ID   H9 NR4A2-eGFP TNF KO
AC   CVCL_E7GI
SY   H9 TNFa KO/NURR1-GFP
RX   PubMed=38866017;
CC   Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:11892; TNF.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E7GG ! H9 NR4A2-eGFP
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
//
RX   PubMed=38866017; DOI=10.1016/j.cell.2024.05.030; PMCID=PMC11641762;
RA   Kim T.W., Koo S.Y., Riessland M., Chaudhry F.F., Kolisnyk B., Cho H.S.,
RA   Russo M.V., Saurat N., Mehta S., Garippa R., Betel D., Studer L.P.;
RT   "TNF-NF-kappaB-p53 axis restricts in vivo survival of hPSC-derived
RT   dopamine neurons.";
RL   Cell 187:3671-3689.e23(2024).
//