ID   H9 NR4A2-eGFP SATB1 KO
AC   CVCL_E7GH
SY   H9 NURR1-GFP reporter SATB1KO
RX   PubMed=31543366;
CC   Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:10541; SATB1.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E7GG ! H9 NR4A2-eGFP
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
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RX   PubMed=31543366; DOI=10.1016/j.stem.2019.08.013; PMCID=PMC7493192;
RA   Riessland M., Kolisnyk B., Kim T.W., Cheng J., Ni J., Pearson J.A.,
RA   Park E.J.-Y., Dam K., Acehan D., Ramos-Espiritu L.S., Wang W., Zhang J.,
RA   Shim J.-W., Ciceri G., Brichta L., Studer L.P., Greengard P.;
RT   "Loss of SATB1 induces p21-dependent cellular senescence in
RT   post-mitotic dopaminergic neurons.";
RL   Cell Stem Cell 25:514-530.e8(2019).
//