ID   MRC-5
AC   CVCL_0440
SY   MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5
DR   BTO; BTO_0001590
DR   CLO; CLO_0007865
DR   CLO; CLO_0050055
DR   EFO; EFO_0002835
DR   MCCL; MCC:0000336
DR   CLDB; cl3563
DR   CLDB; cl3564
DR   CLDB; cl3566
DR   CLDB; cl3567
DR   CLDB; cl3568
DR   CLDB; cl5168
DR   AddexBio; T0016002/4922
DR   ATCC; CCL-171
DR   BCRC; 60023
DR   BCRJ; 0180
DR   BioSample; SAMN01821580
DR   BioSample; SAMN01821702
DR   BioSample; SAMN01821735
DR   BioSample; SAMN03473183
DR   CCRID; 1101HUM-PUMC000044
DR   CCRID; 1102HUM-NIFDC00002
DR   CCRID; 3101HUMGNHu41
DR   CCRID; 3101HUMSCSP5040
DR   CCRID; 4201HUM-CCTCC00032
DR   CCRID; 5301HUM-KCB89023YJ
DR   CCTCC; GDC0032
DR   ChEMBL-Cells; CHEMBL3308499
DR   ChEMBL-Targets; CHEMBL614181
DR   CLS; 300395
DR   ECACC; 97112601
DR   FCS-free; 179-2-338-1-16-3
DR   GEO; GSM248209
DR   GEO; GSM248210
DR   GEO; GSM520665
DR   GEO; GSM520666
DR   GEO; GSM520667
DR   GEO; GSM579888
DR   GEO; GSM579889
DR   GEO; GSM607969
DR   GEO; GSM3738752
DR   GEO; GSM3738753
DR   IBRC; C10313
DR   ICLC; HL95001
DR   IZSLER; BS CL 68
DR   JCRB; IFO50073
DR   JCRB; JCRB9008
DR   KCB; KCB 200412YJ
DR   KCB; KCB 89023YJ
DR   KCLB; 10171
DR   Lonza; 52
DR   NCBI_Iran; C125
DR   PRIDE; PXD001909
DR   PRIDE; PXD013120
DR   PRIDE; PXD023286
DR   PubChem_Cell_line; CVCL_0440
DR   RCB; RCB0211
DR   RCB; RCB0218
DR   Wikidata; Q3273166
RX   CelloPub=CLPUB00391;
RX   CelloPub=CLPUB00783;
RX   PubMed=113411;
RX   PubMed=211143;
RX   PubMed=932048;
RX   PubMed=1031681;
RX   PubMed=1193162;
RX   PubMed=1756776;
RX   PubMed=1965304;
RX   PubMed=3335022;
RX   PubMed=4316953;
RX   PubMed=6538202;
RX   PubMed=6971834;
RX   PubMed=7065527;
RX   PubMed=10752125;
RX   PubMed=11416159;
RX   PubMed=17395773;
RX   PubMed=19657000;
RX   PubMed=21637780;
RX   PubMed=25877200;
RX   PubMed=25903999;
RX   PubMed=26477663;
RX   PubMed=31869419;
RX   PubMed=33389257;
WW   Info; -; -; https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/mrc5/mrc5cells.html
WW   Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/number-of-population-doublings-pdl-for-atcc-ccl-171
WW   Info; Evident; -; https://evidentscientific.com/en/microscope-resource/image-gallery/confocal/fibroblastindex
WW   Info; StRAP; -; https://strap.nci.nih.gov/celline_detail.php?sample_id=68
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-380.html
WW   Info; Wikipedia; -; https://en.wikipedia.org/wiki/MRC-5
WW   Provider; Vircell; -; https://www.vircell.com/en/products/mrc-5-cell-line
WW   Provider; WHO; -; https://www.who.int/teams/health-product-policy-and-standards/standards-and-specifications/norms-and-standards/who-reference-cell-banks
CC   Group: Vaccine production cell line.
CC   Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies).
CC   Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta.
CC   Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax).
CC   Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax).
CC   Senescence: Capable of attaining 42-46 population doublings before the onset of senescence (ATCC=CCL-171). Senesces at 26 PDL (PubMed=17395773).
