Cellosaurus H9 NR4A2-eGFP TNF KO (CVCL_E7GI)
| Cell line name | H9 NR4A2-eGFP TNF KO |
|---|---|
| Synonyms | H9 TNFa KO/NURR1-GFP |
| Accession | CVCL_E7GI |
| Resource Identification Initiative | To cite this cell line use: H9 NR4A2-eGFP TNF KO (RRID:CVCL_E7GI) |
| Comments | Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:11892; TNF. Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_E7GG (H9 NR4A2-eGFP) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=38866017; DOI=10.1016/j.cell.2024.05.030; PMCID=PMC11641762 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |