iPS-67)CVCL_D984 (MRC-iPS-68)CVCL_D985 (MRC-iPS-69)
CVCL_D986 (MRC-iPS-7)CVCL_D987 (MRC-iPS-70)CVCL_D988 (MRC-iPS-71)
CVCL_D989 (MRC-iPS-72)CVCL_D990 (MRC-iPS-73)CVCL_D991 (MRC-iPS-74)
CVCL_D992 (MRC-iPS-75)CVCL_D993 (MRC-iPS-76)CVCL_D994 (MRC-iPS-77)
CVCL_D995 (MRC-iPS-78)CVCL_D996 (MRC-iPS-79)CVCL_D997 (MRC-iPS-8)
CVCL_D998 (MRC-iPS-80)CVCL_D999 (MRC-iPS-81)CVCL_E000 (MRC-iPS-82)
CVCL_E001 (MRC-iPS-83)CVCL_E002 (MRC-iPS-84)CVCL_E003 (MRC-iPS-85)
CVCL_E004 (MRC-iPS-86)CVCL_E005 (MRC-iPS-87)CVCL_E006 (MRC-iPS-88)
CVCL_E007 (MRC-iPS-89)CVCL_E008 (MRC-iPS-9)CVCL_E009 (MRC-iPS-90)
CVCL_E010 (MRC-iPS-91)CVCL_E011 (MRC-iPS-92)CVCL_E012 (MRC-iPS-93)
CVCL_E013 (MRC-iPS-94)CVCL_E014 (MRC-iPS-95)CVCL_E015 (MRC-iPS-96)
CVCL_E016 (MRC-iPS-97)CVCL_E017 (MRC-iPS-98)CVCL_E018 (MRC-iPS-99)
CVCL_C975 (MRC5-iPS12)CVCL_C974 (MRC5-iPS17)CVCL_V234 (MRC5-iPS2)
CVCL_C1XY (MRC5-iPS7)CVCL_C976 (MRC5-RiPS-1.11)CVCL_C977 (MRC5-RiPS-1.2)
CVCL_C978 (MRC5-RiPS-1.3)CVCL_C979 (MRC5-RiPS-1.8)CVCL_C980 (MRC5-RiPS-1.9)
CVCL_B0XS (MRC5/ACE2)CVCL_Y625 (SR2)
Sex of cell Male
Age at sampling 14FW
Category Finite cell line
STR profile Source(s): AddexBio=T0016002/4922; ATCC=CCL-171; CCRID; CLS=300395; JCRB=IFO50073; JCRB=JCRB9008; KCLB=10171; PubMed=11416159; PubMed=21637780; PubMed=25877200; RCB=RCB0211

Markers:
AmelogeninX,Y
CSF1PO11,12
D2S133820
D3S135815,17
D5S81811,12
D6S104311,19
D7S82010,11
D8S117913
D12S39120,22
D13S31711,14
D16S5399,11
D18S5115,21
D19S43314,15
D21S1131.2
FGA21,23
Penta D12
Penta E12,16
TH018
TPOX8
vWA15

Run an STR similarity search on this cell line
Web pages Info; -; -; https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/mrc5/mrc5cells.html
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/number-of-population-doublings-pdl-for-atcc-ccl-171
Info; Evident; -; https://evidentscientific.com/en/microscope-resource/image-gallery/confocal/fibroblastindex
Info; StRAP; -; https://strap.nci.nih.gov/celline_detail.php?sample_id=68
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-380.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/MRC-5
Provider; Vircell; -; https://www.vircell.com/en/products/mrc-5-cell-line
Provider; WHO; -; https://www.who.int/teams/health-product-policy-and-standards/standards-and-specifications/norms-and-standards/who-reference-cell-banks
Publications

PubMed=4316953; DOI=10.1038/227168a0
J.P. Jacobs, C.M. Jones, J.P. Baille;
Characteristics of a human diploid cell designated MRC-5.
Nature 227:168-170(1970)

