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Cellosaurus CHO MGAT1- (CVCL_VP59)

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Cell line name CHO MGAT1-
Synonyms MGAT1- CHO; MGAT1- CHO 3.4F10; 3.4F10; 3.4F10 MGAT1- CHO; CHO.K1.MGAT1-
Accession CVCL_VP59
Resource Identification Initiative To cite this cell line use: CHO MGAT1- (RRID:CVCL_VP59)
Comments Characteristics: This cell line can only produce N-linked carbohydrates with early oligomannose glycans (Man5-9).
Doubling time: 20.7 hours (PubMed=30157178).
Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7183 (CHO-S)
Children:
CVCL_A9NX (CHO C1s-/- MGAT1-)CVCL_A9NZ (CHO C1s-/- MGAT1- BaL-rgp120)CVCL_A0BY (CHO MGAT1- A244_N332-rgp120 5F)
CVCL_A0BT (CHO MGAT1- TZ97008-rgp120 2D7)CVCL_A0BU (CHO MGAT1- TZ97008-rgp120 3E5)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=30071025; DOI=10.1371/journal.pone.0197656; PMCID=PMC6071959
Sara M. O'Rourke, Gabriel Byrne, Gwen P. Tatsuno, Meredith Wright, Bin Yu, Kathryn A. Mesa, Rachel C. Doran, David L. Alexander, Phillip W. Berman;
Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production.
PLoS ONE 13:e0197656.1-e0197656.22(2018)

PubMed=30157178; DOI=10.1371/journal.pbio.2005817; PMCID=PMC6133382
Gabriel Byrne, Sara M. O'Rourke, David L. Alexander, Bin Yu, Rachel C. Doran, Meredith Wright, Qiu-Shi Chen, Parastoo Azadi, Phillip W. Berman;
CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.
PLoS Biol. 16:e2005817.1-e2005817.23(2018)

PubMed=31087560; DOI=10.1002/bit.27016; PMCID=PMC6675663
Sophia W. Li, Bin Yu, Gabriel Byrne, Meredith Wright, Sara M. O'Rourke, Kathryn A. Mesa, Phillip W. Berman;
Identification and CRISPR/Cas9 inactivation of the C1s protease responsible for proteolysis of recombinant proteins produced in CHO cells.
Biotechnol. Bioeng. 116:2130-2145(2019)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-13576
Encyclopedic resources Wikidata; Q93451231
Entry history
Entry creation07-Sep-2018
Last entry update27-Nov-2025
Version number10