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Cellosaurus LIM1215 (CVCL_2574)

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Cell line name LIM1215
Synonyms Lim1215; LIM 1215; LIM-1215
Accession CVCL_2574
Resource Identification Initiative To cite this cell line use: LIM1215 (RRID:CVCL_2574)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
From: Ludwig Institute for Cancer Research, Melbourne Branch; Melbourne; Australia.
Doubling time: 32 hours, at 8th passage (PubMed=3857372); 28 hours (PubMed=142798).
Microsatellite instability: Instable (MSI) (PubMed=19378335; PubMed=25926053).
Omics: Deep exome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Deep RNAseq analysis.
Omics: N-glycan profiling.
Omics: Transcriptome analysis.
Sequence variations
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Pro423Gln (c.1268C>A); Zygosity=Heterozygous (PubMed=12615714).
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (PubMed=12615714).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Thr41Ala (c.121A>G); ClinVar=VCV000017580; Zygosity=Homozygous (PubMed=24755471; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (PubMed=12615714).
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North3.18
East Asian, South0
South Asian6.91
European, North26.95
European, South57.24
Disease Colon adenocarcinoma (NCIt: C4349)
Lynch syndrome (NCIt: C8494)
Lynch syndrome (ORDO: Orphanet_144)
Derived from metastatic site: Omentum.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_LC77 (LIM1215 KRAS (G12A/+) )CVCL_LC81 (LIM1215 KRAS (G12C/+) )CVCL_LC80 (LIM1215 KRAS (G12D/+) )
CVCL_LC76 (LIM1215 KRAS (G12R/+) )CVCL_LC78 (LIM1215 KRAS (G12S/+) )CVCL_LC79 (LIM1215 KRAS (G12V/+) )
CVCL_LC82 (LIM1215 KRAS (G13D/+) )CVCL_A4IG (LIM1215 PIK3CA (H1047R/+) )CVCL_A4IH (LIM1215 PIK3CA (E545K/+) )
CVCL_A2CA (LIM1215 R1 Cetux )CVCL_A2CB (LIM1215 R2 Cetux )CVCL_A2CC (LIM1215 R3 Cetux )
CVCL_A2CD (LIM1215 R4 Cetux )CVCL_A2CE (LIM1215 R5 Cetux )
Sex of cell Male
Age at sampling 34Y
Category Cancer cell line
STR profile Source(s): CBA; PubMed=11416159; PubMed=25926053

AmelogeninX (PubMed=11416159)
X,Y (CBA; PubMed=25926053)
D8S117911,13 (PubMed=11416159)
13 (CBA)
D16S5398,9,11,12 (PubMed=25926053)
8,12 (CBA)
vWA15,16 (PubMed=25926053)
16 (CBA; PubMed=11416159)

Run an STR similarity search on this cell line

PubMed=3857372; DOI=10.1093/jnci/74.4.759
Whitehead R.H., Macrae F.A., St John D.J.B., Ma J.
A colon cancer cell line (LIM1215) derived from a patient with inherited nonpolyposis colorectal cancer.
J. Natl. Cancer Inst. 74:759-765(1985)

PubMed=1427983; DOI=10.1038/icb.1992.30
Whitehead R.H., Zhang H.H., Hayward I.P.
Retention of tissue-specific phenotype in a panel of colon carcinoma cell lines: relationship to clinical correlates.
Immunol. Cell Biol. 70:227-236(1992)

PubMed=9328464; DOI=10.1093/hmg/6.12.2011
Kilian A., Bowtell D.D.L., Abud H.E., Hime G.R., Venter D.J., Keese P.K., Duncan E.L., Reddel R.R., Jefferson R.A.
Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types.
Hum. Mol. Genet. 6:2011-2019(1997)

Sparks A.B., Morin P.J., Vogelstein B., Kinzler K.W.
Mutational analysis of the APC/beta-catenin/Tcf pathway in colorectal cancer.
Cancer Res. 58:1130-1134(1998)

Hempen P.M., Zhang L., Bansal R.K., Iacobuzio-Donahue C.A., Murphy K.M., Maitra A., Vogelstein B., Whitehead R.H., Markowitz S.D., Willson J.K.V., Yeo C.J., Hruban R.H., Kern S.E.
Evidence of selection for clones having genetic inactivation of the activin A type II receptor (ACVR2) gene in gastrointestinal cancers.
Cancer Res. 63:994-999(2003)

