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Cellosaurus PLB-985 (CVCL_2162)

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Cell line name PLB-985
Synonyms PLB 985; PLB985
Accession CVCL_2162
Resource Identification Initiative To cite this cell line use: PLB-985 (RRID:CVCL_2162)
Comments Problematic cell line: Contaminated. Shown to be a HL-60 derivative (PubMed=12592342; PubMed=20143388). Originally thought to originate from a 38 year old female patient with acute nonlymphocytic leukemia.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00258.
Population: Caucasian.
Doubling time: 35 hours (PubMed=25984343); ~24 hours (DSMZ=ACC-139).
Omics: Deep exome analysis.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene deletion; HGNC; 11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (DepMap=ACH-000034).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (DepMap=ACH-000034).
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North1.35
East Asian, South0
South Asian0
European, North69.55
European, South28.11
Disease Adult acute myeloid leukemia (NCIt: C9154)
Acute myeloid leukemia (ORDO: Orphanet_519)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0002 (HL-60)
Sex of cell Female
Age at sampling 36Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-139

Penta D10,11
Penta E13,14

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/p/cell-lines-detail-65.html

PubMed=3475136; DOI=10.1182/blood.V70.2.372.372
Tucker K.A., Lilly M.B., Heck L. Jr., Rado T.A.
Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity.
Blood 70:372-378(1987)

PubMed=8234321; DOI=10.1073/pnas.90.21.9832
Zhen L., King A.A.J., Xiao Y.-H., Chanock S.J., Orkin S.H., Dinauer M.C.
Gene targeting of X chromosome-linked chronic granulomatous disease locus in a human myeloid leukemia cell line and rescue by expression of recombinant gp91phox.
Proc. Natl. Acad. Sci. U.S.A. 90:9832-9836(1993)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=28287132; DOI=10.1038/srep44187
Wrona D., Siler U., Reichenbach J.
CRISPR/Cas9-generated p47(phox)-deficient cell line for chronic granulomatous disease gene therapy vector development.
Sci. Rep. 7:44187-44187(2017)

PubMed=29967760; DOI=10.3389/fonc.2018.00218
Kiffin R., Wiktorin H.G., Nilsson M.S., Aurelius J., Aydin E., Lenox B., Nilsson J.A., Stahlberg A., Thoren F.B., Hellstrand K., Martner A.
Anti-leukemic properties of histamine in monocytic leukemia: the role of NOX2.
Front. Oncol. 8:218.1-218.12(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cell line collections (Providers) DSMZ; ACC-139
Cell line databases/resources CLO; CLO_0008457
CLDB; cl3912
cancercelllines; CVCL_2162
Cell_Model_Passport; SIDM00811
DepMap; ACH-000034
DSMZCellDive; ACC-139
IGRhCellID; PLB985
Lonza; 281
Anatomy/cell type resources BTO; BTO:0002908
Biological sample resources BioSample; SAMN03151776
BioSample; SAMN10989584
Encyclopedic resources Wikidata; Q54947571
Experimental variables resources EFO; EFO_0002313
Gene expression databases ArrayExpress; E-MTAB-38
GEO; GSM1446750
GEO; GSM2690922
GEO; GSM2690923
GEO; GSM2690924
GEO; GSM2690925
GEO; GSM2690926
GEO; GSM2690927
GEO; GSM2690928
GEO; GSM2690929
GEO; GSM2690930
GEO; GSM2690931
GEO; GSM2690932
GEO; GSM2690933
Polymorphism and mutation databases Cosmic; 787475
Cosmic; 1089520
Progenetix; CVCL_2162
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number35