PubMed=1193162; DOI=10.1016/s0014-4827(75)80029-2
K.V.A. Thompson, Robin Holliday;
Chromosome changes during the in vitro ageing of MRC-5 human fibroblasts.
Exp. Cell Res. 96:1-6(1975)

PubMed=932048; DOI=10.1016/0092-1157(76)90018-4
J.P. Jacobs;
The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines.
J. Biol. Stand. 4:97-99(1976)

PubMed=1031681
J.P. Jacobs;
Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell strains.
Dev. Biol. Stand. 37:155-156(1976)

PubMed=211143; DOI=10.1128/jcm.7.4.368-371.1978; PMCID=PMC274969
Harvey M. Friedman, Celine Koropchak;
Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens.
J. Clin. Microbiol. 7:368-371(1978)

PubMed=113411; DOI=10.1016/S0092-1157(79)80043-8
J.P. Jacobs, A. John Garrett, Rosemary Merton;
Characteristics of a serially propagated human diploid cell designated MRC-9.
J. Biol. Stand. 7:113-122(1979)

PubMed=6971834; DOI=10.1080/09553008114550251
Alexander Cooke, William J. Harris;
Pyrimidine dimer excision in ageing human cells in tissue culture.
Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 39:223-226(1981)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222
Robert J. Hay, Carolyn D. Williams, Marvin L. Macy, Kantharajapura Subbegowda Lavappa;
Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.
Am. Rev. Respir. Dis. 125:222-232(1982)

PubMed=6538202; DOI=10.1083/jcb.98.3.1133; PMCID=PMC2113122
Bruno Azzarone, Horacio Guillermo Suarez, Maria Cristina Mingari, Lorenzo Moretta, Anthony Stephen Fauci;
4F2 monoclonal antibody recognizes a surface antigen on spread human fibroblasts of embryonic but not of adult origin.
J. Cell Biol. 98:1133-1137(1984)

PubMed=3335022
Michael C. Alley, Dominic A. Scudiero, Anne Monks, Miriam L. Hursey, Maciej J. Czerwinski, Donald L. Fine, Betty Jane Abbott, Joseph G. Mayo, Robert H. Shoemaker, Michael R. Boyd;
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=1965304; DOI=10.1002/elps.1150111203
Julio Enrique Celis, Kurt Dejgaard, Peder Madsen, Henrik Leffers, Borbala Gesser, Bent Honore, Hanne Holm Rasmussen, Eydfinnur Olsen, Jette B. Lauridsen, Gitte Ratz, Solveig Mouritzen ...Show all 17 authors... , Bodil Basse, Marianne Hellerup, Ariana Celis, Magda Puype, Jozef Van Damme, Joel Vandekerckhove; Show fewer authors
The MRC-5 human embryonal lung fibroblast two-dimensional gel cellular protein database: quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV40 transformed cells.
Electrophoresis 11:1072-1113(1990)

PubMed=1756776; DOI=10.1016/0531-5565(91)90033-i
Yoko Tasaki, Takane Ohara, Mari Yamamoto, Kiyotaka Yamamoto;
Isozyme polymorphisms in human diploid cell strains for research on cellular aging.
Exp. Gerontol. 26:441-451(1991)

PubMed=10752125
Galina Georgievna Poljanskaia, Tatiana N. Efremova, Galina Anatolyevna Sakuta;
The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability.
Tsitologiia 42:190-195(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

CLPUB00783
Glyn N. Stacey, J. Ross Hawkins, Penny Carter, Charles John Hunt, Lesley A. Young;
Replacement seed stock for MRC-5 cells.
(In technical document) World Health Organization publication WHO/BS/07.2077; pp.1-9; WHO; Geneva; Switzerland (2007)

PubMed=17395773; DOI=10.1634/stemcells.2006-0504
Kazuhiro Sudo, Megumi Kanno, Kenichi Miharada, Saeri Ogawa, Takashi Hiroyama, Kaoru Saijo, Yukio Nakamura;
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells 25:1610-1617(2007)