PubMed=19378335; DOI=10.1002/ijc.24289
Zhang H.H., Walker F., Kiflemariam S., Whitehead R.H., Williams D., Phillips W.A., Mikeska T., Dobrovic A., Burgess A.W.
Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B-Raf.
Int. J. Cancer 125:297-307(2009)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192
Janakiraman M., Vakiani E., Zeng Z.-S., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=22722830; DOI=10.1038/nature11156
Misale S., Yaeger R., Hobor S., Scala E., Janakiraman M., Liska D., Valtorta E., Schiavo R., Buscarino M., Siravegna G., Bencardino K., Cercek A., Chen C.-T., Veronese S., Zanon C., Sartore-Bianchi A., Gambacorta M., Gallicchio M., Vakiani E., Boscaro V., Medico E., Weiser M., Siena S., Di Nicolantonio F., Solit D., Bardelli A.
Emergence of KRAS mutations and acquired resistance to anti-EGFR therapy in colorectal cancer.
Nature 486:532-536(2012)

PubMed=23458625; DOI=10.1021/pr3010869
Fanayan S., Smith J.T., Lee L.Y., Yan F., Snyder M., Hancock W.S., Nice E.
Proteogenomic analysis of human colon carcinoma cell lines LIM1215, LIM1899, and LIM2405.
J. Proteome Res. 12:1732-1742(2013)

PubMed=24295106; DOI=10.1021/pr400861m
Sethi M.K., Thaysen-Andersen M., Smith J.T., Baker M.S., Packer N.H., Hancock W.S., Fanayan S.
Comparative N-glycan profiling of colorectal cancer cell lines reveals unique bisecting GlcNAc and alpha-2,3-linked sialic acid determinants are associated with membrane proteins of the more metastatic/aggressive cell lines.
J. Proteome Res. 13:277-288(2014)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25623215; DOI=10.1158/1078-0432.CCR-14-2821
Arena S., Bellosillo B., Siravegna G., Martinez A., Canadas I., Lazzari L., Ferruz N., Russo M., Misale S., Gonzalez I., Iglesias M., Gavilan E., Corti G., Hobor S., Crisafulli G., Salido M., Sanchez J., Dalmases A., Bellmunt J., De Fabritiis G., Rovira A., Di Nicolantonio F., Albanell J., Bardelli A., Montagut C.
Emergence of multiple EGFR extracellular mutations during cetuximab treatment in colorectal cancer.
Clin. Cancer Res. 21:2157-2166(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

PubMed=28854368; DOI=10.1016/j.celrep.2017.08.010
Roumeliotis T.I., Williams S.P., Goncalves E., Alsinet C., Del Castillo Velasco-Herrera M., Aben N., Ghavidel F.Z., Michaut M., Schubert M., Price S., Wright J.C., Yu L., Yang M., Dienstmann R., Guinney J., Beltrao P., Brazma A., Pardo M., Stegle O., Adams D.J., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Choudhary J.S.
Genomic determinants of protein abundance variation in colorectal cancer cells.
Cell Rep. 20:2201-2214(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

Cell line collections CBA; CBA-0161
ECACC; 10092301
Cell line databases/resources Cell_Model_Passport; SIDM00014
ColonAtlas; LIM1215
Cosmic-CLP; 2162949
DepMap; ACH-001546 - Discontinued
Anatomy/cell type resources BTO; BTO:0001916
Biological sample resources BioSample; SAMN07624413
Chemistry resources GDSC; 869795
GDSC; 2162949
PharmacoDB; LIM1215_836_2019
Encyclopedic resources Wikidata; Q54902517
Gene expression databases GEO; GSM741246
GEO; GSM1346878
GEO; GSM1448118
Polymorphism and mutation databases Cosmic; 869795
Cosmic; 1184335
Cosmic; 1238050
Cosmic; 1479632
Cosmic; 1482515
Cosmic; 1486135
Cosmic; 1524335
Cosmic; 1676746
Cosmic; 1805257
Cosmic; 2301991
Cosmic; 2667967
Cosmic; 2787550
Cosmic; 2841366
IARC_TP53; 22464
Proteomic databases PRIDE; PXD000120
PRIDE; PXD005235
Entry history
Entry creation04-Apr-2012
Last entry update16-Dec-2021
Version number32