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Cellosaurus WA07 (CVCL_9772)

[Text version]
Cell line name WA07
Synonyms WA 07; WA-07; WA7; H7; H7 GE07; H7-hESC; ES-WA7; WAe002-A; WICELLe002-A; WAe007-A; WA07-PCBC; PCBC02hse2014030501; SC14-066
Accession CVCL_9772
Resource Identification Initiative To cite this cell line use: WA07 (RRID:CVCL_9772)
Comments Part of: ENCODE project common cell types; tier 3.
Part of: NHBLI Progenitor Cell Biology Consortium (PCBC) collection.
From: University of Wisconsin; Madison; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0061.
Registration: Swiss research registry; BAG-hES-IMP-0034.
Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18/SCSC11-18.
Omics: Genomics; ChIP-seq; CTCF.
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K36me3.
Omics: Genomics; ChIP-seq; H3K4me1.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; ChIP-seq; H3K9ac.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics.
Omics: Proteomics; PTM; Phosphorylation.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Transcriptomics; Serial analysis of gene expression (SAGE).
Omics: Variations; SNP array analysis.
Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
Donor information: Embryo is sibling to those giving rise to WA13 (Cellosaurus=CVCL_9774) and WA14 (Cellosaurus=CVCL_9775).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
HLA typing Source: PubMed=16919167
Class I
HLA-AA*02:25
HLA-BB*18,35
HLA-CC*04,12
Class II
HLA-DQDQB1*02,03
HLA-DRDRB1*07,11
DRB3*02
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_S798 (H7.s14)CVCL_S799 (H7.s6)CVCL_S800 (H7.s9)
CVCL_E9DF (H7-FOXA2-GFP)CVCL_A5NJ (H7-H2B-RFP)CVCL_A5LL (H7-YAP1)
CVCL_A5LK (H7v1,17q,i20)CVCL_A5KR (H7v1q)CVCL_A5KS (H7v17q)
CVCL_A5KT (H7v20q)CVCL_A8WK (WAe007-A-1)CVCL_C4MS (WAe007-A-2)
CVCL_D0PI (WAe007-A-3)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=16919167; WiCell=wa07

Markers:
AmelogeninX
CSF1PO12
D5S81811,13
D7S82010,11
D13S31711,12
D16S53912,13
TH016
TPOX8,11
vWA14,15

Run an STR similarity search on this cell line
Web pages Info; -; -; https://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/H7-hESC_StamAndCrawford_protocol.pdf
Info; StemCellDB; -; https://stemcelldb.nih.gov/cellLineDetails/Agilent/3
Publications

PubMed=9804556; DOI=10.1126/science.282.5391.1145
James Alexander Thomson, Joseph Itskovitz-Eldor, Sander S. Shapiro, Michelle A. Waknitz, Jennifer J. Swiergiel, Vivienne S. Marshall, Jeffrey M. Jones;
Embryonic stem cell lines derived from human blastocysts.
Science 282:1145-1147(1998)

PubMed=14745950; DOI=10.1002/dvdy.10431
Melissa K. Carpenter, Elen S. Rosler, Gregory J. Fisk, Ralph Brandenberger, Ximena Ares, Takumi Miura, Mary Theresa Lucero, Mahendra Surendra Rao;
Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
Dev. Dyn. 229:243-258(2004)

PubMed=15345125; DOI=10.1089/scd.2004.13.325
Jonathan S. Draper, Harry D. Moore, Ludmila N. Ruban, Paul J. Gokhale, Peter W. Andrews;
Culture and characterization of human embryonic stem cells.
Stem Cells Dev. 13:325-336(2004)

PubMed=16388305; DOI=10.1038/nbt1177
Tenneille E. Ludwig, Mark E. Levenstein, Jeffrey M. Jones, W. Travis Berggren, Erika R. Mitchen, Jennifer L. Frane, Leann J. Crandall, Christine A. Daigh, Kevin R. Conard, Marian S. Piekarczyk, Rachel A. Llanas, James Alexander Thomson;
Derivation of human embryonic stem cells in defined conditions.
Nat. Biotechnol. 24:185-187(2006)

