Cellosaurus 4T1-SIINFEKL/H-2Kb (CVCL_E7FZ)
| Cell line name | 4T1-SIINFEKL/H-2Kb |
|---|---|
| Accession | CVCL_E7FZ |
| Resource Identification Initiative | To cite this cell line use: 4T1-SIINFEKL/H-2Kb (RRID:CVCL_E7FZ) |
| Comments | Characteristics: Enables the large-scale and continuous batch production of soluble, fully folded and epitope-defined MHC class I molecules. Engineered to secrete peptide/MHC complexes that can be selectively purified in bulk using affinity chromatography, biotinylated, multimerized (via streptavidin) and the resulting reagent incorporated into immunologically relevant assays for detection of antigen-specific T-cells (PubMed=30347189). Characteristics: Using a sleeping beauty transposon system a SIINFEKL-linker-B2m-linker-H2K(b)-AviTag construct was integrated in the parent cell line (PubMed=30347189). Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:88127; B2m. Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:95904; H2-K1. Genetic integration: Method=Sleeping Beauty transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911. |
| Disease | Malignant neoplasms of the mouse mammary gland (NCIt: C21678) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/cfC3H. |
| Hierarchy | Parent: CVCL_0125 (4T1) |
| Sex of cell | Female |
| Category | Cancer cell line |
| Publications | PubMed=30347189; DOI=10.1016/j.jim.2018.10.006; PMCID=PMC6322931 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |