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Cellosaurus CHO C1s-/- MGAT1- (CVCL_A9NX)

[Text version]
Cell line name CHO C1s-/- MGAT1-
Synonyms C1s-/- MGAT1- CHO; CHO.K1.MGAT1-Prot-; CHO-s-MGAT1-Protease-
Accession CVCL_A9NX
Resource Identification Initiative To cite this cell line use: CHO C1s-/- MGAT1- (RRID:CVCL_A9NX)
Comments Group: Serum/protein free medium cell line.
Characteristics: This cell line minimizes proteolysis of clade B HIV-1 envelope proteins commonly observed in the production of monomeric gp120 and gp140 as well as gp140 trimers.
Characteristics: This cell line can only produce N-linked carbohydrates with early oligomannose glycans (Man5-9).
Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GUR0; C1s.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_VP59 (CHO MGAT1-)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=31087560; DOI=10.1002/bit.27016
Li S.W., Yu B., Byrne G., Wright M., O'Rourke S.M., Mesa K.A., Berman P.W.
Identification and CRISPR/Cas9 inactivation of the C1s protease responsible for proteolysis of recombinant proteins produced in CHO cells.
Biotechnol. Bioeng. 116:2130-2145(2019)

Cross-references
Cell line collections (Providers) HIVReagentProgram; ARP-13574
Encyclopedic resources Wikidata; Q108820026
Entry history
Entry creation23-Sep-2021
Last entry update30-Jan-2024
Version number5