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Cellosaurus PLB-985 NCF1 DeltaGT (CVCL_C8HI)

[Text version]
Cell line name PLB-985 NCF1 DeltaGT
Accession CVCL_C8HI
Resource Identification Initiative To cite this cell line use: PLB-985 NCF1 DeltaGT (RRID:CVCL_C8HI)
Comments Problematic cell line: Contaminated. Parent cell line (PLB-985) was shown to be a HL-60 derivative.
Population: Caucasian.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7660; NCF1; Simple_edited; p.Tyr26Hisfs*26 (c.73_74GT[1]) (c.75_76delGT); ClinVar=VCV000002249; Zygosity=Homozygous; Note=By CRISPR/Cas9 (PubMed=28287132).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
Disease Adult acute myeloid leukemia with maturation (NCIt: C7961)
Acute myeloblastic leukemia with maturation (ORDO: Orphanet_98834)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2162 (PLB-985)
Sex of cell Female
Age at sampling 36Y
Category Cancer cell line
Publications

PubMed=28287132; DOI=10.1038/srep44187; PMCID=PMC5347011
Dominik Wrona, Ulrich Siler, Janine Reichenbach;
CRISPR/Cas9-generated p47(phox)-deficient cell line for chronic granulomatous disease gene therapy vector development.
Sci. Rep. 7:44187-44187(2017)

PubMed=33365312; DOI=10.3389/fcell.2020.608600; PMCID=PMC7751761
Jeremy Joly, Elodie Hudik, Sandrine Lecart, Dirk Roos, Paul Verkuijlen, Dominik Wrona, Ulrich Siler, Janine Reichenbach, Oliver Nusse, Sophie Dupre-Crochet;
Membrane dynamics and organization of the phagocyte NADPH oxidase in PLB-985 cells.
Front. Cell Dev. Biol. 8:608600.1-608600.10(2020)

Cross-references
Encyclopedic resources Wikidata; Q123033272
Entry history
Entry creation05-Oct-2023
Last entry update14-Aug-2025
Version number5