Cellosaurus H9 NR4A2-eGFP iCas9 LRRK2(G2019S/G2019S) (CVCL_E7GK)
| Cell line name | H9 NR4A2-eGFP iCas9 LRRK2(G2019S/G2019S) |
|---|---|
| Synonyms | H9-Nurr1::H2B-GFP-iCas9-LRRK2G2019S/G2019S |
| Accession | CVCL_E7GK |
| Resource Identification Initiative | To cite this cell line use: H9 NR4A2-eGFP iCas9 LRRK2(G2019S/G2019S) (RRID:CVCL_E7GK) |
| Comments | Characteristics: Cell line with robust Dox-inducible expression of Cas9 and an edited homozygous LRRK2 (G2019S) mutation (PubMed=38917806). Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line). Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (from parent cell line). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Sequence variations |
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| Disease | Parkinson disease 8, autosomal dominant (NCIt: C198605) Hereditary late-onset Parkinson disease (ORDO: Orphanet_411602) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_E7GJ (H9 NR4A2-eGFP iCas9) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=38917806; DOI=10.1016/j.stem.2024.06.001; PMCID=PMC11405001 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |