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Cellosaurus MRC-5 (CVCL_0440)

[Text version]
Cell line name MRC-5
Synonyms MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5
Accession CVCL_0440
Resource Identification Initiative To cite this cell line use: MRC-5 (RRID:CVCL_0440)
Comments Group: Vaccine production cell line.
Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies).
Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta.
Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax).
Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax).
Senescence: Capable of attaining 42-46 population doublings before the onset of senescence (ATCC=CCL-171). Senesces at 26 PDL (PubMed=17395773).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 45 hours (Note=Lot 08072009), ~40 hours (Note=Lot 02272015) (JCRB=IFO50073); 36 hours (Note=Lot 041392), ~5 days (Note=Lot 031098), ~2 days (Note=Lot 05192003), ~1.5 days (Note=Lot 05202005), ~34 hours (Note=Lot 06022011), ~38 hours (Note=Lot 04212016) (JCRB=JCRB9008).
Omics: DNA methylation analysis.
Omics: Glycosphingolipids analysis.
Omics: Proteome analysis by 2D-DE.
Omics: Proteome analysis by 2D-DE/MS.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: MCR-5; Note=Occasionally.
Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
Cell type: Fibroblast of lung; CL=CL_0002553.
HLA typing Source: PubMed=31869419
Class I
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_2264 (1221)CVCL_2837 (A9(Neo1))CVCL_2838 (A9(Neo11))
CVCL_2844 (A9(Neo19))CVCL_2846 (A9(Neo20))CVCL_2848 (A9(Neo4))
CVCL_WT86 (AG27415)CVCL_F664 (Flow13000)CVCL_JG29 (HuS-L12/10)
CVCL_4W54 (HuSI-L23)CVCL_D0JB (iPS-14)CVCL_D0JC (iPS-27)
CVCL_D0JD (iPS-49)CVCL_D0JE (iPS-50)CVCL_D0JF (iPS-51)
CVCL_D0JG (iPS-59)CVCL_D0JH (iPS-60)CVCL_D0JI (iPS-62)
CVCL_D0JJ (iPS-71)CVCL_D0JK (iPS-72)CVCL_D0JL (iPS-76)
CVCL_4W64 (L12.28-1/1)CVCL_U651 (L12.9-2(1))CVCL_U661 (L23immo)
CVCL_E260 (MRC-5 CV1)CVCL_2621 (MRC-5 pd13)CVCL_2622 (MRC-5 pd19)
CVCL_2623 (MRC-5 pd25)CVCL_2624 (MRC-5 pd30)CVCL_JF54 (MRC-5 PDL12)
CVCL_H749 (MRC-5 PDL27)CVCL_H748 (MRC-5 PDL40)CVCL_8B13 (MRC-5 PDL5.51)
CVCL_2625 (MRC-5 SV1 TG1)CVCL_2626 (MRC-5 SV1 TG2)CVCL_3027 (MRC-5-30)
CVCL_3028 (MRC-5-40)CVCL_3029 (MRC-5-50)CVCL_D690 (MRC-5V1)
CVCL_2627 (MRC-5V2)CVCL_D923 (MRC-iPS-1)CVCL_D924 (MRC-iPS-10)
CVCL_D925 (MRC-iPS-100)CVCL_D926 (MRC-iPS-101)CVCL_8343 (MRC-iPS-11)
CVCL_D927 (MRC-iPS-12)CVCL_D928 (MRC-iPS-13)CVCL_D929 (MRC-iPS-14)
