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Cellosaurus CHO MGAT1- A244_N332-rgp120 5F (CVCL_A0BY)

[Text version]
Cell line name CHO MGAT1- A244_N332-rgp120 5F
Synonyms 6.4D
Accession CVCL_A0BY
Resource Identification Initiative To cite this cell line use: CHO MGAT1- A244_N332-rgp120 5F (RRID:CVCL_A0BY)
Comments Characteristics: Produces a gp120 from a modified A244 isolate (A244_N332-rgp120) where a glycosylation was moved from Asn-334 to Asn-332. It is enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD) (PubMed=30071025).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_VP59 (CHO MGAT1-)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=30071025; DOI=10.1371/journal.pone.0197656; PMCID=PMC6071959
Sara M. O'Rourke, Gabriel Byrne, Gwen P. Tatsuno, Meredith Wright, Bin Yu, Kathryn A. Mesa, Rachel C. Doran, David L. Alexander, Phillip W. Berman;
Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production.
PLoS ONE 13:e0197656.1-e0197656.22(2018)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-13577
Encyclopedic resources Wikidata; Q108820031
Entry history
Entry creation23-Sep-2021
Last entry update14-Aug-2025
Version number7