PubMed=16919167; DOI=10.1186/1741-7007-4-28; PMCID=PMC1601965
Richard Josephson, Gregory R. Sykes, Ying Liu, Carol J. Ording, Wei-Ning Xu, Xian-Min Zeng, Soojung Shin, Jeanne Frances Loring, Anirban Maitra, Mahendra Surendra Rao, Jonathan M. Auerbach;
A molecular scheme for improved characterization of human embryonic stem cell lines.
BMC Biol. 4:28.1-28.13(2006)

PubMed=17287758; DOI=10.1038/nbt1285
Duncan E.C. Baker, Neil J. Harrison, Edna Maltby, Kath Smith, Harry D. Moore, Pamela Jean Shaw, Paul R. Heath, Hazel Holden, Peter W. Andrews;
Adaptation to culture of human embryonic stem cells and oncogenesis in vivo.
Nat. Biotechnol. 25:207-215(2007)

PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113; PMCID=PMC2394759
Martin Hirst, Allen Delaney, Sean A. Rogers, Angelique Schnerch, Deryck R. Persaud, Michael D. O'Connor, Thomas Zeng, Michelle Moksa, Keith Fichter, Diana Mah, Anne Go ...Show all 32 authors... , Ryan D. Morin, Agnes Baross, Yong-Jun Zhao, Jaswinder Khattra, Anna-Liisa Prabhu, Pawan Pandoh, Helen McDonald, Jennifer K. Asano, Noreen Dhalla, Kevin Ma, Stephanie Lee, Adrian Ally, Neil Chahal, Stephanie Menzies, Asim Siddiqui, Robert A. Holt, Steven J.M. Jones, Daniela S. Gerhard, James Alexander Thomson, Constance Jean Eaves, Marco Antonio Marra; Show fewer authors
LongSAGE profiling of nine human embryonic stem cell lines.
Genome Biol. 8:R113.1-R113.12(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W. Andrews, Gemma Beighton, Paul A. Bello, Nissim Benvenisty, Lorraine S. Berry, Simon Bevan, Barak Blum ...Show all 85 authors... , Justin Brooking, Kevin G. Chen, Andre B.-H. Choo, Gary A. Churchill, Marie Corbel, Ivan Damjanov, Jonathan S. Draper, Petr Dvorak, Katarina Emanuelsson, Roland A. Fleck, Angela Ford, Karin Gertow, Marina Gertsenstein, Paul J. Gokhale, Rebecca S. Hamilton, Ales Hampl, Lyn E. Healy, Outi Hovatta, Johan Hyllner, Marta P. Imreh, Joseph Itskovitz-Eldor, Jamie Jackson, Jacqueline L. Johnson, Mark Jones, Kehkooi Kee, Benjamin L. King, Barbara B. Knowles, Majlinda Lako, Franck Lebrin, Barbara S. Mallon, Daisy Manning, Yoav Mayshar, Ronald Davies Graham McKay, Anna E. Michalska, Milla Mikkola, Maria Mileikovsky, Stephen Lewis Minger, Harry D. Moore, Christine L. Mummery, Andras Nagy, Norio Nakatsuji, Carmel M. O'Brien, Steve Kah-Weng Oh, Cia Olsson, Timo Otonkoski, Kye-Yoon Park, Robert Passier, Hema Patel, Minal J. Patel, Roger Arnold Pedersen, Martin F. Pera, Marian S. Piekarczyk, Renee A. Reijo Pera, Benjamin E. Reubinoff, Allan J. Robins, Janet Rossant, Peter Rugg-Gunn, Thomas C. Schulz, Henrik Semb, Eric S. Sherrer, Henrike Siemen, Glyn N. Stacey, Miodrag Stojkovic, Hirofumi Suemori, Jin Szatkiewicz, Tikva Tako Turetsky, Timo Tuuri, Stieneke van den Brink, Kristina Vintersten, Sanna Vuoristo, Dorien Ward-van Oostwaard, Thomas A. Weaver, Lesley A. Young, Wei-Dong Zhang; Show fewer authors
International Stem Cell Initiative
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032; PMCID=PMC3063454
Christoph Bock, Evangelos Kiskinis, Griet Verstappen, Hong-Cang Gu, Gabriella L. Boulting, Zachary D. Smith, Michael Johannes Ziller, Gist Fralley Croft, Mackenzie Weygandt Amoroso, Derek H. Oakley, Andreas Gnirke ...Show all 13 authors... , Kevin Carl Eggan, Alexander Meissner; Show fewer authors
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=21983960; DOI=10.1038/nmeth.1699; PMCID=PMC3432645
Douglas H. Phanstiel, Justin Brumbaugh, Craig D. Wenger, Shu-Lan Tian, Mitchell D. Probasco, Derek J. Bailey, Danielle L. Swaney, Mark A. Tervo, Jennifer M. Bolin, Victor Ruotti, Ron Stewart ...Show all 13 authors... , James Alexander Thomson, Joshua J. Coon; Show fewer authors
Proteomic and phosphoproteomic comparison of human ES and iPS cells.
Nat. Methods 8:821-827(2011)

