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Cellosaurus WA14 (CVCL_9775)

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Cell line name WA14
Synonyms WA 14; H14; GE14; WAe005-A; WICELLe005-A; WAe014-A
Accession CVCL_9775
Resource Identification Initiative To cite this cell line use: WA14 (RRID:CVCL_9775)
Comments Group: Clinical grade pluripotent cell line.
From: University of Wisconsin; Madison; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0064.
Registration: Swiss research registry; BAG-hES-IMP-0033.
Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18/SCSC11-18.
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics.
Omics: Proteomics; PTM; Phosphorylation.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; Serial analysis of gene expression (SAGE).
Omics: Variations; SNP array analysis.
Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
Donor information: Embryo is sibling to those giving rise to WA07 (Cellosaurus=CVCL_9772) and WA13 (Cellosaurus=CVCL_9774).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A5NK (H14-H2B-RFP)CVCL_A5LM (H14-YAP1)CVCL_A5KU (H14.BJ1)
CVCL_C3IU (H14.s3)CVCL_A5KV (H14.s9)CVCL_U174 (NSC-H14)
CVCL_C3LV (WA14 GLA KO clone 01-56)CVCL_C3LW (WA14 GLA KO clone 334-04)CVCL_A5QX (WAe014-A-1)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): WiCell=wa14

Markers:
AmelogeninX,Y
CSF1PO11,12
D3S135815,16
D5S81811,13
D7S82010,11
D8S117913,14
D13S31711
D16S53911,13
D18S5112,14
D21S1130,31
FGA21
Penta D12,13
Penta E13,20
TH016,7
TPOX8
vWA15,16

Run an STR similarity search on this cell line
Web pages Info; -; -; https://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
Info; StemCellDB; -; https://stemcelldb.nih.gov/cellLineDetails/Agilent/29
Publications

PubMed=9804556; DOI=10.1126/science.282.5391.1145
James Alexander Thomson, Joseph Itskovitz-Eldor, Sander S. Shapiro, Michelle A. Waknitz, Jennifer J. Swiergiel, Vivienne S. Marshall, Jeffrey M. Jones;
Embryonic stem cell lines derived from human blastocysts.
Science 282:1145-1147(1998)

PubMed=14745950; DOI=10.1002/dvdy.10431
Melissa K. Carpenter, Elen S. Rosler, Gregory J. Fisk, Ralph Brandenberger, Ximena Ares, Takumi Miura, Mary Theresa Lucero, Mahendra Surendra Rao;
Properties of four human embryonic stem cell lines maintained in a feeder-free culture system.
Dev. Dyn. 229:243-258(2004)

PubMed=16388305; DOI=10.1038/nbt1177
Tenneille E. Ludwig, Mark E. Levenstein, Jeffrey M. Jones, W. Travis Berggren, Erika R. Mitchen, Jennifer L. Frane, Leann J. Crandall, Christine A. Daigh, Kevin R. Conard, Marian S. Piekarczyk, Rachel A. Llanas, James Alexander Thomson;
Derivation of human embryonic stem cells in defined conditions.
Nat. Biotechnol. 24:185-187(2006)

PubMed=17287758; DOI=10.1038/nbt1285
Duncan E.C. Baker, Neil J. Harrison, Edna Maltby, Kath Smith, Harry D. Moore, Pamela Jean Shaw, Paul R. Heath, Hazel Holden, Peter W. Andrews;
Adaptation to culture of human embryonic stem cells and oncogenesis in vivo.
Nat. Biotechnol. 25:207-215(2007)

PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113; PMCID=PMC2394759
Martin Hirst, Allen Delaney, Sean A. Rogers, Angelique Schnerch, Deryck R. Persaud, Michael D. O'Connor, Thomas Zeng, Michelle Moksa, Keith Fichter, Diana Mah, Anne Go ...Show all 32 authors... , Ryan D. Morin, Agnes Baross, Yong-Jun Zhao, Jaswinder Khattra, Anna-Liisa Prabhu, Pawan Pandoh, Helen McDonald, Jennifer K. Asano, Noreen Dhalla, Kevin Ma, Stephanie Lee, Adrian Ally, Neil Chahal, Stephanie Menzies, Asim Siddiqui, Robert A. Holt, Steven J.M. Jones, Daniela S. Gerhard, James Alexander Thomson, Constance Jean Eaves, Marco Antonio Marra; Show fewer authors
LongSAGE profiling of nine human embryonic stem cell lines.
Genome Biol. 8:R113.1-R113.12(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W. Andrews, Gemma Beighton, Paul A. Bello, Nissim Benvenisty, Lorraine S. Berry, Simon Bevan, Barak Blum ...Show all 85 authors... , Justin Brooking, Kevin G. Chen, Andre B.-H. Choo, Gary A. Churchill, Marie Corbel, Ivan Damjanov, Jonathan S. Draper, Petr Dvorak, Katarina Emanuelsson, Roland A. Fleck, Angela Ford, Karin Gertow, Marina Gertsenstein, Paul J. Gokhale, Rebecca S. Hamilton, Ales Hampl, Lyn E. Healy, Outi Hovatta, Johan Hyllner, Marta P. Imreh, Joseph Itskovitz-Eldor, Jamie Jackson, Jacqueline L. Johnson, Mark Jones, Kehkooi Kee, Benjamin L. King, Barbara B. Knowles, Majlinda Lako, Franck Lebrin, Barbara S. Mallon, Daisy Manning, Yoav Mayshar, Ronald Davies Graham McKay, Anna E. Michalska, Milla Mikkola, Maria Mileikovsky, Stephen Lewis Minger, Harry D. Moore, Christine L. Mummery, Andras Nagy, Norio Nakatsuji, Carmel M. O'Brien, Steve Kah-Weng Oh, Cia Olsson, Timo Otonkoski, Kye-Yoon Park, Robert Passier, Hema Patel, Minal J. Patel, Roger Arnold Pedersen, Martin F. Pera, Marian S. Piekarczyk, Renee A. Reijo Pera, Benjamin E. Reubinoff, Allan J. Robins, Janet Rossant, Peter Rugg-Gunn, Thomas C. Schulz, Henrik Semb, Eric S. Sherrer, Henrike Siemen, Glyn N. Stacey, Miodrag Stojkovic, Hirofumi Suemori, Jin Szatkiewicz, Tikva Tako Turetsky, Timo Tuuri, Stieneke van den Brink, Kristina Vintersten, Sanna Vuoristo, Dorien Ward-van Oostwaard, Thomas A. Weaver, Lesley A. Young, Wei-Dong Zhang; Show fewer authors
International Stem Cell Initiative
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=21983960; DOI=10.1038/nmeth.1699; PMCID=PMC3432645
Douglas H. Phanstiel, Justin Brumbaugh, Craig D. Wenger, Shu-Lan Tian, Mitchell D. Probasco, Derek J. Bailey, Danielle L. Swaney, Mark A. Tervo, Jennifer M. Bolin, Victor Ruotti, Ron Stewart ...Show all 13 authors... , James Alexander Thomson, Joshua J. Coon; Show fewer authors
Proteomic and phosphoproteomic comparison of human ES and iPS cells.
Nat. Methods 8:821-827(2011)

PubMed=23117585; DOI=10.1016/j.scr.2012.09.002; PMCID=PMC3590800
Barbara S. Mallon, Joshua G. Chenoweth, Kory R. Johnson, Rebecca S. Hamilton, Paul J. Tesar, Amarendra S. Yavatkar, Leonard J. Tyson, Kyeyoon Park, Kevin G. Chen, Yang-Cheng Fann, Ronald Davies Graham McKay;
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
Stem Cell Res. 10:57-66(2013)

PubMed=28445466; DOI=10.1038/nature22312; PMCID=PMC5427175
Florian T. Merkle, Sulagna Ghosh, Nolan Kamitaki, Jana Mitchell, Yishai Avior, Curtis Mello, Seva Kashin, Shila Mekhoubad, Dusko Ilic, Maura Charlton, Genevieve Saphier ...Show all 17 authors... , Robert E. Handsaker, Giulio Genovese, Shiran Bar, Nissim Benvenisty, Steven A. McCarroll, Kevin Carl Eggan; Show fewer authors
Human pluripotent stem cells recurrently acquire and expand dominant negative p53 mutations.
Nature 545:229-233(2017)

PubMed=30388422; DOI=10.1016/j.stem.2018.10.009
Tenneille E. Ludwig, Angela Kujak, Antonio Rauti, Steven Andrzejewski, Susan Langbehn, James Mayfield, Jacqueline Fuller, Yoshimi Yashiro, Yashushi Hara, Anita Bhattacharyya;
20 years of human pluripotent stem cell research: it all started with five lines.
Cell Stem Cell 23:644-648(2018)

Cross-references
Cell line collections (Providers) WiCell; wa14
WiCell; wa14-cgmp-material
WiCell; wa14-matched-research
Cell line databases/resources hPSCreg; WAe014-A
NIHhESC; NIHhESC-10-0064
SKIP; SKIP001937
Biological sample resources BioSample; SAMN03471899
Encyclopedic resources Wikidata; Q54993517
Gene expression databases GEO; GSM151738
GEO; GSM151740
GEO; GSM347925
GEO; GSM383876
GEO; GSM384098
GEO; GSM856733
GEO; GSM862739
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number30