ID   PLB-985 NCF1 DeltaGT
AC   CVCL_C8HI
DR   Wikidata; Q123033272
RX   PubMed=28287132;
RX   PubMed=33365312;
CC   Problematic cell line: Contaminated. Parent cell line (PLB-985) was shown to be a HL-60 derivative.
CC   Population: Caucasian.
CC   Sequence variation: Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7660; NCF1; Simple_edited; p.Tyr26Hisfs*26 (c.73_74GT[1]) (c.75_76delGT); ClinVar=VCV000002249; Zygosity=Homozygous; Note=By CRISPR/Cas9 (PubMed=28287132).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
DI   NCIt; C9154; Adult acute myeloid leukemia
DI   NCIt; C154314; Autosomal recessive cytochrome b-positive chronic granulomatous disease type I
DI   ORDO; Orphanet_519; Acute myeloid leukemia
DI   ORDO; Orphanet_379; Chronic granulomatous disease
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_2162 ! PLB-985
SX   Female
AG   36Y
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 19-12-24; Version: 4
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RX   PubMed=28287132; DOI=10.1038/srep44187; PMCID=PMC5347011;
RA   Wrona D., Siler U., Reichenbach J.;
RT   "CRISPR/Cas9-generated p47(phox)-deficient cell line for chronic
RT   granulomatous disease gene therapy vector development.";
RL   Sci. Rep. 7:44187-44187(2017).
//
RX   PubMed=33365312; DOI=10.3389/fcell.2020.608600; PMCID=PMC7751761;
RA   Joly J., Hudik E., Lecart S., Roos D., Verkuijlen P., Wrona D.,
RA   Siler U., Reichenbach J., Nusse O., Dupre-Crochet S.;
RT   "Membrane dynamics and organization of the phagocyte NADPH oxidase in
RT   PLB-985 cells.";
RL   Front. Cell Dev. Biol. 8:608600.1-608600.10(2020).
//