ID   4T1-SIINFEKL/H-2Kb
AC   CVCL_E7FZ
RX   PubMed=30347189;
CC   Characteristics: Enables the large-scale and continuous batch production of soluble, fully folded and epitope-defined MHC class I molecules. Engineered to secrete peptide/MHC complexes that can be selectively purified in bulk using affinity chromatography, biotinylated, multimerized (via streptavidin) and the resulting reagent incorporated into immunologically relevant assays for detection of antigen-specific T-cells (PubMed=30347189).
CC   Characteristics: Using a sleeping beauty transposon system a SIINFEKL-linker-B2m-linker-H2K(b)-AviTag construct was integrated in the parent cell line (PubMed=30347189).
CC   Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:88127; B2m.
CC   Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:95904; H2-K1.
CC   Genetic integration: Method=Sleeping Beauty transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Breed/subspecies: BALB/cfC3H.
DI   NCIt; C21678; Malignant neoplasms of the mouse mammary gland
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0125 ! 4T1
SX   Female
CA   Cancer cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=30347189; DOI=10.1016/j.jim.2018.10.006; PMCID=PMC6322931;
RA   Wooster A.L., Anderson T.S., Lowe D.B.;
RT   "Expression and characterization of soluble epitope-defined major
RT   histocompatibility complex (MHC) from stable eukaryotic cell lines.";
RL   J. Immunol. Methods 464:22-30(2019).
//