Cellosaurus cell line H9 NR4A2-eGFP iCas9 (CVCL

Cellosaurus H9 NR4A2-eGFP iCas9 (CVCL_E7GJ)

[Text version]

Cell line name

H9 NR4A2-eGFP iCas9

Synonyms

H9-Nurr1::H2B-GFP-iCas9; H9 iCas9/NURR1::GFP

Accession

CVCL_E7GJ

Resource Identification Initiative

To cite this cell line use: H9 NR4A2-eGFP iCas9 (RRID:CVCL_E7GJ)

Comments

Characteristics: Cell line with robust Dox-inducible expression of Cas9 (PubMed=38866017).
Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line).
Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (PubMed=38866017).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_E7GG (H9 NR4A2-eGFP)
Children:

CVCL_E7GK (H9 NR4A2-eGFP iCas9 LRRK2(G2019S/G2019S))

Sex of cell

Female

Age at sampling

Blastocyst stage