Cellosaurus H9 NR4A2-eGFP iCas9 (CVCL_E7GJ)
Cell line name | H9 NR4A2-eGFP iCas9 | |
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Synonyms | H9-Nurr1::H2B-GFP-iCas9; H9 iCas9/NURR1::GFP | |
Accession | CVCL_E7GJ | |
Resource Identification Initiative | To cite this cell line use: H9 NR4A2-eGFP iCas9 (RRID:CVCL_E7GJ) | |
Comments | Characteristics: Cell line with robust Dox-inducible expression of Cas9 (PubMed=38866017). Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line). Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (PubMed=38866017). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. | |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | |
Hierarchy | Parent: CVCL_E7GG (H9 NR4A2-eGFP) Children:
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Sex of cell | Female | |
Age at sampling | Blastocyst stage | |
Category | Embryonic stem cell | |
Publications | PubMed=38866017; DOI=10.1016/j.cell.2024.05.030; PMCID=PMC11641762 PubMed=38917806; DOI=10.1016/j.stem.2024.06.001; PMCID=PMC11405001 | |
Entry history | ||
Entry creation | 10-Apr-2025 | |
Last entry update | 10-Apr-2025 | |
Version number | 1 |