ID   LIM1215
AC   CVCL_2574
SY   Lim1215; LIM 1215; LIM-1215
DR   BTO; BTO_0001916
DR   EFO; EFO_0022437
DR   BioSample; SAMN07624413
DR   cancercelllines; CVCL_2574
DR   CBA; CBA-0161
DR   Cell_Model_Passport; SIDM00014
DR   ColonAtlas; LIM1215
DR   Cosmic; 869795
DR   Cosmic; 1184335
DR   Cosmic; 1238050
DR   Cosmic; 1479632
DR   Cosmic; 1482515
DR   Cosmic; 1486135
DR   Cosmic; 1524335
DR   Cosmic; 1676746
DR   Cosmic; 1805257
DR   Cosmic; 2301991
DR   Cosmic; 2667967
DR   Cosmic; 2787550
DR   Cosmic; 2841366
DR   Cosmic-CLP; 2162949
DR   DepMap; ACH-001546
DR   ECACC; 10092301
DR   GDSC; 869795
DR   GDSC; 2162949
DR   GEO; GSM741246
DR   GEO; GSM1346878
DR   GEO; GSM1448118
DR   IARC_TP53; 22464
DR   PharmacoDB; LIM1215_836_2019
DR   PRIDE; PXD000120
DR   PRIDE; PXD005235
DR   Wikidata; Q54902517
RX   PubMed=1427983;
RX   PubMed=3857372;
RX   PubMed=9328464;
RX   PubMed=9515795;
RX   PubMed=11416159;
RX   PubMed=12615714;
RX   PubMed=19378335;
RX   PubMed=20570890;
RX   PubMed=22722830;
RX   PubMed=23458625;
RX   PubMed=24295106;
RX   PubMed=24755471;
RX   PubMed=25623215;
RX   PubMed=25926053;
RX   PubMed=25944804;
RX   PubMed=28854368;
RX   PubMed=30894373;
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Part of: COSMIC cell lines project.
CC   From: Ludwig Institute for Cancer Research, Melbourne Branch; Melbourne; Australia.
CC   Population: Caucasian.
CC   Doubling time: 32 hours (Note=At 8th passage) (PubMed=3857372); 28 hours (PubMed=1427983); 29.52 hours (PubMed=25944804).
CC   Microsatellite instability: Instable (MSI) (PubMed=19378335; PubMed=24755471; PubMed=25926053).
CC   Sequence variation: Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Pro423Gln (c.1268C>A); Zygosity=Heterozygous (PubMed=12615714).
CC   Sequence variation: Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (PubMed=12615714).
CC   Sequence variation: Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Thr41Ala (c.121A>G); ClinVar=VCV000017580; Zygosity=Homozygous (PubMed=24755471; Cosmic-CLP=2162949; DepMap=ACH-001546).
CC   Sequence variation: Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (PubMed=12615714).
CC   Omics: Genomics; Whole exome sequencing.
CC   Omics: Glycomics; N-glycan profiling.
CC   Omics: Phenotyping; Drug screening.
CC   Omics: Proteomics; PTM; Phosphorylation; Quantitative.
CC   Omics: Proteomics; Quantitative.
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Transcriptomics; RNAseq.
CC   Genome ancestry: African=5.72%; Native American=0%; East Asian, North=3.18%; East Asian, South=0%; South Asian=6.91%; European, North=26.95%; European, South=57.24% (PubMed=30894373).
CC   Discontinued: DepMap; ACH-001546; true.
CC   Derived from site: Metastatic; Omentum; UBERON=UBERON_0003688.
