ID   MRC-5
AC   CVCL_0440
SY   MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5
DR   BTO; BTO:0001590
DR   CLO; CLO_0007865
DR   CLO; CLO_0050055
DR   EFO; EFO_0002835
DR   MCCL; MCC:0000336
DR   CLDB; cl3563
DR   CLDB; cl3564
DR   CLDB; cl3566
DR   CLDB; cl3567
DR   CLDB; cl3568
DR   CLDB; cl5168
DR   AddexBio; T0016002/4922
DR   ATCC; CCL-171
DR   BCRC; 60023
DR   BCRJ; 0180
DR   BioSample; SAMN01821580
DR   BioSample; SAMN01821702
DR   BioSample; SAMN01821735
DR   BioSample; SAMN03473183
DR   CCRID; 1101HUM-PUMC000044
DR   CCRID; 1102HUM-NIFDC00002
DR   CCRID; 4201HUM-CCTCC00032
DR   CCRID; 5301HUM-KCB89023YJ
DR   ChEMBL-Cells; CHEMBL3308499
DR   ChEMBL-Targets; CHEMBL614181
DR   CLS; 300395
DR   ECACC; 97112601
DR   FCS-free; 179-2-338-1-16-3
DR   GEO; GSM248209
DR   GEO; GSM248210
DR   GEO; GSM520665
DR   GEO; GSM520666
DR   GEO; GSM520667
DR   GEO; GSM579888
DR   GEO; GSM579889
DR   GEO; GSM607969
DR   GEO; GSM3738752
DR   GEO; GSM3738753
DR   IBRC; C10313
DR   ICLC; HL95001
DR   JCRB; IFO50073
DR   KCB; KCB 89023YJ
DR   KCB; KCB 200412YJ
DR   KCLB; 10171
DR   Lonza; 52
DR   NCBI_Iran; C125
DR   PRIDE; PXD001909
DR   PRIDE; PXD013120
DR   PRIDE; PXD023286
DR   PubChem_Cell_line; CVCL_0440
DR   RCB; RCB0211
DR   RCB; RCB0218
DR   Wikidata; Q3273166
RX   CelloPub=CLPUB00391;
RX   PubMed=113411;
RX   PubMed=211143;
RX   PubMed=932048;
RX   PubMed=1031681;
RX   PubMed=1965304;
RX   PubMed=3335022;
RX   PubMed=4316953;
RX   PubMed=6538202;
RX   PubMed=6971834;
RX   PubMed=7065527;
RX   PubMed=10752125;
RX   PubMed=11416159;
RX   PubMed=17395773;
RX   PubMed=19657000;
RX   PubMed=21637780;
RX   PubMed=25877200;
RX   PubMed=25903999;
RX   PubMed=26477663;
RX   PubMed=31869419;
RX   PubMed=33389257;
CC   Group: Vaccine production cell line.
CC   Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies).
CC   Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta.
CC   Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax).
CC   Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax).
CC   Senescence: Capable of attaining 42-46 population doublings before the onset of senescence (ATCC=CCL-171). Senesces at 26 PDL (PubMed=17395773).
CC   Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
CC   Doubling time: 45 hours (Note=Lot 08072009), ~40 hours (Note=Lot 02272015) (JCRB=IFO50073); 36 hours (Note=Lot 041392), ~5 days (Note=Lot 031098), ~2 days (Note=Lot 05192003), ~1.5 days (Note=Lot 05202005), ~34 hours (Note=Lot 06022011), ~38 hours (Note=Lot 04212016) (JCRB=JCRB9008).
CC   HLA typing: A*02:01,29:02; B*07:02,44:02; C*05:01,07:02 (PubMed=31869419).
CC   Omics: DNA methylation analysis.
CC   Omics: Glycosphingolipids analysis.
CC   Omics: Proteome analysis by 2D-DE.
CC   Omics: Proteome analysis by 2D-DE/MS.
CC   Omics: Transcriptome analysis by microarray.