CVCL_D930 (MRC-iPS-15)CVCL_D931 (MRC-iPS-16)CVCL_D932 (MRC-iPS-17)
CVCL_8367 (MRC-iPS-18)CVCL_D933 (MRC-iPS-19)CVCL_D934 (MRC-iPS-2)
CVCL_D935 (MRC-iPS-20)CVCL_D936 (MRC-iPS-21)CVCL_8426 (MRC-iPS-22)
CVCL_D937 (MRC-iPS-23)CVCL_D938 (MRC-iPS-24)CVCL_8433 (MRC-iPS-25)
CVCL_D939 (MRC-iPS-26)CVCL_8434 (MRC-iPS-27)CVCL_D940 (MRC-iPS-28)
CVCL_D941 (MRC-iPS-29)CVCL_D942 (MRC-iPS-3)CVCL_D943 (MRC-iPS-30)
CVCL_D944 (MRC-iPS-31)CVCL_D945 (MRC-iPS-32)CVCL_D946 (MRC-iPS-33)
CVCL_D947 (MRC-iPS-34)CVCL_D948 (MRC-iPS-35)CVCL_D949 (MRC-iPS-36)
CVCL_D950 (MRC-iPS-37)CVCL_D951 (MRC-iPS-38)CVCL_D952 (MRC-iPS-39)
CVCL_D953 (MRC-iPS-4)CVCL_D954 (MRC-iPS-40)CVCL_D955 (MRC-iPS-41)
CVCL_D956 (MRC-iPS-42)CVCL_D957 (MRC-iPS-43)CVCL_D958 (MRC-iPS-44)
CVCL_D959 (MRC-iPS-45)CVCL_D960 (MRC-iPS-46)CVCL_D961 (MRC-iPS-47)
CVCL_D962 (MRC-iPS-48)CVCL_D963 (MRC-iPS-49)CVCL_D964 (MRC-iPS-5)
CVCL_D965 (MRC-iPS-50)CVCL_D966 (MRC-iPS-51)CVCL_D967 (MRC-iPS-52)
CVCL_D968 (MRC-iPS-53)CVCL_D969 (MRC-iPS-54)CVCL_D970 (MRC-iPS-55)
CVCL_D971 (MRC-iPS-56)CVCL_D972 (MRC-iPS-57)CVCL_D973 (MRC-iPS-58)
CVCL_D974 (MRC-iPS-59)CVCL_D975 (MRC-iPS-6)CVCL_D976 (MRC-iPS-60)
CVCL_D977 (MRC-iPS-61)CVCL_D978 (MRC-iPS-62)CVCL_D979 (MRC-iPS-63)
CVCL_D980 (MRC-iPS-64)CVCL_D981 (MRC-iPS-65)CVCL_D982 (MRC-iPS-66)
CVCL_D983 (MRC-iPS-67)CVCL_D984 (MRC-iPS-68)CVCL_D985 (MRC-iPS-69)
CVCL_D986 (MRC-iPS-7)CVCL_D987 (MRC-iPS-70)CVCL_D988 (MRC-iPS-71)
CVCL_D989 (MRC-iPS-72)CVCL_D990 (MRC-iPS-73)CVCL_D991 (MRC-iPS-74)
CVCL_D992 (MRC-iPS-75)CVCL_D993 (MRC-iPS-76)CVCL_D994 (MRC-iPS-77)
CVCL_D995 (MRC-iPS-78)CVCL_D996 (MRC-iPS-79)CVCL_D997 (MRC-iPS-8)
CVCL_D998 (MRC-iPS-80)CVCL_D999 (MRC-iPS-81)CVCL_E000 (MRC-iPS-82)
CVCL_E001 (MRC-iPS-83)CVCL_E002 (MRC-iPS-84)CVCL_E003 (MRC-iPS-85)
CVCL_E004 (MRC-iPS-86)CVCL_E005 (MRC-iPS-87)CVCL_E006 (MRC-iPS-88)
CVCL_E007 (MRC-iPS-89)CVCL_E008 (MRC-iPS-9)CVCL_E009 (MRC-iPS-90)
CVCL_E010 (MRC-iPS-91)CVCL_E011 (MRC-iPS-92)CVCL_E012 (MRC-iPS-93)
CVCL_E013 (MRC-iPS-94)CVCL_E014 (MRC-iPS-95)CVCL_E015 (MRC-iPS-96)
CVCL_E016 (MRC-iPS-97)CVCL_E017 (MRC-iPS-98)CVCL_E018 (MRC-iPS-99)
CVCL_C975 (MRC5-iPS12)CVCL_C974 (MRC5-iPS17)CVCL_V234 (MRC5-iPS2)
CVCL_C1XY (MRC5-iPS7)CVCL_C976 (MRC5-RiPS-1.11)CVCL_C977 (MRC5-RiPS-1.2)
CVCL_C978 (MRC5-RiPS-1.3)CVCL_C979 (MRC5-RiPS-1.8)CVCL_C980 (MRC5-RiPS-1.9)
Sex of cell Male
Age at sampling 14FW
Category Finite cell line
STR profile Source(s): AddexBio=T0016002/4922; ATCC=CCL-171; CCRID; JCRB=IFO50073; JCRB=JCRB9008; KCLB=10171; PubMed=11416159; PubMed=21637780; PubMed=25877200; RCB=RCB0211

