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Cellosaurus CHO MGAT1- (CVCL_VP59)

[Text version]
Cell line name CHO MGAT1-
Synonyms MGAT1- CHO; MGAT1- CHO 3.4F10; 3.4F10; CHO.K1.MGAT1-
Accession CVCL_VP59
Resource Identification Initiative To cite this cell line use: CHO MGAT1- (RRID:CVCL_VP59)
Comments Characteristics: This cell line can only produce N-linked carbohydrates with early oligomannose glycans (Man5-9).
Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7183 (CHO-S)
Children:
CVCL_A9NX (CHO C1s-/- MGAT1-)CVCL_A9NZ (CHO C1s-/- MGAT1- BaL-rgp120)CVCL_A0BY (CHO MGAT1- A244_N332-rgp120 5F)
CVCL_A0BT (CHO MGAT1- TZ97008-rgp120 2D7)CVCL_A0BU (CHO MGAT1- TZ97008-rgp120 3E5)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=30157178; DOI=10.1371/journal.pbio.2005817
Byrne G., O'Rourke S.M., Alexander D.L., Yu B., Doran R.C., Wright M., Chen Q.-S., Azadi P., Berman P.W.
CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation.
PLoS Biol. 16:e2005817.1-e2005817.23(2018)

PubMed=30071025; DOI=10.1371/journal.pone.0197656
O'Rourke S.M., Byrne G., Tatsuno G.P., Wright M., Yu B., Mesa K.A., Doran R.C., Alexander D.L., Berman P.W.
Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production.
PLoS ONE 13:E0197656-E0197656(2018)

PubMed=31087560; DOI=10.1002/bit.27016
Li S.W., Yu B., Byrne G., Wright M., O'Rourke S.M., Mesa K.A., Berman P.W.
Identification and CRISPR/Cas9 inactivation of the C1s protease responsible for proteolysis of recombinant proteins produced in CHO cells.
Biotechnol. Bioeng. 116:2130-2145(2019)

Cross-references
Cell line collections (Providers) HIVReagentProgram; ARP-13576
Encyclopedic resources Wikidata; Q93451231
Entry history
Entry creation07-Sep-2018
Last entry update30-Jan-2024
Version number7