PubMed=19657000
Atchara Rubporn, Chantragan Srisomsap, Pantipa Subhasitanont, Daranee Chokchaichamnankit, Khajeelak Chiablaem, Jisnuson Svasti, Polkit Sangvanich;
Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.
Cancer Genomics Proteomics 6:229-237(2009)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085; PMCID=PMC3102737
Koichiro Nishino, Masashi Toyoda, Mayu Yamazaki-Inoue, Yoshihiro Fukawatase, Emi Chikazawa, Hironari Sakaguchi, Hidenori Akutsu, Akihiro Umezawa;
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:e1002085.1-e1002085.14(2011)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25903999; DOI=10.1002/biot.201400388
Yvonne Genzel;
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=26477663; DOI=10.1038/srep14988; PMCID=PMC4609952
Takuma Ojima, Eri Shibata, Shiho Saito, Masashi Toyoda, Hideki Nakajima, Mayu Yamazaki-Inoue, Yoshitaka Miyagawa, Nobutaka Kiyokawa, Jun-ichiro Fujimoto, Toshinori Sato, Akihiro Umezawa;
Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.
Sci. Rep. 5:14988.1-14988.13(2015)

CLPUB00391
Meredith Wadman;
The vaccine race: science; politics; and the human costs of defeating disease.
(In book) ISBN 9780525427537; pp.1-436; Viking; New York; USA (2016)

PubMed=31869419; DOI=10.1084/jem.20191164; PMCID=PMC7062530
Maren Lubke, Stefanie Spalt, Daniel Johannes Kowalewski, Cosima Zimmermann, Liane Bauersfeld, Annika Nelde, Leon Bichmann, Ana Marcu, Janet Kerstin Peper, Oliver Kohlbacher, Juliane Sarah Walz ...Show all 16 authors... , Vu Thuy Khanh Le-Trilling, Hartmut Hengel, Hans-Georg Rammensee, Stefan Stevanovic, Anne Halenius; Show fewer authors
Identification of HCMV-derived T cell epitopes in seropositive individuals through viral deletion models.
J. Exp. Med. 217:e20191164.1-e20191164.14(2020)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) AddexBio; T0016002/4922
ATCC; CCL-171
BCRC; 60023
BCRJ; 0180
CCTCC; GDC0032
CLS; 300395
ECACC; 97112601 - Discontinued
IBRC; C10313
ICLC; HL95001
IZSLER; BS CL 68
JCRB; IFO50073
JCRB; JCRB9008
KCB; KCB 200412YJ
KCB; KCB 89023YJ
KCLB; 10171
NCBI_Iran; C125
RCB; RCB0211
RCB; RCB0218
Cell line databases/resources CLO; CLO_0007865
CLO; CLO_0050055
MCCL; MCC:0000336
CLDB; cl3563
CLDB; cl3564
CLDB; cl3566
CLDB; cl3567
CLDB; cl3568
CLDB; cl5168
CCRID; 1101HUM-PUMC000044
CCRID; 1102HUM-NIFDC00002
CCRID; 3101HUMGNHu41
CCRID; 3101HUMSCSP5040
CCRID; 4201HUM-CCTCC00032
CCRID; 5301HUM-KCB89023YJ
FCS-free; 179-2-338-1-16-3
Lonza; 52
Anatomy/cell type resources BTO; BTO_0001590
Biological sample resources BioSample; SAMN01821580
BioSample; SAMN01821702
BioSample; SAMN01821735
BioSample; SAMN03473183
Chemistry resources ChEMBL-Cells; CHEMBL3308499
ChEMBL-Targets; CHEMBL614181
PubChem_Cell_line; CVCL_0440
Encyclopedic resources Wikidata; Q3273166
Experimental variables resources EFO; EFO_0002835
Gene expression databases GEO; GSM248209
GEO; GSM248210
GEO; GSM520665
GEO; GSM520666
GEO; GSM520667
GEO; GSM579888
GEO; GSM579889
GEO; GSM607969
GEO; GSM3738752
GEO; GSM3738753
Proteomic databases PRIDE; PXD001909
PRIDE; PXD013120
PRIDE; PXD023286
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number44