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Cellosaurus WA07 (CVCL_9772)

[Text version]
Cell line name WA07
Synonyms WA 07; WA-07; WA7; H7; H7 GE07; H7-hESC; ES-WA7; WAe002-A; WICELLe002-A; WAe007-A; WA07-PCBC; PCBC02hse2014030501; SC14-066
Accession CVCL_9772
Resource Identification Initiative To cite this cell line use: WA07 (RRID:CVCL_9772)
Comments Part of: ENCODE project common cell types; tier 3.
Part of: NHBLI Progenitor Cell Biology Consortium (PCBC) collection.
From: University of Wisconsin; Madison; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0061.
Registration: Swiss research registry; BAG-hES-IMP-0034.
Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18/SCSC11-18.
Omics: CTCF ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me1 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K9ac ChIP-seq epigenome analysis.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Transcriptome analysis by serial analysis of gene expression (SAGE).
Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
Donor information: Embryo is sibling to those giving rise to WA13 (Cellosaurus=CVCL_9774) and WA14 (Cellosaurus=CVCL_9775).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
HLA typing Source: PubMed=16919167
Class I
HLA-AA*02:25
HLA-BB*18,35
HLA-CC*04,12
Class II
HLA-DQDQB1*02,03
HLA-DRDRB1*07,11
DRB3*02
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_S798 (H7.s14)CVCL_S799 (H7.s6)CVCL_S800 (H7.s9)
CVCL_A5NJ (H7-H2B-RFP)CVCL_A5LL (H7-YAP1)CVCL_A5LK (H7v1,17q,i20)
CVCL_A5KR (H7v1q)CVCL_A5KS (H7v17q)CVCL_A5KT (H7v20q)
CVCL_A8WK (WAe007-A-1)CVCL_C4MS (WAe007-A-2)CVCL_D0PI (WAe007-A-3)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=16919167; WiCell=wa07

Markers:
AmelogeninX
CSF1PO12
D5S81811,13
D7S82010,11
D13S31711,12
D16S53912,13
TH016
TPOX8,11
vWA14,15

Run an STR similarity search on this cell line
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/human/H7-hESC_StamAndCrawford_protocol.pdf
https://stemcelldb.nih.gov/cellLineDetails.do?id=3
https://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
Publications

PubMed=9804556; DOI=10.1126/science.282.5391.1145
Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M.
Embryonic stem cell lines derived from human blastocysts.
Science 282:1145-1147(1998)

PubMed=14745950; DOI=10.1002/dvdy.10431
Carpenter M.K., Rosler E.S., Fisk G.J., Brandenberger R., Ares X., Miura T., Lucero M.T., Rao M.S.
Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
Dev. Dyn. 229:243-258(2004)

PubMed=15345125; DOI=10.1089/scd.2004.13.325
Draper J.S., Moore H.D., Ruban L.N., Gokhale P.J., Andrews P.W.
Culture and characterization of human embryonic stem cells.
Stem Cells Dev. 13:325-336(2004)

PubMed=16388305; DOI=10.1038/nbt1177
Ludwig T.E., Levenstein M.E., Jones J.M., Berggren W.T., Mitchen E.R., Frane J.L., Crandall L.J., Daigh C.A., Conard K.R., Piekarczyk M.S., Llanas R.A., Thomson J.A.
Derivation of human embryonic stem cells in defined conditions.
Nat. Biotechnol. 24:185-187(2006)

PubMed=16919167; DOI=10.1186/1741-7007-4-28; PMCID=PMC1601965
Josephson R., Sykes G.R., Liu Y., Ording C.J., Xu W.-N., Zeng X.-M., Shin S., Loring J.F., Maitra A., Rao M.S., Auerbach J.M.
A molecular scheme for improved characterization of human embryonic stem cell lines.
BMC Biol. 4:28.1-28.13(2006)

PubMed=17287758; DOI=10.1038/nbt1285
Baker D.E.C., Harrison N.J., Maltby E., Smith K., Moore H.D., Shaw P.J., Heath P.R., Holden H., Andrews P.W.
Adaptation to culture of human embryonic stem cells and oncogenesis in vivo.
Nat. Biotechnol. 25:207-215(2007)

PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113; PMCID=PMC2394759
Hirst M., Delaney A., Rogers S.A., Schnerch A., Persaud D.R., O'Connor M.D., Zeng T., Moksa M., Fichter K., Mah D., Go A., Morin R.D., Baross A., Zhao Y.-J., Khattra J., Prabhu A.-L., Pandoh P., McDonald H., Asano J.K., Dhalla N., Ma K., Lee S., Ally A., Chahal N., Menzies S., Siddiqui A., Holt R.A., Jones S.J.M., Gerhard D.S., Thomson J.A., Eaves C.J., Marra M.A.
LongSAGE profiling of nine human embryonic stem cell lines.
Genome Biol. 8:R113.1-R113.12(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.-H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.-W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M.J., Pedersen R., Pera M.F., Piekarczyk M.S., Reijo Pera R.A., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T.T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward-van Oostwaard D., Weaver T.A., Young L.A., Zhang W.-D.
International Stem Cell Initiative
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032; PMCID=PMC3063454
Bock C., Kiskinis E., Verstappen G., Gu H.-C., Boulting G.L., Smith Z.D., Ziller M.J., Croft G.F., Amoroso M.W., Oakley D.H., Gnirke A., Eggan K.C., Meissner A.
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=21983960; DOI=10.1038/nmeth.1699; PMCID=PMC3432645
Phanstiel D.H., Brumbaugh J., Wenger C.D., Tian S.-L., Probasco M.D., Bailey D.J., Swaney D.L., Tervo M.A., Bolin J.M., Ruotti V., Stewart R., Thomson J.A., Coon J.J.
Proteomic and phosphoproteomic comparison of human ES and iPS cells.
Nat. Methods 8:821-827(2011)

