ID   PLB-985
AC   CVCL_2162
SY   PLB 985; PLB985
DR   BTO; BTO:0002908
DR   CLO; CLO_0008457
DR   EFO; EFO_0002313
DR   CLDB; cl3912
DR   ArrayExpress; E-MTAB-38
DR   BioSample; SAMN03151776
DR   BioSample; SAMN10989584
DR   cancercelllines; CVCL_2162
DR   Cell_Model_Passport; SIDM00811
DR   Cosmic; 787475
DR   Cosmic; 1089520
DR   DepMap; ACH-000034
DR   DSMZ; ACC-139
DR   DSMZCellDive; ACC-139
DR   GEO; GSM1446750
DR   GEO; GSM2690922
DR   GEO; GSM2690923
DR   GEO; GSM2690924
DR   GEO; GSM2690925
DR   GEO; GSM2690926
DR   GEO; GSM2690927
DR   GEO; GSM2690928
DR   GEO; GSM2690929
DR   GEO; GSM2690930
DR   GEO; GSM2690931
DR   GEO; GSM2690932
DR   GEO; GSM2690933
DR   IGRhCellID; PLB985
DR   Lonza; 281
DR   Progenetix; CVCL_2162
DR   Wikidata; Q54947571
RX   PubMed=3475136;
RX   PubMed=8234321;
RX   PubMed=12592342;
RX   PubMed=20143388;
RX   PubMed=20215515;
RX   PubMed=25984343;
RX   PubMed=28287132;
RX   PubMed=29967760;
RX   PubMed=30894373;
RX   PubMed=31068700;
CC   Problematic cell line: Contaminated. Shown to be a HL-60 derivative (PubMed=12592342; PubMed=20143388). Originally thought to originate from a 38 year old female patient with acute nonlymphocytic leukemia.
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00258.
CC   Population: Caucasian.
CC   Doubling time: 35 hours (PubMed=25984343); ~24 hours (DSMZ=ACC-139).
CC   Sequence variation: Gene deletion; HGNC; 11998; TP53; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (DepMap=ACH-000034).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (DepMap=ACH-000034).
CC   Omics: Deep exome analysis.
CC   Omics: shRNA library screening.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Genome ancestry: African=0.99%; Native American=0%; East Asian, North=1.35%; East Asian, South=0%; South Asian=0%; European, North=69.55%; European, South=28.11% (PubMed=30894373).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
ST   Source(s): DSMZ=ACC-139
ST   Amelogenin: X
ST   CSF1PO: 13,14
ST   D13S317: 8,11
ST   D16S539: 11
ST   D18S51: 14,15
ST   D19S433: 14
ST   D21S11: 29,30
ST   D2S1338: 17
ST   D3S1358: 16
ST   D5S818: 12
ST   D7S820: 11,12
ST   D8S1179: 12
ST   FGA: 22,24
ST   Penta D: 10,11
ST   Penta E: 13,14
ST   TH01: 7,8
ST   TPOX: 8,11
ST   vWA: 16
DI   NCIt; C9154; Adult acute myeloid leukemia
DI   ORDO; Orphanet_519; Acute myeloid leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0002 ! HL-60
SX   Female
AG   36Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 02-05-24; Version: 35
RX   PubMed=3475136; DOI=10.1182/blood.V70.2.372.372;
RA   Tucker K.A., Lilly M.B., Heck L. Jr., Rado T.A.;
RT   "Characterization of a new human diploid myeloid leukemia cell line
RT   (PLB-985) with granulocytic and monocytic differentiating capacity.";
RL   Blood 70:372-378(1987).
RX   PubMed=8234321; DOI=10.1073/pnas.90.21.9832;
RA   Zhen L., King A.A.J., Xiao Y.-H., Chanock S.J., Orkin S.H., Dinauer M.C.;
RT   "Gene targeting of X chromosome-linked chronic granulomatous disease
RT   locus in a human myeloid leukemia cell line and rescue by expression
RT   of recombinant gp91phox.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:9832-9836(1993).
RX   PubMed=12592342; DOI=10.1038/sj.leu.2402799;
RA   Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.;
RT   "False leukemia-lymphoma cell lines: an update on over 500 cell
RT   lines.";
RL   Leukemia 17:416-426(2003).
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
RX   PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458;
RA   Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P.,
RA   Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J.,
RA   Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C.,
RA   Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J.,
RA   Haber D.A.;
RT   "A genome-wide screen for microdeletions reveals disruption of
RT   polarity complex genes in diverse human cancers.";
RL   Cancer Res. 70:2158-2164(2010).
RX   PubMed=25984343; DOI=10.1038/sdata.2014.35;
RA   Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S.,
RA   East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H.,
RA   Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M.,
RA   Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S.,
RA   Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D.,
RA   Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C.,
RA   Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P.,
RA   Golub T.R., Root D.E., Hahn W.C.;
RT   "Parallel genome-scale loss of function screens in 216 cancer cell
RT   lines for the identification of context-specific genetic
RT   dependencies.";
RL   Sci. Data 1:140035-140035(2014).
RX   PubMed=28287132; DOI=10.1038/srep44187;
RA   Wrona D., Siler U., Reichenbach J.;
RT   "CRISPR/Cas9-generated p47(phox)-deficient cell line for chronic
RT   granulomatous disease gene therapy vector development.";
RL   Sci. Rep. 7:44187-44187(2017).
RX   PubMed=29967760; DOI=10.3389/fonc.2018.00218;
RA   Kiffin R., Wiktorin H.G., Nilsson M.S., Aurelius J., Aydin E., Lenox B.,
RA   Nilsson J.A., Stahlberg A., Thoren F.B., Hellstrand K., Martner A.;
RT   "Anti-leukemic properties of histamine in monocytic leukemia: the role
RT   of NOX2.";
RL   Front. Oncol. 8:218.1-218.12(2018).
RX   PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747;
RA   Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.;
RT   "An interactive resource to probe genetic diversity and estimated
RT   ancestry in cancer cell lines.";
RL   Cancer Res. 79:1263-1273(2019).
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3;
RA   Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C.,
RA   McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X.,
RA   Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F.,
RA   Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R.,
RA   Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C.,
RA   Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A.,
RA   Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D.,
RA   Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L.,
RA   Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A.,
RA   Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D.,
RA   Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M.,
RA   Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R.,
RA   Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A.,
RA   Sellers W.R.;
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).