CC   Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
CC   Doubling time: 45 hours (Note=Lot 08072009), ~40 hours (Note=Lot 02272015) (JCRB=IFO50073); 36 hours (Note=Lot 041392), ~5 days (Note=Lot 031098), ~2 days (Note=Lot 05192003), ~1.5 days (Note=Lot 05202005), ~34 hours (Note=Lot 06022011), ~38 hours (Note=Lot 04212016) (JCRB=JCRB9008).
CC   HLA typing: A*02:01,29:02; B*07:02,44:02; C*05:01,07:02 (PubMed=31869419).
CC   HLA typing: A*02:01:01,29:02:01; B*07:02:01,44:02:01; C*05:01:01,07:02:01; DPB1*04:01:01; DQA1*01:02:01,03:03:01; DQB1*03:01:01,06:02:01; DRB1*04:08:01,15:01:01G (CLS=300395).
CC   Omics: Genomics; DNA methylation analysis.
CC   Omics: Glycomics; Glycosphingolipids.
CC   Omics: Proteomics; 2DE-MS.
CC   Omics: Proteomics; 2DE.
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Transcriptomics; RNAseq.
CC   Misspelling: MCR-5; Note=Occasionally.
CC   Discontinued: ECACC; 97112601; true.
CC   Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
CC   Cell type: Fibroblast of lung; CL=CL_0002553.
ST   Source(s): AddexBio=T0016002/4922; ATCC=CCL-171; CCRID; CLS=300395; JCRB=IFO50073; JCRB=JCRB9008; KCLB=10171; PubMed=11416159; PubMed=21637780; PubMed=25877200; RCB=RCB0211
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D12S391: 20,22
ST   D13S317: 11,14
ST   D16S539: 9,11
ST   D18S51: 15,21
ST   D19S433: 14,15
ST   D21S11: 31.2
ST   D2S1338: 20
ST   D3S1358: 15,17
ST   D5S818: 11,12
ST   D6S1043: 11,19
ST   D7S820: 10,11
ST   D8S1179: 13
ST   FGA: 21,23
ST   Penta D: 12
ST   Penta E: 12,16
ST   TH01: 8
ST   TPOX: 8
ST   vWA: 15
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   14FW
CA   Finite cell line
DT   Created: 04-04-12; Last updated: 14-08-25; Version: 44
//
RX   CelloPub=CLPUB00391;
RA   Wadman, Meredith
RT   "The vaccine race: science; politics; and the human costs of defeating
RT   disease.";
RL   (In book) ISBN 9780525427537; pp.1-436; Viking; New York; USA (2016).
//
RX   CelloPub=CLPUB00783;
RA   Stacey, Glyn N.
RA   Hawkins, J. Ross
RA   Carter, Penny
RA   Hunt, Charles John
RA   Young, Lesley A.
RT   "Replacement seed stock for MRC-5 cells.";
RL   (In technical document) World Health Organization publication WHO/BS/07.2077; pp.1-9; WHO; Geneva; Switzerland (2007).
//
RX   PubMed=113411; DOI=10.1016/S0092-1157(79)80043-8;
RA   Jacobs, J.P.
RA   Garrett, A. John
RA   Merton, Rosemary
RT   "Characteristics of a serially propagated human diploid cell
RT   designated MRC-9.";
RL   J. Biol. Stand. 7:113-122(1979).
//
RX   PubMed=211143; DOI=10.1128/jcm.7.4.368-371.1978; PMCID=PMC274969;
RA   Friedman, Harvey M.
RA   Koropchak, Celine
RT   "Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of
RT   viruses from clinical specimens.";
RL   J. Clin. Microbiol. 7:368-371(1978).
//
RX   PubMed=932048; DOI=10.1016/0092-1157(76)90018-4;
RA   Jacobs, J.P.
RT   "The status of human diploid cell strain MRC-5 as an approved
RT   substrate for the production of viral vaccines.";
RL   J. Biol. Stand. 4:97-99(1976).
//
RX   PubMed=1031681;
RA   Jacobs, J.P.
RT   "Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell
RT   strains.";
RL   Dev. Biol. Stand. 37:155-156(1976).
//
RX   PubMed=1193162; DOI=10.1016/s0014-4827(75)80029-2;
RA   Thompson, K.V.A.
RA   Holliday, Robin
RT   "Chromosome changes during the in vitro ageing of MRC-5 human
RT   fibroblasts.";
RL   Exp. Cell Res. 96:1-6(1975).
