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Cellosaurus 4T1 (CVCL_0125)

[Text version]
Cell line name 4T1
Synonyms 4T1-A; 4T1.0; 4T1/WT
Accession CVCL_0125
Resource Identification Initiative To cite this cell line use: 4T1 (RRID:CVCL_0125)
Comments Characteristics: Metastatic.
Characteristics: Widely used model in cancer research due to its high similarity to human breast cancer. The tumor growth and metastatic spread of 4T1 cells closely mimic the behavior of human breast cancer. The 4T1 cell line is considered an animal model for stage IV human breast cancer, as it is able to spontaneously metastasize in both post-operative and non-surgical models (CLS=300300).
Doubling time: 12.6 hours (ATCC=CRL-2539).
Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
Disease Malignant neoplasms of the mouse mammary gland (NCIt: C21678)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H.
Hierarchy Parent: CVCL_W343 (410.4)
CVCL_QZ56 (4T1-eGFP-Puro)CVCL_QZ57 (4T1-Fluc-Neo)CVCL_QZ58 (4T1-Fluc-Neo/eGFP-Puro)
CVCL_QZ59 (4T1-Fluc-Neo/iRFP-Puro)CVCL_QZ60 (4T1-iRFP-Puro)CVCL_J239 (4T1-Luc [JCRB])
CVCL_C8UY (4T1-Luc [Ubigene])CVCL_A4BM (4T1-Luc2 [ATCC])CVCL_L899 (4T1-luc2 [Caliper])
CVCL_5J28 (4T1-luc2-GFP)CVCL_5J46 (4T1-luc2-tdTomato)CVCL_C8UZ (4T1-mCherry)
CVCL_QZ61 (4T1-mNIS (monoclonal))CVCL_QZ62 (4T1-mNIS-Neo/eGFP-Puro)CVCL_QZ63 (4T1-mNIS-Neo/iRFP-Puro)
CVCL_QZ64 (4T1-mNIS-Puro)CVCL_QZ65 (4T1-mNIS-Puro/Fluc-Neo)CVCL_5I84 (4T1-Red-FLuc)
CVCL_5I85 (4T1-Red-FLuc-GFP)CVCL_HE47 (4T1-S)CVCL_5J45 (4T1-tdTomato)
CVCL_GR31 (4T1.13)CVCL_GR32 (4T1.2)CVCL_B3QZ (4T1.3)
CVCL_C9FJ (4T1/Cas9-hyg)CVCL_JG34 (4T1/CMV-Luc #6)CVCL_C0U6 (4T1/EPB)
CVCL_XG69 (4T1/GFP/FlucII)CVCL_D6AA (HyCyte 4T1 KO-mEhd4)CVCL_D6AB (HyCyte 4T1 KO-mInhba)
CVCL_D6AC (HyCyte 4T1 KO-mUsp22)CVCL_D6AD (HyCyte 4T1 KO-mZdhhc20)
Sex of cell Female
Category Cancer cell line
STR profile Source(s): PubMed=31220119; Ubigene=YC-B004

Mouse STR 1-115,16
Mouse STR 1-217
Mouse STR 2-116,17
Mouse STR 3-214,15
Mouse STR 4-221.3
Mouse STR 5-514
Mouse STR 6-418
Mouse STR 6-712
Mouse STR 7-125.2
Mouse STR 8-113
Mouse STR 11-218,19,20
Mouse STR 12-116
Mouse STR 13-116.2
Mouse STR 15-322.3
Mouse STR 17-215
Mouse STR 18-318,19
Mouse STR 19-213
Mouse STR X-125

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/4T1

PubMed=10411109; DOI=10.1023/A:1006689719505
Lelekakis M., Moseley J.M., Martin T.J., Hards D., Williams E., Ho P., Lowen D., Javni J., Miller F.R., Slavin J., Anderson R.L.
A novel orthotopic model of breast cancer metastasis to bone.
Clin. Exp. Metastasis 17:163-170(1999)

PubMed=26857856; DOI=10.1007/s13577-015-0127-1
Abe H., Wada H., Baghdadi M., Nakanishi S., Usui Y., Tsuchikawa T., Shichinohe T., Hirano S., Seino K.-i.
Identification of a highly immunogenic mouse breast cancer sub cell line, 4T1-S.
Hum. Cell 29:58-66(2016)

PubMed=27177471; DOI=10.1016/j.canlet.2016.05.005
Sasaki S.-i., Baba T., Nishimura T., Hayakawa Y., Hashimoto S.-i., Gotoh N., Mukaida N.
Essential roles of the interaction between cancer cell-derived chemokine, CCL4, and intra-bone CCR5-expressing fibroblasts in breast cancer bone metastasis.
Cancer Lett. 378:23-32(2016)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

Cell line collections (Providers) AddexBio; C0006004/13
ATCC; CRL-2539
BCRJ; 0022
CLS; 300300
KCB; KCB 200967YJ
NCBI_Iran; C604
Ubigene; YC-B004
Cell line databases/resources CLO; CLO_0001401
MCCL; MCC:0000017
CCRID; 6101MOU-CERC000107
Lonza; 481
Anatomy/cell type resources BTO; BTO:0004311
Biological sample resources BioSample; SAMN11397647
CRISP screens repositories BioGRID_ORCS_Cell_line; 1260
Chemistry resources ChEMBL-Cells; CHEMBL3307995
ChEMBL-Targets; CHEMBL612589
PubChem_Cell_line; CVCL_0125
Encyclopedic resources Wikidata; Q42802273
Experimental variables resources EFO; EFO_0002037
Gene expression databases GEO; GSM1529765
GEO; GSM1529766
GEO; GSM1529767
GEO; GSM1847461
GEO; GSM1847462
Proteomic databases PRIDE; PXD013948
PRIDE; PXD016112
PRIDE; PXD021413
PRIDE; PXD024762
PRIDE; PXD027822
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number32