PubMed=22315618; DOI=10.1155/2012/875059; PMCID=PMC3270482
Mari Pekkanen-Mattila, Marisa Ojala, Erja Kerkela, Kristiina Rajala, Heli Skottman, Katriina Aalto-Setala;
The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
Stem Cells Int. 2012:875059.1-875059.10(2012)

PubMed=23117585; DOI=10.1016/j.scr.2012.09.002; PMCID=PMC3590800
Barbara S. Mallon, Joshua G. Chenoweth, Kory R. Johnson, Rebecca S. Hamilton, Paul J. Tesar, Amarendra S. Yavatkar, Leonard J. Tyson, Kyeyoon Park, Kevin G. Chen, Yang-Cheng Fann, Ronald Davies Graham McKay;
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
Stem Cell Res. 10:57-66(2013)

PubMed=23341440; DOI=10.5966/sctm.2012-0047; PMCID=PMC3659749
Sanna Toivonen, Marisa Ojala, Anu Hyysalo, Tanja Ilmarinen, Kristiina Rajala, Mari Pekkanen-Mattila, Riikka Aanismaa, Karolina Lundin, Jaan Palgi, Jere Weltner, Ras Trokovic ...Show all 16 authors... , Olli Silvennoinen, Heli Skottman, Susanna Narkilahti, Katriina Aalto-Setala, Timo Otonkoski; Show fewer authors
Comparative analysis of targeted differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines.
Stem Cells Transl. Med. 2:83-93(2013)

PubMed=27293150; DOI=10.1016/j.stemcr.2016.05.006; PMCID=PMC4944587
Nathan Salomonis, Phillip J. Dexheimer, Larsson Omberg, Robin Schroll, Stacy Bush, Jeffrey S. Huo, Lynn M. Schriml, Shannan Ho Sui, Mehdi Keddache, Christopher N. Mayhew, Shiva Kumar Shanmukhappa ...Show all 23 authors... , James M. Wells, Kenneth Daily, Shane Hubler, Yu-Liang Wang, Elias Thomas Zambidis, Adam A. Margolin, Winston Alexander Hide, Antonis K. Hatzopoulos, Punam Malik, Jose A. Cancelas, Bruce J. Aronow, Carolyn Lutzko; Show fewer authors
Integrated genomic analysis of diverse induced pluripotent stem cells from the Progenitor Cell Biology Consortium.
Stem Cell Reports 7:110-125(2016)

