ID   CHO MGAT1- A244_N332-rgp120 5F
AC   CVCL_A0BY
SY   6.4D
DR   BEI_Resources; ARP-13577
DR   Wikidata; Q108820031
RX   PubMed=30071025;
CC   Characteristics: Produces a gp120 from a modified A244 isolate (A244_N332-rgp120) where a glycosylation was moved from Asn-334 to Asn-332. It is enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD) (PubMed=30071025).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_VP59 ! CHO MGAT1-
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 23-09-21; Last updated: 10-09-24; Version: 6
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RX   PubMed=30071025; DOI=10.1371/journal.pone.0197656; PMCID=PMC6071959;
RA   O'Rourke S.M., Byrne G., Tatsuno G.P., Wright M., Yu B., Mesa K.A.,
RA   Doran R.C., Alexander D.L., Berman P.W.;
RT   "Robotic selection for the rapid development of stable CHO cell lines
RT   for HIV vaccine production.";
RL   PLoS ONE 13:e0197656.1-e0197656.22(2018).
//