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Cellosaurus H9 NR4A2-eGFP iCas9 (CVCL_E7GJ)

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Cell line name H9 NR4A2-eGFP iCas9
Synonyms H9-Nurr1::H2B-GFP-iCas9; H9 iCas9/NURR1::GFP
Accession CVCL_E7GJ
Resource Identification Initiative To cite this cell line use: H9 NR4A2-eGFP iCas9 (RRID:CVCL_E7GJ)
Comments Characteristics: Cell line with robust Dox-inducible expression of Cas9 (PubMed=38866017).
Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line).
Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (PubMed=38866017).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E7GG (H9 NR4A2-eGFP)
Children:
CVCL_E7GK (H9 NR4A2-eGFP iCas9 LRRK2(G2019S/G2019S))
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=38866017; DOI=10.1016/j.cell.2024.05.030; PMCID=PMC11641762
Kim T.W., Koo S.Y., Riessland M., Chaudhry F.F., Kolisnyk B., Cho H.S., Russo M.V., Saurat N., Mehta S., Garippa R., Betel D., Studer L.P.
TNF-NF-kappaB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons.
Cell 187:3671-3689.e23(2024)

PubMed=38917806; DOI=10.1016/j.stem.2024.06.001; PMCID=PMC11405001
Saurat N., Minotti A.P., Rahman M.T., Sikder T., Zhang C., Cornacchia D., Jungverdorben J., Ciceri G., Betel D., Studer L.P.
Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration.
Cell Stem Cell 31:1162-1174.e8(2024)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1