PubMed=22315618; DOI=10.1155/2012/875059; PMCID=PMC3270482
Pekkanen-Mattila M., Ojala M., Kerkela E., Rajala K., Skottman H., Aalto-Setala K.
The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
Stem Cells Int. 2012:875059.1-875059.10(2012)

PubMed=23117585; DOI=10.1016/j.scr.2012.09.002; PMCID=PMC3590800
Mallon B.S., Chenoweth J.G., Johnson K.R., Hamilton R.S., Tesar P.J., Yavatkar A.S., Tyson L.J., Park K., Chen K.G., Fann Y.C., McKay R.D.G.
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
Stem Cell Res. 10:57-66(2013)

PubMed=23341440; DOI=10.5966/sctm.2012-0047; PMCID=PMC3659749
Toivonen S., Ojala M., Hyysalo A., Ilmarinen T., Rajala K., Pekkanen-Mattila M., Aanismaa R., Lundin K., Palgi J., Weltner J., Trokovic R., Silvennoinen O., Skottman H., Narkilahti S., Aalto-Setala K., Otonkoski T.
Comparative analysis of targeted differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines.
Stem Cells Transl. Med. 2:83-93(2013)

PubMed=27293150; DOI=10.1016/j.stemcr.2016.05.006; PMCID=PMC4944587
Salomonis N., Dexheimer P.J., Omberg L., Schroll R., Bush S., Huo J.S., Schriml L.M., Sui S.H., Keddache M., Mayhew C.N., Shanmukhappa S.K., Wells J.M., Daily K., Hubler S., Wang Y.-L., Zambidis E.T., Margolin A.A., Hide W.A., Hatzopoulos A.K., Malik P., Cancelas J.A., Aronow B.J., Lutzko C.
Integrated genomic analysis of diverse induced pluripotent stem cells from the Progenitor Cell Biology Consortium.
Stem Cell Reports 7:110-125(2016)

PubMed=27688775; DOI=10.1155/2016/3826249; PMCID=PMC5023833
Nagaria P.K., Robert C., Park T.S., Huo J.S., Zambidis E.T., Rassool F.V.
High-fidelity reprogrammed human IPSCs have a high efficacy of DNA repair and resemble hESCs in their MYC transcriptional signature.
Stem Cells Int. 2016:3826249.1-3826249.14(2016)

PubMed=28445466; DOI=10.1038/nature22312; PMCID=PMC5427175
Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C., Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G., Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A., Eggan K.C.
Human pluripotent stem cells recurrently acquire and expand dominant negative p53 mutations.
Nature 545:229-233(2017)

PubMed=30388422; DOI=10.1016/j.stem.2018.10.009
Ludwig T.E., Kujak A., Rauti A., Andrzejewski S., Langbehn S., Mayfield J., Fuller J., Yashiro Y., Hara Y., Bhattacharyya A.
20 years of human pluripotent stem cell research: it all started with five lines.
Cell Stem Cell 23:644-648(2018)

Cross-references
Cell line collections (Providers) WiCell; wa07
WiCell; wa07-pcbc
Cell line databases/resources hPSCreg; WAe007-A
IGRhCellID; H7%20p91
NIHhESC; NIHhESC-10-0061
SKIP; SKIP001934
Anatomy/cell type resources BTO; BTO:0006061
Biological sample resources BioSample; SAMN03471896
ENCODE; ENCBS017AOD
ENCODE; ENCBS052WSJ
ENCODE; ENCBS058YII
ENCODE; ENCBS068KXI
ENCODE; ENCBS291AAA
ENCODE; ENCBS292AAA
ENCODE; ENCBS293AAA
ENCODE; ENCBS297CQV
ENCODE; ENCBS311AAZ
ENCODE; ENCBS336AYE
ENCODE; ENCBS465FZV
ENCODE; ENCBS559YVY
ENCODE; ENCBS611ORI
ENCODE; ENCBS624XJG
ENCODE; ENCBS721YNV
ENCODE; ENCBS851RDN
ENCODE; ENCBS884CKU
ENCODE; ENCBS920PVU
ENCODE; ENCBS962QAF
Encyclopedic resources Wikidata; Q54993513
Experimental variables resources EFO; EFO_0005904
Gene expression databases GEO; GSM347922
GEO; GSM383877
GEO; GSM384099
GEO; GSM499755
GEO; GSM499756
GEO; GSM500935
GEO; GSM510581
GEO; GSM537623
GEO; GSM537639
GEO; GSM537640
GEO; GSM537641
GEO; GSM537657
GEO; GSM580005
GEO; GSM621347
GEO; GSM621353
GEO; GSM637776
GEO; GSM669896
GEO; GSM669926
GEO; GSM736610
GEO; GSM736638
GEO; GSM856730
GEO; GSM862732
GEO; GSM945183
GEO; GSM945184
GEO; GSM945185
GEO; GSM945186
GEO; GSM981283
GEO; GSM1008596
GEO; GSM1022649
GEO; GSM1022656
GEO; GSM1022660
GEO; GSM1022670
GEO; GSM1084991
GEO; GSM1085014
GEO; GSM1215275
GEO; GSM1215276
GEO; GSM1589801
GEO; GSM1589813
GEO; GSM1589933
GEO; GSM1589945
Entry history
Entry creation06-Jun-2012
Last entry update10-Sep-2024
Version number35