CC   Omics: Transcriptome analysis by RNAseq.
CC   Misspelling: MCR-5; Note=Occasionally.
CC   Discontinued: CLS; 300395; true.
CC   Discontinued: ECACC; 97112601; true.
CC   Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
CC   Cell type: Fibroblast of lung; CL=CL_0002553.
ST   Source(s): AddexBio=T0016002/4922; ATCC=CCL-171; CCRID; JCRB=IFO50073; JCRB=JCRB9008; KCLB=10171; PubMed=11416159; PubMed=21637780; PubMed=25877200; RCB=RCB0211
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D13S317: 11,14
ST   D16S539: 9,11
ST   D18S51: 15,21
ST   D19S433: 14,15
ST   D21S11: 31.2
ST   D2S1338: 20
ST   D3S1358: 15,17
ST   D5S818: 11,12
ST   D7S820: 10,11
ST   D8S1179: 13
ST   FGA: 21,23
ST   Penta D: 12
ST   Penta E: 12,16
ST   TH01: 8
ST   TPOX: 8
ST   vWA: 15
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   14FW
CA   Finite cell line
DT   Created: 04-04-12; Last updated: 02-05-24; Version: 40
RX   CelloPub=CLPUB00391;
RA   Wadman M.;
RT   "The vaccine race: science, politics, and the human costs of defeating
RT   disease.";
RL   (In) ISBN 9780525427537; pp.1-436; Viking; New York (2016).
RX   PubMed=113411; DOI=10.1016/S0092-1157(79)80043-8;
RA   Jacobs J.P., Garrett A.J., Merton R.;
RT   "Characteristics of a serially propagated human diploid cell
RT   designated MRC-9.";
RL   J. Biol. Stand. 7:113-122(1979).
RX   PubMed=211143; DOI=10.1128/jcm.7.4.368-371.1978;
RA   Friedman H.M., Koropchak C.;
RT   "Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of
RT   viruses from clinical specimens.";
RL   J. Clin. Microbiol. 7:368-371(1978).
RX   PubMed=932048; DOI=10.1016/0092-1157(76)90018-4;
RA   Jacobs J.P.;
RT   "The status of human diploid cell strain MRC-5 as an approved
RT   substrate for the production of viral vaccines.";
RL   J. Biol. Stand. 4:97-99(1976).
RX   PubMed=1031681;
RA   Jacobs J.P.;
RT   "Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell
RT   strains.";
RL   Dev. Biol. Stand. 37:155-156(1976).
RX   PubMed=1965304; DOI=10.1002/elps.1150111203;
RA   Celis J.E., Dejgaard K., Madsen P., Leffers H., Gesser B., Honore B.,
RA   Rasmussen H.H., Olsen E., Lauridsen J.B., Ratz G., Mouritzen S.,
RA   Basse B., Hellerup M., Celis A., Puype M., Van Damme J.,
RA   Vandekerckhove J.;
RT   "The MRC-5 human embryonal lung fibroblast two-dimensional gel
RT   cellular protein database: quantitative identification of polypeptides
RT   whose relative abundance differs between quiescent, proliferating and
RT   SV40 transformed cells.";
RL   Electrophoresis 11:1072-1113(1990).
RX   PubMed=3335022;
RA   Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J.,
RA   Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.;
RT   "Feasibility of drug screening with panels of human tumor cell lines
RT   using a microculture tetrazolium assay.";
RL   Cancer Res. 48:589-601(1988).
RX   PubMed=4316953; DOI=10.1038/227168a0;
RA   Jacobs J.P., Jones C.M., Baille J.P.;
RT   "Characteristics of a human diploid cell designated MRC-5.";
RL   Nature 227:168-170(1970).
RX   PubMed=6538202; DOI=10.1083/jcb.98.3.1133;
RA   Azzarone B., Suarez H.G., Mingari M.C., Moretta L., Fauci A.S.;
RT   "4F2 monoclonal antibody recognizes a surface antigen on spread human
RT   fibroblasts of embryonic but not of adult origin.";
RL   J. Cell Biol. 98:1133-1137(1984).