ST   Source(s): CBA=CBA-0161; PubMed=11416159; PubMed=25926053
ST   Amelogenin: X (PubMed=11416159)
ST   Amelogenin: X,Y (CBA=CBA-0161; PubMed=25926053)
ST   CSF1PO: 10
ST   D13S317: 8,12
ST   D16S539: 8,9,11,12 (PubMed=25926053)
ST   D16S539: 8,12 (CBA=CBA-0161)
ST   D18S51: 12,18
ST   D19S433: 10,15.2
ST   D21S11: 29,31
ST   D2S1338: 16,21
ST   D3S1358: 15,16
ST   D5S818: 9,12
ST   D7S820: 9,10.3
ST   D8S1179: 11,13 (PubMed=11416159)
ST   D8S1179: 13 (CBA=CBA-0161)
ST   FGA: 21,23,24
ST   TH01: 9.3,10
ST   TPOX: 8
ST   vWA: 15,16 (PubMed=25926053)
ST   vWA: 16 (CBA=CBA-0161; PubMed=11416159)
DI   NCIt; C4349; Colon adenocarcinoma
DI   NCIt; C8494; Lynch syndrome
DI   ORDO; Orphanet_144; Lynch syndrome
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   34Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 39
CH   CVCL_E7AP ! LIM1215 IR
CH   CVCL_LC77 ! LIM1215 KRAS (G12A/+)
CH   CVCL_LC81 ! LIM1215 KRAS (G12C/+)
CH   CVCL_LC80 ! LIM1215 KRAS (G12D/+)
CH   CVCL_LC76 ! LIM1215 KRAS (G12R/+)
CH   CVCL_LC78 ! LIM1215 KRAS (G12S/+)
CH   CVCL_LC79 ! LIM1215 KRAS (G12V/+)
CH   CVCL_LC82 ! LIM1215 KRAS (G13D/+)
CH   CVCL_A4IG ! LIM1215 PIK3CA (H1047R/+)
CH   CVCL_A4IH ! LIM1215 PIK3CA (E545K/+)
CH   CVCL_A2CA ! LIM1215 R1 Cetux
CH   CVCL_A2CB ! LIM1215 R2 Cetux
CH   CVCL_A2CC ! LIM1215 R3 Cetux
CH   CVCL_A2CD ! LIM1215 R4 Cetux
CH   CVCL_A2CE ! LIM1215 R5 Cetux
//
RX   PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459;
RA   Masters, John R.W.
RA   Thomson, Jim A.
RA   Daly-Burns, Bernadette
RA   Reid, Yvonne A.
RA   Dirks, Wilhelm Gerhard
RA   Packer, Phil
RA   Toji, Lorraine Hellenga
RA   Ohno, Tadao
RA   Tanabe, Hideyuki
RA   Arlett, Colin Francis
RA   Kelland, Lloyd R.
RA   Harrison, Maureen
RA   Virmani, Arvind K.
RA   Ward, Timothy H.
RA   Ayres, Karen L.
RA   Debenham, Paul G.
RT   "Short tandem repeat profiling provides an international reference
RT   standard for human cell lines.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001).
//
RX   PubMed=3857372; DOI=10.1093/jnci/74.4.759;
RA   Whitehead, Robert H.
RA   Macrae, Finlay A.
RA   St John, D. James B.
RA   Ma, Jeng
RT   "A colon cancer cell line (LIM1215) derived from a patient with
RT   inherited nonpolyposis colorectal cancer.";
RL   J. Natl. Cancer Inst. 74:759-765(1985).
//
RX   PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013;
RA   Mouradov, Dmitri
RA   Sloggett, Clare
RA   Jorissen, Robert N.
RA   Love, Christopher G.
RA   Li, Shan
RA   Burgess, Antony Wilks
RA   Arango, Diego
RA   Strausberg, Robert L.
RA   Buchanan, Daniel
RA   Wormald, Samuel
RA   O'Connor, Liam
RA   Wilding, Jennifer L.
RA   Bicknell, David C.
RA   Tomlinson, Ian Philip Mark
RA   Bodmer, Walter Fred
RA   Mariadason, John M.
RA   Sieber, Oliver M.
RT   "Colorectal cancer cell lines are representative models of the main
RT   molecular subtypes of primary cancer.";
RL   Cancer Res. 74:3238-3247(2014).
//
RX   PubMed=12615714;
RA   Hempen, Paula M.
RA   Zhang, Lin
RA   Bansal, Ravi K.
RA   Iacobuzio-Donahue, Christine A.
RA   Murphy, Kathleen M.
RA   Maitra, Anirban
RA   Vogelstein, Bert
RA   Whitehead, Robert H.
RA   Markowitz, Sanford David
RA   Willson, James K.V.
RA   Yeo, Charles J.
RA   Hruban, Ralph H.
RA   Kern, Scott E.
RT   "Evidence of selection for clones having genetic inactivation of the
RT   activin A type II receptor (ACVR2) gene in gastrointestinal cancers.";
RL   Cancer Res. 63:994-999(2003).
//
RX   PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675;
RA   Dutil, Julie
RA   Chen, Zhi-Hua
RA   Monteiro, Alvaro N.A.
RA   Teer, Jamie K.
RA   Eschrich, Steven A.
RT   "An interactive resource to probe genetic diversity and estimated
RT   ancestry in cancer cell lines.";
RL   Cancer Res. 79:1263-1273(2019).
