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Cellosaurus KMST-6 (CVCL_2998)

[Text version]

Cell line name KMST-6
Synonyms KMST6; Kawasaki Medical School Transformed-6
Accession CVCL_2998
Resource Identification Initiative To cite this cell line use: KMST-6 (RRID:CVCL_2998)
Comments Population: Japanese.
Characteristics: Cell line positive for alternative lengthening of telomeres (ALT+) (CelloPub=CLPUB00712; PubMed=19935656).
Doubling time: 38 +- 4.6 hours (in 10% FBS), 54 +- 2.9 hours (in 1% FBS) (PubMed=1537630); ~26 hours (lot 12242014) (JCRB).
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from sampling site: Whole embryo. Cell type=Fibroblast.
Sequence variations
  • Mutation; HGNC; 11998; TP53; Simple; p.His179Pro (c.536A>C); ClinVar=VCV000376611; Zygosity=Heterozygous (PubMed=8790940).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2997 (KMS-6)
CVCL_WH81 (KMST-6/p53 )CVCL_M810 (KMST-6/RAS )CVCL_2999 (KMST-6/T )
Sex of cell Female
Age at sampling 9FW
Category Transformed cell line
STR profile Source(s): JCRB; RCB; TKG

12 (RCB; TKG)

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/k/cell-lines-detail-172.html

PubMed=3972471; DOI=10.1002/ijc.2910350221
Namba M., Nishitani K., Hyodoh F., Fukushima F., Kimoto T.
Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with 60Co gamma rays.
Int. J. Cancer 35:275-280(1985)

PubMed=2423124; DOI=10.1016/0304-419X(86)90001-6
Chang S.E.
In vitro transformation of human epithelial cells.
Biochim. Biophys. Acta 823:161-194(1986)

Namba M., Nishitani K., Fukushima F., Kimoto T.
Multistep carcinogenesis of normal human fibroblasts. Human fibroblasts immortalized by repeated treatment with Co-60 gamma rays were transformed into tumorigenic cells with Ha-ras oncogenes.
Anticancer Res. 8:947-958(1988)

PubMed=1537630; DOI=10.1002/ijc.2910500426
Mihara K., Bai L., Kano Y., Miyazaki M., Namba M.
Malignant transformation of human fibroblasts previously immortalized with 60Co gamma rays.
Int. J. Cancer 50:639-643(1992)

Namba M., Bai L., Kano Y., Sugihara S., Miyazaki M.
Multistep malignant transformation of human cells: immortalization of human fibroblasts by treatment with 60Co gamma rays and 4-nitroquinoline 1-oxide, and their malignant transformation with the ras oncogene.
Tissue Cult. Res. Commun. 12:313-318(1993)

PubMed=8105370; DOI=10.1128/mcb.13.10.6036-6043.1993
Ogata T., Ayusawa D., Namba M., Takahashi E., Oshimura M., Oishi M.
Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.
Mol. Cell. Biol. 13:6036-6043(1993)

Namba M., Iijima M., Kondo T., Jahan I., Mihara K.
Immortalization of normal human cells is a multistep process and a rate limiting step of neoplastic transformation of the cells.
Hum. Cell 6:253-259(1993)

PubMed=8557247; DOI=10.1007/BF00218823
Sugihara S., Mihara K., Marunouchi T., Inoue H., Namba M.
Telomere elongation observed in immortalized human fibroblasts by treatment with 60Co gamma rays or 4-nitroquinoline 1-oxide.
Hum. Genet. 97:1-6(1996)

PubMed=8790940; DOI=10.1247/csf.21.111
Mihara K., Iijima M., Kondo T., Namba M.
Selective expression of mutated p53 in human cells immortalized with either 4-nitroquinoline 1-oxide or 60Co gamma rays.
Cell Struct. Funct. 21:111-116(1996)

Mihara K., Miyazaki M., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ohashi R., Namba M.
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9359923; DOI=10.18926/AMO/30789
Mihara K., Miyazaki M., Kondo T., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ishioka C., Namba M.
Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
Acta Med. Okayama 51:261-265(1997)

Henson J.D.
The role of alternative lengthening of telomeres in human cancer.
Thesis PhD (2006), University of Sydney, Australia

PubMed=19935656; DOI=10.1038/nbt.1587
Henson J.D., Cao Y., Huschtscha L.I., Chang A.C., Au A.Y.M., Pickett H.A., Reddel R.R.
DNA C-circles are specific and quantifiable markers of alternative-lengthening-of-telomeres activity.
Nat. Biotechnol. 27:1181-1185(2009)

Cell line collections JCRB; JCRB0433
RCB; RCB1955
TKG; TKG 0482
Cell line databases/resources CLO; CLO_0050468
Anatomy/cell type resources BTO; BTO:0002039
Biological sample resources BioSample; SAMN03471629
BioSample; SAMN03472044
Encyclopedic resources Wikidata; Q54900187
Polymorphism and mutation databases IARC_TP53; 5358
Entry history
Entry creation04-Apr-2012
Last entry update22-Sep-2022
Version number23