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Cellosaurus PLB-985 (CVCL_2162)

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Cell line name PLB-985
Synonyms PLB 985; PLB985
Accession CVCL_2162
Resource Identification Initiative To cite this cell line use: PLB-985 (RRID:CVCL_2162)
Comments Problematic cell line: Contaminated. Shown to be a HL-60 derivative (PubMed=12592342; PubMed=20143388). Originally thought to originate from a 38 year old female patient with acute nonlymphocytic leukemia.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00258.
Population: Caucasian.
Doubling time: 35 hours (PubMed=25984343); ~24 hours (DSMZ=ACC-139).
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; shRNA library screening.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (DepMap=ACH-000034).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (DepMap=ACH-000034).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.99
Native American0
East Asian, North1.35
East Asian, South0
South Asian0
European, North69.55
European, South28.11
Disease Adult acute myeloid leukemia with maturation (NCIt: C7961)
Acute myeloblastic leukemia with maturation (ORDO: Orphanet_98834)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0002 (HL-60)
Children:
CVCL_C3HD (PLB-985 CYBB KO)CVCL_C8HI (PLB-985 NCF1 DeltaGT)
Sex of cell Female
Age at sampling 36Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-139

Markers:
AmelogeninX
CSF1PO13,14
D2S133817
D3S135816
D5S81812
D7S82011,12
D8S117912
D13S3178,11
D16S53911
D18S5114,15
D19S43314
D21S1129,30
FGA22,24
Penta D10,11
Penta E13,14
TH017,8
TPOX8,11
vWA16

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/p/cell-lines-detail-65.html
Publications

PubMed=3475136; DOI=10.1182/blood.V70.2.372.372
Kent A. Tucker, Michael Brian Lilly, Louis Heck Jr., Thomas A. Rado;
Characterization of a new human diploid myeloid leukemia cell line (PLB-985) with granulocytic and monocytic differentiating capacity.
Blood 70:372-378(1987)

PubMed=8234321; DOI=10.1073/pnas.90.21.9832; PMCID=PMC47666
Ling Zhen, Alexandra A.J. King, Yong-Hong Xiao, Stephen Jacob Chanock, Stuart Holland Orkin, Mary C. Dinauer;
Gene targeting of X chromosome-linked chronic granulomatous disease locus in a human myeloid leukemia cell line and rescue by expression of recombinant gp91phox.
Proc. Natl. Acad. Sci. U.S.A. 90:9832-9836(1993)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Hans Gunther Drexler, Wilhelm Gerhard Dirks, Yoshinobu Matsuo, Roderick A.F. MacLeod;
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Glenn S. Cowley, Barbara A. Weir, Francisca Vazquez, Pablo Tamayo, Justine A. Scott, Scott Rusin, Alexandra East-Seletsky, Levi D. Ali, William F.J. Gerath, Sarah E. Pantel, Patrick Hall Lizotte ...Show all 40 authors... , Guo-Zhi Jiang, Jessica Hsiao, Aviad Tsherniak, Elizabeth Dwinell, Simon Aoyama, Michael Okamoto, William Harrington, Ellen T. Gelfand, Thomas M. Green, Mark J. Tomko, Shuba Gopal, Terence C. Wong, Hu-Bo Li, Sara Howell, Nicolas Stransky, Ted Liefeld, Dongkeun Jang, Jonathan Bistline, Barbara Hill Meyers, Scott Allen Armstrong, Kenneth Carl Anderson, Kimberly Stegmaier, Michael Reich, David Pellman, Jesse S. Boehm, Jill P. Mesirov, Todd Robert Golub, David E. Root, William Chun Hahn; Show fewer authors
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=28287132; DOI=10.1038/srep44187; PMCID=PMC5347011
Dominik Wrona, Ulrich Siler, Janine Reichenbach;
CRISPR/Cas9-generated p47(phox)-deficient cell line for chronic granulomatous disease gene therapy vector development.
Sci. Rep. 7:44187-44187(2017)

PubMed=29967760; DOI=10.3389/fonc.2018.00218; PMCID=PMC6015904
Roberta Kiffin, Hanna Grauers Wiktorin, Malin S. Nilsson, Johan Aurelius, Ebru Aydin, Brianna Lenox, Jonas A. Nilsson, Anders Stahlberg, Fredrik Bergh Thoren, Kristoffer Hellstrand, Anna Martner;
Anti-leukemic properties of histamine in monocytic leukemia: the role of NOX2.
Front. Oncol. 8:218.1-218.12(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=36982453; DOI=10.3390/ijms24065377; PMCID=PMC10049680
Rafal Skopek, Malgorzata Palusinska, Katarzyna Kaczor-Keller, Rafal Pingwara, Anna Papierniak-Wygladala, Tino Schenk, Slawomir Lewicki, Artur Zelent, Lukasz Szymanski;
Choosing the right cell line for acute myeloid leukemia (AML) research.
Int. J. Mol. Sci. 24:5377.1-5377.34(2023)

Cross-references
Cell line collections (Providers) DSMZ; ACC-139
Cell line databases/resources CLO; CLO_0008457
CLDB; cl3912
cancercelllines; CVCL_2162
Cell_Model_Passport; SIDM00811
DepMap; ACH-000034
DSMZCellDive; ACC-139
IGRhCellID; PLB985
Lonza; 281
Anatomy/cell type resources BTO; BTO_0002908
Biological sample resources BioSample; SAMN03151776
BioSample; SAMN10989584
Encyclopedic resources Wikidata; Q54947571
Experimental variables resources EFO; EFO_0002313
Gene expression databases ArrayExpress; E-MTAB-38
GEO; GSM1446750
GEO; GSM2690922
GEO; GSM2690923
GEO; GSM2690924
GEO; GSM2690925
GEO; GSM2690926
GEO; GSM2690927
GEO; GSM2690928
GEO; GSM2690929
GEO; GSM2690930
GEO; GSM2690931
GEO; GSM2690932
GEO; GSM2690933
Polymorphism and mutation databases Cosmic; 787475
Cosmic; 1089520
Progenetix; CVCL_2162
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number38