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Cellosaurus H9 NR4A2-eGFP SATB1 KO (CVCL_E7GH)

[Text version]
Cell line name H9 NR4A2-eGFP SATB1 KO
Synonyms H9 NURR1-GFP reporter SATB1KO
Accession CVCL_E7GH
Resource Identification Initiative To cite this cell line use: H9 NR4A2-eGFP SATB1 KO (RRID:CVCL_E7GH)
Comments Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:10541; SATB1.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E7GG (H9 NR4A2-eGFP)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=31543366; DOI=10.1016/j.stem.2019.08.013; PMCID=PMC7493192
Riessland M., Kolisnyk B., Kim T.W., Cheng J., Ni J., Pearson J.A., Park E.J.-Y., Dam K., Acehan D., Ramos-Espiritu L.S., Wang W., Zhang J., Shim J.-W., Ciceri G., Brichta L., Studer L.P., Greengard P.
Loss of SATB1 induces p21-dependent cellular senescence in post-mitotic dopaminergic neurons.
Cell Stem Cell 25:514-530.e8(2019)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1