Cellosaurus cell line H9 NR4A2-eGFP (CVCL

Cellosaurus H9 NR4A2-eGFP (CVCL_E7GG)

[Text version]

Cell line name

H9 NR4A2-eGFP

Synonyms

H9 NURR1-GFP reporter; H9-Nurr1::H2B-GFP

Accession

CVCL_E7GG

Resource Identification Initiative

To cite this cell line use: H9 NR4A2-eGFP (RRID:CVCL_E7GG)

Comments

Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (PubMed=31543366).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_9773 (WA09)
Children:

CVCL_E7GJ (H9 NR4A2-eGFP iCas9)

CVCL_E7GH (H9 NR4A2-eGFP SATB1 KO)

CVCL_E7GI (H9 NR4A2-eGFP TNF KO)

Sex of cell

Female

Age at sampling

Blastocyst stage

Category

Embryonic stem cell

Publications

PubMed=31543366; DOI=10.1016/j.stem.2019.08.013; PMCID=PMC7493192
Markus Riessland, Benjamin Kolisnyk, Tae Wan Kim, Jia Cheng, Jason Ni, Jordan A. Pearson, Emily Jean-Young Park, Kevin Dam, Devrim Acehan, Lavoisier S. Ramos-Espiritu, Wei Wang ...Show all 17 authors... , Jack Zhang, Jae-Won Shim, Gabriele Ciceri, Lars Brichta, Lorenz P. Studer, Paul Greengard; Show fewer authors
Loss of SATB1 induces p21-dependent cellular senescence in post-mitotic dopaminergic neurons.
Cell Stem Cell 25:514-530.e8(2019)

PubMed=38866017; DOI=10.1016/j.cell.2024.05.030; PMCID=PMC11641762
Tae Wan Kim, So Yeon Koo, Markus Riessland, Fayzan F. Chaudhry, Benjamin Kolisnyk, Hyein Sophia Cho, Marco Vincenzo Russo, Nathalie Saurat, Sanjoy Mehta, Ralph Garippa, Doron Betel, Lorenz P. Studer;
TNF-NF-kappaB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons.
Cell 187:3671-3689.e23(2024)

Entry history

Entry creation

10-Apr-2025

Last entry update

14-Aug-2025

Version number