//
RX   PubMed=1756776; DOI=10.1016/0531-5565(91)90033-i;
RA   Tasaki, Yoko
RA   Ohara, Takane
RA   Yamamoto, Mari
RA   Yamamoto, Kiyotaka
RT   "Isozyme polymorphisms in human diploid cell strains for research on
RT   cellular aging.";
RL   Exp. Gerontol. 26:441-451(1991).
//
RX   PubMed=1965304; DOI=10.1002/elps.1150111203;
RA   Celis, Julio Enrique
RA   Dejgaard, Kurt
RA   Madsen, Peder
RA   Leffers, Henrik
RA   Gesser, Borbala
RA   Honore, Bent
RA   Rasmussen, Hanne Holm
RA   Olsen, Eydfinnur
RA   Lauridsen, Jette B.
RA   Ratz, Gitte
RA   Mouritzen, Solveig
RA   Basse, Bodil
RA   Hellerup, Marianne
RA   Celis, Ariana
RA   Puype, Magda
RA   Van Damme, Jozef
RA   Vandekerckhove, Joel
RT   "The MRC-5 human embryonal lung fibroblast two-dimensional gel
RT   cellular protein database: quantitative identification of polypeptides
RT   whose relative abundance differs between quiescent, proliferating and
RT   SV40 transformed cells.";
RL   Electrophoresis 11:1072-1113(1990).
//
RX   PubMed=3335022;
RA   Alley, Michael C.
RA   Scudiero, Dominic A.
RA   Monks, Anne
RA   Hursey, Miriam L.
RA   Czerwinski, Maciej J.
RA   Fine, Donald L.
RA   Abbott, Betty Jane
RA   Mayo, Joseph G.
RA   Shoemaker, Robert H.
RA   Boyd, Michael R.
RT   "Feasibility of drug screening with panels of human tumor cell lines
RT   using a microculture tetrazolium assay.";
RL   Cancer Res. 48:589-601(1988).
//
RX   PubMed=4316953; DOI=10.1038/227168a0;
RA   Jacobs, J.P.
RA   Jones, C.M.
RA   Baille, J.P.
RT   "Characteristics of a human diploid cell designated MRC-5.";
RL   Nature 227:168-170(1970).
//
RX   PubMed=6538202; DOI=10.1083/jcb.98.3.1133; PMCID=PMC2113122;
RA   Azzarone, Bruno
RA   Suarez, Horacio Guillermo
RA   Mingari, Maria Cristina
RA   Moretta, Lorenzo
RA   Fauci, Anthony Stephen
RT   "4F2 monoclonal antibody recognizes a surface antigen on spread human
RT   fibroblasts of embryonic but not of adult origin.";
RL   J. Cell Biol. 98:1133-1137(1984).
//
RX   PubMed=6971834; DOI=10.1080/09553008114550251;
RA   Cooke, Alexander
RA   Harris, William J.
RT   "Pyrimidine dimer excision in ageing human cells in tissue culture.";
RL   Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 39:223-226(1981).
//
RX   PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222;
RA   Hay, Robert J.
RA   Williams, Carolyn D.
RA   Macy, Marvin L.
RA   Lavappa, Kantharajapura Subbegowda
RT   "Cultured cell lines for research on pulmonary physiology available
RT   through the American Type Culture Collection.";
RL   Am. Rev. Respir. Dis. 125:222-232(1982).
//
RX   PubMed=10752125;
RA   Poljanskaia, Galina Georgievna
RA   Efremova, Tatiana N.
RA   Sakuta, Galina Anatolyevna
RT   "The effect of mycoplasmal contamination of human embryonic lung cell
RT   line MRC-5 on the karyotypic variability.";
RL   Tsitologiia 42:190-195(2000).
//
RX   PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459;
RA   Masters, John R.W.
RA   Thomson, Jim A.
RA   Daly-Burns, Bernadette
RA   Reid, Yvonne A.
RA   Dirks, Wilhelm Gerhard
RA   Packer, Phil
RA   Toji, Lorraine H.
RA   Ohno, Tadao
RA   Tanabe, Hideyuki
RA   Arlett, Colin Francis
RA   Kelland, Lloyd R.
RA   Harrison, Maureen
RA   Virmani, Arvind K.
RA   Ward, Timothy H.
RA   Ayres, Karen L.
RA   Debenham, Paul G.