PubMed=27688775; DOI=10.1155/2016/3826249; PMCID=PMC5023833
Pratik K. Nagaria, Carine Robert, Tea Soon Park, Jeffrey S. Huo, Elias Thomas Zambidis, Feyruz V. Rassool;
High-fidelity reprogrammed human IPSCs have a high efficacy of DNA repair and resemble hESCs in their MYC transcriptional signature.
Stem Cells Int. 2016:3826249.1-3826249.14(2016)

PubMed=28445466; DOI=10.1038/nature22312; PMCID=PMC5427175
Florian T. Merkle, Sulagna Ghosh, Nolan Kamitaki, Jana Mitchell, Yishai Avior, Curtis Mello, Seva Kashin, Shila Mekhoubad, Dusko Ilic, Maura Charlton, Genevieve Saphier ...Show all 17 authors... , Robert E. Handsaker, Giulio Genovese, Shiran Bar, Nissim Benvenisty, Steven A. McCarroll, Kevin Carl Eggan; Show fewer authors
Human pluripotent stem cells recurrently acquire and expand dominant negative p53 mutations.
Nature 545:229-233(2017)

PubMed=30388422; DOI=10.1016/j.stem.2018.10.009
Tenneille E. Ludwig, Angela Kujak, Antonio Rauti, Steven Andrzejewski, Susan Langbehn, James Mayfield, Jacqueline Fuller, Yoshimi Yashiro, Yashushi Hara, Anita Bhattacharyya;
20 years of human pluripotent stem cell research: it all started with five lines.
Cell Stem Cell 23:644-648(2018)

Cross-references
Cell line collections (Providers) WiCell; wa07
WiCell; wa07-pcbc
Cell line databases/resources hPSCreg; WAe007-A
IGRhCellID; H7%20p91
NIHhESC; NIHhESC-10-0061
SKIP; SKIP001934
Anatomy/cell type resources BTO; BTO_0006061
Biological sample resources BioSample; SAMN03471896
ENCODE; ENCBS017AOD
ENCODE; ENCBS052WSJ
ENCODE; ENCBS058YII
ENCODE; ENCBS068KXI
ENCODE; ENCBS291AAA
ENCODE; ENCBS292AAA
ENCODE; ENCBS293AAA
ENCODE; ENCBS297CQV
ENCODE; ENCBS311AAZ
ENCODE; ENCBS336AYE
ENCODE; ENCBS465FZV
ENCODE; ENCBS559YVY
ENCODE; ENCBS611ORI
ENCODE; ENCBS624XJG
ENCODE; ENCBS721YNV
ENCODE; ENCBS851RDN
ENCODE; ENCBS884CKU
ENCODE; ENCBS920PVU
ENCODE; ENCBS962QAF
Encyclopedic resources Wikidata; Q54993513
Experimental variables resources EFO; EFO_0005904
Gene expression databases GEO; GSM347922
GEO; GSM383877
GEO; GSM384099
GEO; GSM499755
GEO; GSM499756
GEO; GSM500935
GEO; GSM510581
GEO; GSM537623
GEO; GSM537639
GEO; GSM537640
GEO; GSM537641
GEO; GSM537657
GEO; GSM580005
GEO; GSM621347
GEO; GSM621353
GEO; GSM637776
GEO; GSM669896
GEO; GSM669926
GEO; GSM736610
GEO; GSM736638
GEO; GSM856730
GEO; GSM862732
GEO; GSM945183
GEO; GSM945184
GEO; GSM945185
GEO; GSM945186
GEO; GSM981283
GEO; GSM1008596
GEO; GSM1022649
GEO; GSM1022656
GEO; GSM1022660
GEO; GSM1022670
GEO; GSM1084991
GEO; GSM1085014
GEO; GSM1215275
GEO; GSM1215276
GEO; GSM1589801
GEO; GSM1589813
GEO; GSM1589933
GEO; GSM1589945
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number37