ID   KMST-6
AC   CVCL_2998
SY   KMST6; Kawasaki Medical School Transformed-6
DR   BTO; BTO_0002039
DR   CLO; CLO_0050468
DR   BioSample; SAMN03471629
DR   BioSample; SAMN03472044
DR   IARC_TP53; 5358
DR   JCRB; JCRB0433
DR   RCB; RCB1955
DR   TKG; TKG 0482
DR   Wikidata; Q54900187
RX   CelloPub=CLPUB00712;
RX   DOI=10.11418/jtca1981.12.4_313;
RX   DOI=10.11418/jtca1981.16.3_173;
RX   PubMed=1537630;
RX   PubMed=2423124;
RX   PubMed=3140712;
RX   PubMed=3972471;
RX   PubMed=8105370;
RX   PubMed=8148305;
RX   PubMed=8557247;
RX   PubMed=8790940;
RX   PubMed=9290701;
RX   PubMed=9359923;
RX   PubMed=19935656;
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/k/cell-lines-detail-172.html
CC   Population: Japanese.
CC   Characteristics: Cell line positive for alternative lengthening of telomeres (ALT+) (CelloPub=CLPUB00712; PubMed=19935656).
CC   Doubling time: 38 +- 4.6 hours (Note=In 10% FBS), 54 +- 2.9 hours (Note=In 1% FBS) (PubMed=1537630); ~26 hours (Note=Lot 12242014) (JCRB=JCRB0433).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Pro (c.536A>C); ClinVar=VCV000376611; Zygosity=Heterozygous (PubMed=8790940).
CC   Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
CC   Derived from site: In situ; Whole fetus; UBERON=UBERON_0000323.
CC   Cell type: Fibroblast; CL=CL_0000057.
ST   Source(s): JCRB=JCRB0433; RCB=RCB1955; TKG=TKG 0482
ST   Amelogenin: X
ST   CSF1PO: 9,12 (JCRB=JCRB0433)
ST   CSF1PO: 12 (RCB=RCB1955; TKG=TKG 0482)
ST   D13S317: 10,11
ST   D16S539: 11
ST   D5S818: 10,12
ST   D7S820: 9
ST   TH01: 9
ST   TPOX: 11,12
ST   vWA: 14,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_2997 ! KMS-6
SX   Female
AG   9FW
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 29
CH   CVCL_WH81 ! KMST-6/p53
CH   CVCL_M810 ! KMST-6/RAS
CH   CVCL_2999 ! KMST-6/T
CH   CVCL_A447 ! KMST-6/TNF
//
RX   PubMed=19935656; DOI=10.1038/nbt.1587;
RA   Henson, Jeremy David
RA   Cao, Ying
RA   Huschtscha, Lily I.
RA   Chang, Andy C.-M.
RA   Au, Amy Y.M.
RA   Pickett, Hilda A.
RA   Reddel, Roger Robert
RT   "DNA C-circles are specific and quantifiable markers of
RT   alternative-lengthening-of-telomeres activity.";
RL   Nat. Biotechnol. 27:1181-1185(2009).
//
RX   PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D;
RA   Jia, Li-Qun
RA   Osada, Motonobu
RA   Ishioka, Chikashi
RA   Gamo, Makio
RA   Ikawa, Shuntaro
RA   Suzuki, Takao
RA   Shimodaira, Hideki
RA   Niitani, Tomohito
RA   Kudo, Toshio
RA   Akiyama, Mitoshi
RA   Kimura, Narimiti
RA   Matsuo, Mitsuyoshi
RA   Mizusawa, Hiroshi
RA   Tanaka, Noriho
RA   Koyama, Hideki
RA   Namba, Masayoshi
RA   Kanamaru, Ryunosuke
RA   Kuroki, Toshio
RT   "Screening the p53 status of human cell lines using a yeast functional
RT   assay.";
RL   Mol. Carcinog. 19:243-253(1997).
//
RX   PubMed=1537630; DOI=10.1002/ijc.2910500426;
RA   Mihara, Koichiro
RA   Bai, Li-Yan
RA   Kano, Yoshio
RA   Miyazaki, Masahiro
RA   Namba, Masayoshi
RT   "Malignant transformation of human fibroblasts previously immortalized
RT   with 60Co gamma rays.";
RL   Int. J. Cancer 50:639-643(1992).
//
RX   PubMed=8105370; DOI=10.1128/mcb.13.10.6036-6043.1993; PMCID=PMC364663;
RA   Ogata, Toshihiko
RA   Ayusawa, Dai
RA   Namba, Masayoshi
RA   Takahashi, Eiichi
RA   Oshimura, Mitsuo
RA   Oishi, Michio
RT   "Chromosome 7 suppresses indefinite division of nontumorigenic
RT   immortalized human fibroblast cell lines KMST-6 and SUSM-1.";
RL   Mol. Cell. Biol. 13:6036-6043(1993).