//
RX   PubMed=25926053; DOI=10.1038/ncomms8002;
RA   Medico, Enzo
RA   Russo, Mariangela
RA   Picco, Gabriele
RA   Cancelliere, Carlotta
RA   Valtorta, Emanuele
RA   Corti, Giorgio
RA   Buscarino, Michela
RA   Isella, Claudio
RA   Lamba, Simona
RA   Martinoglio, Barbara
RA   Veronese, Silvio
RA   Siena, Salvatore
RA   Sartore-Bianchi, Andrea
RA   Beccuti, Marco
RA   Mottolese, Marcella
RA   Linnebacher, Michael
RA   Cordero, Francesca
RA   Di Nicolantonio, Federica
RA   Bardelli, Alberto
RT   "The molecular landscape of colorectal cancer cell lines unveils
RT   clinically actionable kinase targets.";
RL   Nat. Commun. 6:7002.1-7002.10(2015).
//
RX   PubMed=9328464; DOI=10.1093/hmg/6.12.2011;
RA   Kilian, Andrzej
RA   Bowtell, David D.L.
RA   Abud, Helen E.
RA   Hime, Gary R.
RA   Venter, Deon J.
RA   Keese, Paul K.
RA   Duncan, Emma L.
RA   Reddel, Roger Robert
RA   Jefferson, Richard Anthony
RT   "Isolation of a candidate human telomerase catalytic subunit gene,
RT   which reveals complex splicing patterns in different cell types.";
RL   Hum. Mol. Genet. 6:2011-2019(1997).
//
RX   PubMed=19378335; DOI=10.1002/ijc.24289;
RA   Zhang, Hui-Hua
RA   Walker, Francesca E.
RA   Kiflemariam, Sara
RA   Whitehead, Robert H.
RA   Williams, David
RA   Phillips, Wayne A.
RA   Mikeska, Thomas
RA   Dobrovic, Alexander
RA   Burgess, Antony Wilks
RT   "Selective inhibition of proliferation in colorectal carcinoma cell
RT   lines expressing mutant APC or activated B-Raf.";
RL   Int. J. Cancer 125:297-307(2009).
//
RX   PubMed=1427983; DOI=10.1038/icb.1992.30;
RA   Whitehead, Robert H.
RA   Zhang, Hui-Hua
RA   Hayward, Ian P.
RT   "Retention of tissue-specific phenotype in a panel of colon carcinoma
RT   cell lines: relationship to clinical correlates.";
RL   Immunol. Cell Biol. 70:227-236(1992).
//
RX   PubMed=22722830; DOI=10.1038/nature11156; PMCID=PMC3927413;
RA   Misale, Sandra
RA   Yaeger, Rona D.
RA   Hobor, Sebastijan
RA   Scala, Elisa
RA   Janakiraman, Manickam
RA   Liska, David
RA   Valtorta, Emanuele
RA   Schiavo, Roberta
RA   Buscarino, Michela
RA   Siravegna, Giulia
RA   Bencardino, Katia Bruna
RA   Cercek, Andrea
RA   Chen, Chin-Tung
RA   Veronese, Silvio
RA   Zanon, Carlo
RA   Sartore-Bianchi, Andrea
RA   Gambacorta, Marcello
RA   Gallicchio, Margherita
RA   Vakiani, Efsevia
RA   Boscaro, Valentina
RA   Medico, Enzo
RA   Weiser, Martin R.
RA   Siena, Salvatore
RA   Di Nicolantonio, Federica
RA   Solit, David B.
RA   Bardelli, Alberto
RT   "Emergence of KRAS mutations and acquired resistance to anti-EGFR
RT   therapy in colorectal cancer.";
RL   Nature 486:532-536(2012).
//
RX   PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192; PMCID=PMC2943514;
RA   Janakiraman, Manickam
RA   Vakiani, Efsevia
RA   Zeng, Zhao-Shi
RA   Pratilas, Christine A.
RA   Taylor, Barry Stephen
RA   Chitale, Dhananjay
RA   Halilovic, Ensar
RA   Wilson, Manda
RA   Huberman, Kety
RA   Ricarte Filho, Julio Cezar Marques
RA   Persaud, Yogindra
RA   Levine, Douglas A.
RA   Fagin, James Alexander
RA   Jhanwar, Suresh C.
RA   Mariadason, John M.
RA   Lash, Alex
RA   Ladanyi, Marc
RA   Saltz, Leonard B.
RA   Heguy, Adriana
RA   Paty, Philip B.
RA   Solit, David B.
RT   "Genomic and biological characterization of exon 4 KRAS mutations in
RT   human cancer.";
RL   Cancer Res. 70:5901-5911(2010).