RT   "Short tandem repeat profiling provides an international reference
RT   standard for human cell lines.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
//
RX   PubMed=17395773; DOI=10.1634/stemcells.2006-0504;
RA   Sudo, Kazuhiro
RA   Kanno, Megumi
RA   Miharada, Kenichi
RA   Ogawa, Saeri
RA   Hiroyama, Takashi
RA   Saijo, Kaoru
RA   Nakamura, Yukio
RT   "Mesenchymal progenitors able to differentiate into osteogenic,
RT   chondrogenic, and/or adipogenic cells in vitro are present in most
RT   primary fibroblast-like cell populations.";
RL   Stem Cells 25:1610-1617(2007).
//
RX   PubMed=19657000;
RA   Rubporn, Atchara
RA   Srisomsap, Chantragan
RA   Subhasitanont, Pantipa
RA   Chokchaichamnankit, Daranee
RA   Chiablaem, Khajeelak
RA   Svasti, Jisnuson
RA   Sangvanich, Polkit
RT   "Comparative proteomic analysis of lung cancer cell line and lung
RT   fibroblast cell line.";
RL   Cancer Genomics Proteomics 6:229-237(2009).
//
RX   PubMed=21637780; DOI=10.1371/journal.pgen.1002085; PMCID=PMC3102737;
RA   Nishino, Koichiro
RA   Toyoda, Masashi
RA   Yamazaki-Inoue, Mayu
RA   Fukawatase, Yoshihiro
RA   Chikazawa, Emi
RA   Sakaguchi, Hironari
RA   Akutsu, Hidenori
RA   Umezawa, Akihiro
RT   "DNA methylation dynamics in human induced pluripotent stem cells over
RT   time.";
RL   PLoS Genet. 7:e1002085.1-e1002085.14(2011).
//
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu, Ma-Mie
RA   Selvaraj, Suresh K.
RA   Liang-Chu, May M.Y.
RA   Aghajani, Sahar
RA   Busse, Matthew
RA   Yuan, Jean
RA   Lee, Genee
RA   Peale, Franklin V.
RA   Klijn, Christiaan
RA   Bourgon, Richard
RA   Kaminker, Joshua S.
RA   Neve, Richard M.
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
//
RX   PubMed=25903999; DOI=10.1002/biot.201400388;
RA   Genzel, Yvonne
RT   "Designing cell lines for viral vaccine production: where do we
RT   stand?";
RL   Biotechnol. J. 10:728-740(2015).
//
RX   PubMed=26477663; DOI=10.1038/srep14988; PMCID=PMC4609952;
RA   Ojima, Takuma
RA   Shibata, Eri
RA   Saito, Shiho
RA   Toyoda, Masashi
RA   Nakajima, Hideki
RA   Yamazaki-Inoue, Mayu
RA   Miyagawa, Yoshitaka
RA   Kiyokawa, Nobutaka
RA   Fujimoto, Jun-ichiro
RA   Sato, Toshinori
RA   Umezawa, Akihiro
RT   "Glycolipid dynamics in generation and differentiation of induced
RT   pluripotent stem cells.";
RL   Sci. Rep. 5:14988.1-14988.13(2015).
//
RX   PubMed=31869419; DOI=10.1084/jem.20191164; PMCID=PMC7062530;
RA   Lubke, Maren
RA   Spalt, Stefanie
RA   Kowalewski, Daniel Johannes
RA   Zimmermann, Cosima
RA   Bauersfeld, Liane
RA   Nelde, Annika
RA   Bichmann, Leon
RA   Marcu, Ana
RA   Peper, Janet Kerstin
RA   Kohlbacher, Oliver
RA   Walz, Juliane Sarah
RA   Le-Trilling, Vu Thuy Khanh
RA   Hengel, Hartmut
RA   Rammensee, Hans-Georg
RA   Stevanovic, Stefan
RA   Halenius, Anne
RT   "Identification of HCMV-derived T cell epitopes in seropositive
RT   individuals through viral deletion models.";
RL   J. Exp. Med. 217:e20191164.1-e20191164.14(2020).
//
RX   PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494;
RA   Wurtz, Nathalie
RA   Penant, Gwilherm
RA   Jardot, Priscilla
RA   Duclos, Nathalie
RA   La Scola, Bernard
RT   "Culture of SARS-CoV-2 in a panel of laboratory cell lines,
RT   permissivity, and differences in growth profile.";
RL   Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021).
//