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Cellosaurus WA13 (CVCL_9774)

[Text version]
Cell line name WA13
Synonyms WA 13; H13; H13C; GE13; WAe004-A; WICELLe004-A; WAe013-A
Accession CVCL_9774
Resource Identification Initiative To cite this cell line use: WA13 (RRID:CVCL_9774)
Comments From: University of Wisconsin; Madison; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0063.
Registration: UK Stem Cell Bank (UKSCB); Steering comm. appl. SCSC06-18.
Omics: Deep exome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by serial analysis of gene expression (SAGE).
Anecdotal: WA01, WA07, WA09, WA13 and WA14 were the first human embryonic stem cells to be established.
Donor information: Embryo is sibling to those giving rise to WA07 (Cellosaurus=CVCL_9772) and WA14 (Cellosaurus=CVCL_9775).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C0CE (WAe013-A-1)CVCL_C0CF (WAe013-A-2)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): WiCell

Markers:
AmelogeninX,Y
CSF1PO12
D5S81811,13
D7S82010,11
D13S31711,12
D16S5399,11
TH016
TPOX8,11
vWA14,15

Run an STR similarity search on this cell line
Web pages https://stemcelldb.nih.gov/cellLineDetails.do?id=4
https://news.wisc.edu/nih-reapproves-wicells-h9-and-three-other-wisconsin-stem-cell-lines-for-federally-funded-research/
Publications

PubMed=9804556; DOI=10.1126/science.282.5391.1145
Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M.
Embryonic stem cell lines derived from human blastocysts.
Science 282:1145-1147(1998)

PubMed=17570852; DOI=10.1186/gb-2007-8-6-r113
Hirst M., Delaney A., Rogers S.A., Schnerch A., Persaud D.R., O'Connor M.D., Zeng T., Moksa M., Fichter K., Mah D., Go A., Morin R.D., Baross A., Zhao Y.-J., Khattra J., Prabhu A.-L., Pandoh P., McDonald H., Asano J.K., Dhalla N., Ma K., Lee S., Ally A., Chahal N., Menzies S., Siddiqui A., Holt R.A., Jones S.J.M., Gerhard D.S., Thomson J.A., Eaves C.J., Marra M.A.
LongSAGE profiling of nine human embryonic stem cell lines.
Genome Biol. 8:R113.1-R113.12(2007)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.-H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.-W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M.J., Pedersen R., Pera M.F., Piekarczyk M.S., Reijo Pera R.A., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T.T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward-van Oostwaard D., Weaver T.A., Young L.A., Zhang W.-D.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=20736931; DOI=10.1038/mt.2010.179
Ramos-Mejia V., Melen G.J., Sanchez L., Gutierrez-Aranda I., Ligero G., Cortes J.L., Real P.J., Bueno C., Menendez P.
Nodal/activin signaling predicts human pluripotent stem cell lines prone to differentiate toward the hematopoietic lineage.
Mol. Ther. 18:2173-2181(2010)

PubMed=21936705; DOI=10.1089/scd.2011.0400
Ramos-Mejia V., Fernandez A.F., Ayllon V., Real P.J., Bueno C., Anderson P., Martin F., Fraga M.F., Menendez P.
Maintenance of human embryonic stem cells in mesenchymal stem cell-conditioned media augments hematopoietic specification.
Stem Cells Dev. 21:1549-1558(2012)

PubMed=23117585; DOI=10.1016/j.scr.2012.09.002
Mallon B.S., Chenoweth J.G., Johnson K.R., Hamilton R.S., Tesar P.J., Yavatkar A.S., Tyson L.J., Park K., Chen K.G., Fann Y.C., McKay R.D.G.
StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
Stem Cell Res. 10:57-66(2013)

PubMed=28445466; DOI=10.1038/nature22312
Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C., Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G., Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A., Eggan K.C.
Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.
Nature 545:229-233(2017)

PubMed=30388422; DOI=10.1016/j.stem.2018.10.009
Ludwig T.E., Kujak A., Rauti A., Andrzejewski S., Langbehn S., Mayfield J., Fuller J., Yashiro Y., Hara Y., Bhattacharyya A.
20 years of human pluripotent stem cell research: it all started with five lines.
Cell Stem Cell 23:644-648(2018)

Cross-references
Cell line collections (Providers) WiCell; wa13
Cell line databases/resources hPSCreg; WAe013-A
ISCR; 92
NIHhESC; NIHhESC-10-0063
SKIP; SKIP001936
Biological sample resources BioSample; SAMN03471898
Encyclopedic resources Wikidata; Q54993516
Gene expression databases GEO; GSM288396
GEO; GSM347924
GEO; GSM383875
GEO; GSM384097
GEO; GSM755489
GEO; GSM755492
GEO; GSM856732
GEO; GSM862738
Entry history
Entry creation06-Jun-2012
Last entry update29-Jun-2023
Version number26