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Cellosaurus PK-15 (CVCL_2160)

[Text version]
Cell line name PK-15
Synonyms PK(15); PK (15); PK 15; PK15; Porcine Kidney-15
Accession CVCL_2160
Resource Identification Initiative To cite this cell line use: PK-15 (RRID:CVCL_2160)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: PK-15 from ATCC was found to be infected with porcine circovirus type 1 (PCV1) (PubMed=2686830; PubMed=4151202).
Doubling time: 12.1 +- 0.7 hours (PubMed=17889922); ~25 hours (DSMZ=ACC-640).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Hampshire.
Hierarchy Parent: CVCL_S287 (PK-2a)
CVCL_B0NA (PK-15/A11)CVCL_A5AA (PK-15(CD163))CVCL_JF19 (PK-15A)
CVCL_YB14 (PK-15S)CVCL_YE75 (PK15 PERV-inactivated clone 15)CVCL_YE76 (PK15 PERV-inactivated clone 20)
CVCL_YE77 (PK15 PERV-inactivated clone 29)CVCL_YE78 (PK15 PERV-inactivated clone 38)CVCL_A9EK (PK15-1A6)
CVCL_A9EH (PK15-1C8)CVCL_A9EI (PK15-1E5)CVCL_A9EJ (PK15-2F11)
CVCL_A9EL (PK15-3B1)CVCL_JF20 (PK15-A2)CVCL_A9EE (PK15-B1)
CVCL_XE83 (PK15-IL-2)CVCL_C0Z4 (PK15CD163)CVCL_W715 (PK15cl28/BS)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=PK15

Pirtle E.C.
Variation in the modal chromosome number of two PK-15 porcine kidney cell lines.
Am. J. Vet. Res. 27:747-749(1966)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X
Todaro G.J., Benveniste R.E., Lieber M.M., Sherr C.J.
Characterization of a type C virus released from the porcine cell line PK(15).
Virology 58:65-74(1974)

Tischer I., Rasch R., Tochtermann G.
Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines.
Zentralbl. Bakteriol. Orig. A. 226:153-167(1974)

Fulton R.W., Pearson N.J.
Interferon production by bovine tracheal organ cultures infected with bovid herpesvirus 1 strains.
Can. J. Comp. Med. 44:447-452(1980)

PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=3009166; DOI=10.1507/endocrj1954.32.819
Kinoshita Y., Fukase M., Takenaka M., Nakada M., Miyauchi A., Fujita T.
Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.
Endocrinol. Jpn. 32:819-828(1985)

Buonavoglia C., Falcone E., Pestalozza S., Iovane G., Rivero V.B.
Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.
Microbiologica 11:263-264(1988)

Dulac G.C., Afshar A.
Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.
Can. J. Vet. Res. 53:431-433(1989)

PubMed=17889922; DOI=10.1016/j.virol.2007.08.014
Zhu Y., Lau A.H.-L., Lau J.S.-K., Jia Q., Karuppannan A.K., Kwang J.H.-S.
Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line.
Virology 369:423-430(2007)

PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x
Ho C.S., Franzo-Romain M.H., Lee Y.J., Lee J.H., Smith D.M.
Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines.
Int. J. Immunogenet. 36:231-234(2009)

PubMed=19937495; DOI=10.1080/10495390903139950
Uebing-Czipura A.U., Dawson H.D., Rutherford M.S., Scherba G.
Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb.
Anim. Biotechnol. 20:186-215(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=24398227; DOI=10.1016/j.vetmic.2013.12.001
Lithgow P.E., Takamatsu H., Werling D., Dixon L.K., Chapman D.
Correlation of cell surface marker expression with African swine fever virus infection.
Vet. Microbiol. 168:413-419(2014)

PubMed=26456528; DOI=10.1126/science.aad1191
Yang L.-H., Guell M., Niu D., George H., Lesha E., Grishin D., Aach J., Shrock E., Xu W.-H., Poci J., Cortazio R., Wilkinson R.A., Fishman J.A., Church G.M.
Genome-wide inactivation of porcine endogenous retroviruses (PERVs).
Science 350:1101-1104(2015)

PubMed=31216434; DOI=10.1016/j.jviromet.2019.113682
Li Y.-Y., Wang Y.-J., Zhou X.-H., Zhang X.-K., Zhang X.-Y., Xia X.-L., Sun H.-C.
Generation of PK-15 cell lines highly permissive to porcine circovirus 2 infection by transposon-mediated interferon-gamma gene transfer.
J. Virol. Methods 271:113682.1-113682.6(2019)

PubMed=32258794; DOI=10.2478/jvetres-2020-0020
Yin M., Hu D.-F., Li P., Kong L.-Y., Ning H.-M., Yue F., Jiang J.-Q., Wang X.-N.
Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus.
J. Vet. Res. 64:9-14(2020)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)

Cell line collections (Providers) ATCC; CCL-33
ATCC; CRL-6593 - Discontinued
BCRC; 60057
BCRJ; 0355
CLS; 607426
KCB; KCB 2010002YJ
KCLB; 10033
RCB; RCB0534
Cell line databases/resources CLO; CLO_0008447
CLO; CLO_0008448
CLO; CLO_0050908
CLDB; cl3906
CLDB; cl3907
CLDB; cl3908
CCRID; 1101SWI-PUMC000385
DSMZCellDive; ACC-640
FCS-free; 42-15-47-1-8-9
Anatomy/cell type resources BTO; BTO:0001865
Encyclopedic resources Wikidata; Q54947433
Gene expression databases GEO; GSM422415
GEO; GSM422416
GEO; GSM422417
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number32