RX   PubMed=6971834; DOI=10.1080/09553008114550251;
RA   Cooke A., Harris W.J.;
RT   "Pyrimidine dimer excision in ageing human cells in tissue culture.";
RL   Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 39:223-226(1981).
RX   PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222;
RA   Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.;
RT   "Cultured cell lines for research on pulmonary physiology available
RT   through the American Type Culture Collection.";
RL   Am. Rev. Respir. Dis. 125:222-232(1982).
RX   PubMed=10752125;
RA   Poljanskaia G.G., Efremova T.N., Sakuta G.A.;
RT   "The effect of mycoplasmal contamination of human embryonic lung cell
RT   line MRC-5 on the karyotypic variability.";
RL   Tsitologiia 42:190-195(2000).
RX   PubMed=11416159; DOI=10.1073/pnas.121616198;
RA   Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G.,
RA   Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R.,
RA   Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.;
RT   "Short tandem repeat profiling provides an international reference
RT   standard for human cell lines.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
RX   PubMed=17395773; DOI=10.1634/stemcells.2006-0504;
RA   Sudo K., Kanno M., Miharada K., Ogawa S., Hiroyama T., Saijo K.,
RA   Nakamura Y.;
RT   "Mesenchymal progenitors able to differentiate into osteogenic,
RT   chondrogenic, and/or adipogenic cells in vitro are present in most
RT   primary fibroblast-like cell populations.";
RL   Stem Cells 25:1610-1617(2007).
RX   PubMed=19657000;
RA   Rubporn A., Srisomsap C., Subhasitanont P., Chokchaichamnankit D.,
RA   Chiablaem K., Svasti J., Sangvanich P.;
RT   "Comparative proteomic analysis of lung cancer cell line and lung
RT   fibroblast cell line.";
RL   Cancer Genomics Proteomics 6:229-237(2009).
RX   PubMed=21637780; DOI=10.1371/journal.pgen.1002085;
RA   Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E.,
RA   Sakaguchi H., Akutsu H., Umezawa A.;
RT   "DNA methylation dynamics in human induced pluripotent stem cells over
RT   time.";
RL   PLoS Genet. 7:E1002085-E1002085(2011).
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M.,
RA   Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S.,
RA   Neve R.M.;
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
RX   PubMed=25903999; DOI=10.1002/biot.201400388;
RA   Genzel Y.;
RT   "Designing cell lines for viral vaccine production: where do we
RT   stand?";
RL   Biotechnol. J. 10:728-740(2015).
RX   PubMed=26477663; DOI=10.1038/srep14988;
RA   Ojima T., Shibata E., Saito S., Toyoda M., Nakajima H.,
RA   Yamazaki-Inoue M., Miyagawa Y., Kiyokawa N., Fujimoto J.-i., Sato T.,
RA   Umezawa A.;
RT   "Glycolipid dynamics in generation and differentiation of induced
RT   pluripotent stem cells.";
RL   Sci. Rep. 5:14988-14988(2015).
RX   PubMed=31869419; DOI=10.1084/jem.20191164;
RA   Lubke M., Spalt S., Kowalewski D.J., Zimmermann C., Bauersfeld L.,
RA   Nelde A., Bichmann L., Marcu A., Peper J.K., Kohlbacher O., Walz J.S.,
RA   Le-Trilling V.T.K., Hengel H., Rammensee H.-G., Stevanovic S.,
RA   Halenius A.;
RT   "Identification of HCMV-derived T cell epitopes in seropositive
RT   individuals through viral deletion models.";
RL   J. Exp. Med. 217:20191164.1-20191164.14(2020).
RX   PubMed=33389257; DOI=10.1007/s10096-020-04106-0;
RA   Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.;
RT   "Culture of SARS-CoV-2 in a panel of laboratory cell lines,
RT   permissivity, and differences in growth profile.";
RL   Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021).