//
RX   PubMed=23458625; DOI=10.1021/pr3010869;
RA   Fanayan, Susan
RA   Smith, Joshua T.
RA   Lee, Ling Y.
RA   Yan, Fang-Fei
RA   Snyder, Michael P.
RA   Hancock, William S.
RA   Nice, Edouard
RT   "Proteogenomic analysis of human colon carcinoma cell lines LIM1215,
RT   LIM1899, and LIM2405.";
RL   J. Proteome Res. 12:1732-1742(2013).
//
RX   PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457;
RA   Bazzocco, Sarah
RA   Dopeso, Higinio
RA   Carton-Garcia, Fernando
RA   Macaya, Irati
RA   Andretta, Elena
RA   Chionh, Fiona
RA   Rodrigues, Paulo
RA   Garrido, Miriam
RA   Alazzouzi, Hafid
RA   Nieto, Rocio
RA   Sanchez, Alex
RA   Schwartz, Simo Jr.
RA   Bilic, Josipa
RA   Mariadason, John M.
RA   Arango, Diego
RT   "Highly expressed genes in rapidly proliferating tumor cells as new
RT   targets for colorectal cancer treatment.";
RL   Clin. Cancer Res. 21:3695-3704(2015).
//
RX   PubMed=25623215; DOI=10.1158/1078-0432.CCR-14-2821;
RA   Arena, Sabrina
RA   Bellosillo, Beatriz
RA   Siravegna, Giulia
RA   Martinez, Alejandro
RA   Canadas, Israel
RA   Lazzari, Luca
RA   Ferruz, Noelia
RA   Russo, Mariangela
RA   Misale, Sandra
RA   Gonzalez, Iria
RA   Iglesias, Mar
RA   Gavilan, Elena
RA   Corti, Giorgio
RA   Hobor, Sebastijan
RA   Crisafulli, Giovanni
RA   Salido, Marta
RA   Sanchez, Juan
RA   Dalmases, Alba
RA   Bellmunt, Joaquim
RA   De Fabritiis, Gianni
RA   Rovira, Ana
RA   Di Nicolantonio, Federica
RA   Albanell, Joan
RA   Bardelli, Alberto
RA   Montagut, Clara
RT   "Emergence of multiple EGFR extracellular mutations during cetuximab
RT   treatment in colorectal cancer.";
RL   Clin. Cancer Res. 21:2157-2166(2015).
//
RX   PubMed=24295106; DOI=10.1021/pr400861m;
RA   Sethi, Manveen K.
RA   Thaysen-Andersen, Morten
RA   Smith, Joshua T.
RA   Baker, Mark Scott
RA   Packer, Nicolle H.
RA   Hancock, William S.
RA   Fanayan, Susan
RT   "Comparative N-glycan profiling of colorectal cancer cell lines
RT   reveals unique bisecting GlcNAc and alpha-2,3-linked sialic acid
RT   determinants are associated with membrane proteins of the more
RT   metastatic/aggressive cell lines.";
RL   J. Proteome Res. 13:277-288(2014).
//
RX   PubMed=9515795;
RA   Sparks, Andrew B.
RA   Morin, Patrice J.
RA   Vogelstein, Bert
RA   Kinzler, Kenneth Wayne
RT   "Mutational analysis of the APC/beta-catenin/Tcf pathway in colorectal
RT   cancer.";
RL   Cancer Res. 58:1130-1134(1998).
//
RX   PubMed=28854368; DOI=10.1016/j.celrep.2017.08.010; PMCID=PMC5583477;
RA   Roumeliotis, Theodoros I.
RA   Williams, Steven P.
RA   Goncalves, Emanuel
RA   Alsinet, Clara
RA   Del Castillo Velasco-Herrera, Martin
RA   Aben, Nanne
RA   Ghavidel, Fatemeh Zamanzad
RA   Michaut, Magali
RA   Schubert, Michael
RA   Price, Stacey
RA   Wright, James C.
RA   Yu, Lu
RA   Yang, Mi
RA   Dienstmann, Rodrigo
RA   Guinney, Justin H.
RA   Beltrao, Pedro
RA   Brazma, Alvis
RA   Pardo, Mercedes
RA   Stegle, Oliver
RA   Adams, David J.
RA   Wessels, Lodewyk F.A.
RA   Saez-Rodriguez, Julio
RA   McDermott, Ultan
RA   Choudhary, Jyoti S.
RT   "Genomic determinants of protein abundance variation in colorectal
RT   cancer cells.";
RL   Cell Rep. 20:2201-2214(2017).
//