Cellosaurus cell line THP1-Dual (CVCL

Cellosaurus THP1-Dual (CVCL_X599)

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Cell line name

THP1-Dual

Accession

CVCL_X599

Resource Identification Initiative

To cite this cell line use: THP1-Dual (RRID:CVCL_X599)

Comments

Population: Japanese.
Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites and 3 C-Rel binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE) (InvivoGen=thpd-nfis).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection; Gene=Synthetic construct Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.

Sequence variations

Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).

Disease

Childhood acute monocytic leukemia (NCIt: C9163)
Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0006 (THP-1)
Children:

CVCL_E8FA (THP1-Dual hTLR3)	CVCL_E8FB (THP1-Dual hTLR7)	CVCL_E8FC (THP1-Dual hTLR8)
CVCL_E8FD (THP1-Dual hTLR9)	CVCL_A8AI (THP1-Dual KO-cGAS)	CVCL_E8F4 (THP1-Dual KO-DNase2)
CVCL_A8AU (THP1-Dual KO-IFI16)	CVCL_A8AL (THP1-Dual KO-IFNAR2)	CVCL_A8AZ (THP1-Dual KO-IKKepsilon)
CVCL_E8F5 (THP1-Dual KO-IRF1)	CVCL_A8AV (THP1-Dual KO-IRF3)	CVCL_E8F6 (THP1-Dual KO-IRF5)
CVCL_E8F7 (THP1-Dual KO-IRF7)	CVCL_A8BA (THP1-Dual KO-MAVS)	CVCL_A8BB (THP1-Dual KO-MDA5)
CVCL_5J54 (THP1-Dual KO-MyD)	CVCL_A8BC (THP1-Dual KO-RIG-I)	CVCL_E8F8 (THP1-Dual KO-SAMHD1)
CVCL_A8AH (THP1-Dual KO-STING)	CVCL_A8AY (THP1-Dual KO-TBK1)	CVCL_A8AW (THP1-Dual KO-TLR2)
CVCL_A8AX (THP1-Dual KO-TLR4)	CVCL_A8BW (THP1-Dual KO-TLR8)	CVCL_A8AJ (THP1-Dual KO-TREX1)
CVCL_A8AM (THP1-Dual KO-TRIF)	CVCL_E8FF (THP1-Dual MD2-CD14 KO-TLR4)	CVCL_E8FE (THP1-Dual MD2-CD14-TLR4)

Sex of cell

Male

Age at sampling