ID   CHO MGAT1-
AC   CVCL_VP59
SY   MGAT1- CHO; MGAT1- CHO 3.4F10; 3.4F10; CHO.K1.MGAT1-
DR   HIVReagentProgram; ARP-13576
DR   Wikidata; Q93451231
RX   PubMed=30157178;
RX   PubMed=30071025;
RX   PubMed=31087560;
CC   Characteristics: This cell line can only produce N-linked carbohydrates with early oligomannose glycans (Man5-9).
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_7183 ! CHO-S
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 07-09-18; Last updated: 30-01-24; Version: 7
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RX   PubMed=30157178; DOI=10.1371/journal.pbio.2005817;
RA   Byrne G., O'Rourke S.M., Alexander D.L., Yu B., Doran R.C., Wright M.,
RA   Chen Q.-S., Azadi P., Berman P.W.;
RT   "CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO
RT   cell line to control HIV-1 vaccine glycosylation.";
RL   PLoS Biol. 16:e2005817.1-e2005817.23(2018).
//
RX   PubMed=30071025; DOI=10.1371/journal.pone.0197656;
RA   O'Rourke S.M., Byrne G., Tatsuno G.P., Wright M., Yu B., Mesa K.A.,
RA   Doran R.C., Alexander D.L., Berman P.W.;
RT   "Robotic selection for the rapid development of stable CHO cell lines
RT   for HIV vaccine production.";
RL   PLoS ONE 13:E0197656-E0197656(2018).
//
RX   PubMed=31087560; DOI=10.1002/bit.27016;
RA   Li S.W., Yu B., Byrne G., Wright M., O'Rourke S.M., Mesa K.A.,
RA   Berman P.W.;
RT   "Identification and CRISPR/Cas9 inactivation of the C1s protease
RT   responsible for proteolysis of recombinant proteins produced in CHO
RT   cells.";
RL   Biotechnol. Bioeng. 116:2130-2145(2019).
//