Penta D12
Penta E12,16

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/MRC-5

PubMed=4316953; DOI=10.1038/227168a0
Jacobs J.P., Jones C.M., Baille J.P.
Characteristics of a human diploid cell designated MRC-5.
Nature 227:168-170(1970)

PubMed=932048; DOI=10.1016/0092-1157(76)90018-4
Jacobs J.P.
The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines.
J. Biol. Stand. 4:97-99(1976)

Jacobs J.P.
Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell strains.
Dev. Biol. Stand. 37:155-156(1976)

PubMed=211143; DOI=10.1128/jcm.7.4.368-371.1978
Friedman H.M., Koropchak C.
Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens.
J. Clin. Microbiol. 7:368-371(1978)

PubMed=113411; DOI=10.1016/S0092-1157(79)80043-8
Jacobs J.P., Garrett A.J., Merton R.
Characteristics of a serially propagated human diploid cell designated MRC-9.
J. Biol. Stand. 7:113-122(1979)

PubMed=6971834; DOI=10.1080/09553008114550251
Cooke A., Harris W.J.
Pyrimidine dimer excision in ageing human cells in tissue culture.
Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 39:223-226(1981)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222
Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.
Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.
Am. Rev. Respir. Dis. 125:222-232(1982)

PubMed=6538202; DOI=10.1083/jcb.98.3.1133
Azzarone B., Suarez H.G., Mingari M.C., Moretta L., Fauci A.S.
4F2 monoclonal antibody recognizes a surface antigen on spread human fibroblasts of embryonic but not of adult origin.
J. Cell Biol. 98:1133-1137(1984)

Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=1965304; DOI=10.1002/elps.1150111203
Celis J.E., Dejgaard K., Madsen P., Leffers H., Gesser B., Honore B., Rasmussen H.H., Olsen E., Lauridsen J.B., Ratz G., Mouritzen S., Basse B., Hellerup M., Celis A., Puype M., Van Damme J., Vandekerckhove J.
The MRC-5 human embryonal lung fibroblast two-dimensional gel cellular protein database: quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV40 transformed cells.
Electrophoresis 11:1072-1113(1990)

Poljanskaia G.G., Efremova T.N., Sakuta G.A.
The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability.
Tsitologiia 42:190-195(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=17395773; DOI=10.1634/stemcells.2006-0504
Sudo K., Kanno M., Miharada K., Ogawa S., Hiroyama T., Saijo K., Nakamura Y.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells 25:1610-1617(2007)

Rubporn A., Srisomsap C., Subhasitanont P., Chokchaichamnankit D., Chiablaem K., Svasti J., Sangvanich P.
Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.
Cancer Genomics Proteomics 6:229-237(2009)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085
Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E., Sakaguchi H., Akutsu H., Umezawa A.
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:E1002085-E1002085(2011)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=26477663; DOI=10.1038/srep14988
Ojima T., Shibata E., Saito S., Toyoda M., Nakajima H., Yamazaki-Inoue M., Miyagawa Y., Kiyokawa N., Fujimoto J.-i., Sato T., Umezawa A.
Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.
Sci. Rep. 5:14988-14988(2015)

Wadman M.
The vaccine race: science, politics, and the human costs of defeating disease.
(In) ISBN 9780525427537; pp.1-436; Viking; New York (2016)

PubMed=31869419; DOI=10.1084/jem.20191164
Lubke M., Spalt S., Kowalewski D.J., Zimmermann C., Bauersfeld L., Nelde A., Bichmann L., Marcu A., Peper J.K., Kohlbacher O., Walz J.S., Le-Trilling V.T.K., Hengel H., Rammensee H.-G., Stevanovic S., Halenius A.
Identification of HCMV-derived T cell epitopes in seropositive individuals through viral deletion models.
J. Exp. Med. 217:20191164.1-20191164.14(2020)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cell line collections (Providers) AddexBio; T0016002/4922
BCRC; 60023
BCRJ; 0180
CLS; 300395 - Discontinued
ECACC; 97112601 - Discontinued
IBRC; C10313
ICLC; HL95001
JCRB; IFO50073
KCB; KCB 89023YJ
KCB; KCB 200412YJ
KCLB; 10171
NCBI_Iran; C125
RCB; RCB0211
RCB; RCB0218
Cell line databases/resources CLO; CLO_0007865
CLO; CLO_0050055
MCCL; MCC:0000336
CLDB; cl3563
CLDB; cl3564
CLDB; cl3566
CLDB; cl3567
CLDB; cl3568
CLDB; cl5168
CCRID; 1101HUM-PUMC000044
FCS-free; 179-2-338-1-16-3
Lonza; 52
Anatomy/cell type resources BTO; BTO:0001590
Biological sample resources BioSample; SAMN01821580
BioSample; SAMN01821702
BioSample; SAMN01821735
BioSample; SAMN03473183
Chemistry resources ChEMBL-Cells; CHEMBL3308499
ChEMBL-Targets; CHEMBL614181
PubChem_Cell_line; CVCL_0440
Encyclopedic resources Wikidata; Q3273166
Experimental variables resources EFO; EFO_0002835
Gene expression databases GEO; GSM248209
GEO; GSM248210
GEO; GSM520665
GEO; GSM520666
GEO; GSM520667
GEO; GSM579888
GEO; GSM579889
GEO; GSM607969
GEO; GSM3738752
GEO; GSM3738753
Proteomic databases PRIDE; PXD001909
PRIDE; PXD013120
PRIDE; PXD023286
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number40