//
RX   DOI=10.11418/jtca1981.12.4_313;
RA   Namba, Masayoshi
RA   Bai, Li-Yan
RA   Kano, Yoshio
RA   Sugihara, Shigetaka
RA   Miyazaki, Masahiro
RT   "Multistep malignant transformation of human cells: immortalization of
RT   human fibroblasts by treatment with 60Co gamma rays and 4-nitroquinoline
RT   1-oxide, and their malignant transformation with the ras oncogene.";
RL   Tissue Cult. Res. Commun. 12:313-318(1993).
//
RX   PubMed=9359923; DOI=10.18926/AMO/30789;
RA   Mihara, Koichiro
RA   Miyazaki, Masahiro
RA   Kondo, Tadashi
RA   Fushimi, Kazuo
RA   Tsuji, Toshiya
RA   Inoue, Yusuke
RA   Fukaya, Ken-ichi
RA   Ishioka, Chikashi
RA   Namba, Masayoshi
RT   "Yeast functional assay of the p53 gene status in human cell lines
RT   maintained in our laboratory.";
RL   Acta Med. Okayama 51:261-265(1997).
//
RX   PubMed=8148305;
RA   Namba, Masayoshi
RA   Iijima, Mikio
RA   Kondo, Tadashi
RA   Jahan, Israt
RA   Mihara, Koichiro
RT   "Immortalization of normal human cells is a multistep process and a
RT   rate limiting step of neoplastic transformation of the cells.";
RL   Hum. Cell 6:253-259(1993).
//
RX   PubMed=2423124; DOI=10.1016/0304-419X(86)90001-6;
RA   Chang, Sidney E.
RT   "In vitro transformation of human epithelial cells.";
RL   Biochim. Biophys. Acta 823:161-194(1986).
//
RX   DOI=10.11418/jtca1981.16.3_173;
RA   Mihara, Koichiro
RA   Miyazaki, Masahiro
RA   Fushimi, Kazuo
RA   Tsuji, Toshiya
RA   Inoue, Yusuke
RA   Fukaya, Ken-ichi
RA   Ohashi, Ryuichiro
RA   Namba, Masayoshi
RT   "The p53 gene status and other cellular characteristics of human cell
RT   lines maintained in our laboratory.";
RL   Tissue Cult. Res. Commun. 16:173-178(1997).
//
RX   CelloPub=CLPUB00712;
RA   Henson, Jeremy David
RT   "The role of alternative lengthening of telomeres in human cancer.";
RL   Thesis PhD (2006); University of Sydney; Sydney; Australia.
//
RX   PubMed=3140712;
RA   Namba, Masayoshi
RA   Nishitani, Koji
RA   Fukushima, Fujiko
RA   Kimoto, Tetsuo
RT   "Multistep carcinogenesis of normal human fibroblasts. Human
RT   fibroblasts immortalized by repeated treatment with Co-60 gamma rays
RT   were transformed into tumorigenic cells with Ha-ras oncogenes.";
RL   Anticancer Res. 8:947-958(1988).
//
RX   PubMed=8790940; DOI=10.1247/csf.21.111;
RA   Mihara, Koichiro
RA   Iijima, Mikio
RA   Kondo, Tadashi
RA   Namba, Masayoshi
RT   "Selective expression of mutated p53 in human cells immortalized with
RT   either 4-nitroquinoline 1-oxide or 60Co gamma rays.";
RL   Cell Struct. Funct. 21:111-116(1996).
//
RX   PubMed=8557247; DOI=10.1007/BF00218823;
RA   Sugihara, Shinsuke
RA   Mihara, Koichiro
RA   Marunouchi, Tohru
RA   Inoue, Hajime
RA   Namba, Masayoshi
RT   "Telomere elongation observed in immortalized human fibroblasts by
RT   treatment with 60Co gamma rays or 4-nitroquinoline 1-oxide.";
RL   Hum. Genet. 97:1-6(1996).
//
RX   PubMed=3972471; DOI=10.1002/ijc.2910350221;
RA   Namba, Masayoshi
RA   Nishitani, Koji
RA   Hyodoh, Fuminori
RA   Fukushima, Fujiko
RA   Kimoto, Tetsuo
RT   "Neoplastic transformation of human diploid fibroblasts (KMST-6) by
RT   treatment with 60Co gamma rays.";
RL   Int. J. Cancer 35:275-280(1985).
//