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Cellosaurus PK-15 (CVCL_2160)

[Text version]
Cell line name PK-15
Synonyms PK(15); PK (15); PK 15; PK15; Porcine Kidney-15
Accession CVCL_2160
Resource Identification Initiative To cite this cell line use: PK-15 (RRID:CVCL_2160)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: PK-15 from ATCC was found to be infected with porcine circovirus 1 (PCV1) (PubMed=2686830; PubMed=4151202).
Virology: Susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=15731278).
Doubling time: 12.1 +- 0.7 hours (PubMed=17889922); ~25 hours (DSMZ=ACC-640).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Hampshire; VBO=VBO_0001146.
Hierarchy Parent: CVCL_S287 (PK-2a)
Children:
CVCL_B0NA (PK-15/A11)CVCL_A5AA (PK-15(CD163))CVCL_JF19 (PK-15A)
CVCL_YB14 (PK-15S)CVCL_YE75 (PK15 PERV-inactivated clone 15)CVCL_YE76 (PK15 PERV-inactivated clone 20)
CVCL_YE77 (PK15 PERV-inactivated clone 29)CVCL_YE78 (PK15 PERV-inactivated clone 38)CVCL_A9EK (PK15-1A6)
CVCL_A9EH (PK15-1C8)CVCL_A9EI (PK15-1E5)CVCL_A9EJ (PK15-2F11)
CVCL_A9EL (PK15-3B1)CVCL_JF20 (PK15-A2)CVCL_A9EE (PK15-B1)
CVCL_JF21 (PK15-C1)CVCL_A9EF (PK15-IFNgRan)CVCL_A9EG (PK15-IFNgSB)
CVCL_XE83 (PK15-IL-2)CVCL_C0Z4 (PK15CD163)CVCL_W715 (PK15cl28/BS)
CVCL_C3VM (PK15S1)CVCL_C3VI (PK15S10)CVCL_E1AQ (Ubigene PK-15 ATG5 KO)
CVCL_E1AR (Ubigene PK-15 EEF1B2 KO)CVCL_E1AS (Ubigene PK-15 PGAM5 KO)CVCL_E1AT (Ubigene PK-15 RIPK3 KO)
CVCL_E1AU (Ubigene PK-15 SHMT1 KO)CVCL_E1AV (Ubigene PK-15 SHMT2 KO)CVCL_E1AW (Ubigene PK-15 VAMP2 KO)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=PK15
Publications

PubMed=5962858
Eugene C. Pirtle;
Variation in the modal chromosome number of two PK-15 porcine kidney cell lines.
Am. J. Vet. Res. 27:747-749(1966)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X
George J. Todaro, Raoul E. Benveniste, Michael M. Lieber, Charles J. Sherr;
Characterization of a type C virus released from the porcine cell line PK(15).
Virology 58:65-74(1974)

PubMed=4151202
Ilse Tischer, Renate Rasch, Gerta Tochtermann;
Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines.
Zentralbl. Bakteriol. Orig. A. 226:153-167(1974)

PubMed=6160900; PMCID=PMC1320103
Robert W. Fulton, Nancy J. Pearson;
Interferon production by bovine tracheal organ cultures infected with bovid herpesvirus 1 strains.
Can. J. Comp. Med. 44:447-452(1980)

PubMed=6988327; DOI=10.1007/BF02831503
Stephen James O'Brien, John E. Shannon, Mitchell H. Gail;
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=3009166; DOI=10.1507/endocrj1954.32.819
Yoshikazu Kinoshita, Masaaki Fukase, Mutsumi Takenaka, Masaki Nakada, Akimitsu Miyauchi, Takuo Fujita;
Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.
Endocrinol. Jpn. 32:819-828(1985)

PubMed=3173126
Canio Buonavoglia, Emiliana Falcone, Simonetta Pestalozza, Giuseppe Iovane, Vanessa B. Rivero;
Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.
Microbiologica 11:263-264(1988)

PubMed=2686830; PMCID=PMC1255571
Gilles C. Dulac, Ahmad Afshar;
Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.
Can. J. Vet. Res. 53:431-433(1989)

PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005; PMCID=PMC1075706
Eric C. Mossel, Cheng Huang, Krishna Narayanan, Shinji Makino, Robert B. Tesh, Clarence James Peters;
Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
J. Virol. 79:3846-3850(2005)

PubMed=17889922; DOI=10.1016/j.virol.2007.08.014
Yu Zhu, Adeline Hui-Ling Lau, Jennifer Siew-Kee Lau, Qiang Jia, Anbu K. Karuppannan, Jimmy H.-S. Kwang;
Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line.
Virology 369:423-430(2007)

PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x
Chak-Sum Ho, Megan Hope Franzo-Romain, Yu-Joo Lee, Jun-Heon Lee, Douglas M. Smith;
Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines.
Int. J. Immunogenet. 36:231-234(2009)

PubMed=19937495; DOI=10.1080/10495390903139950
Astrid Ulrike Uebing-Czipura, Harry D. Dawson, Mark S. Rutherford, Gail Scherba;
Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb.
Anim. Biotechnol. 20:186-215(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Axel Karger, Barbara Bettin, Matthias Lenk, Thomas Christoph Mettenleiter;
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=24398227; DOI=10.1016/j.vetmic.2013.12.001; PMCID=PMC3969584
Pamela E. Lithgow, Haru Takamatsu, Dirk Werling, Linda Kathleen Dixon, Dave Chapman;
Correlation of cell surface marker expression with African swine fever virus infection.
Vet. Microbiol. 168:413-419(2014)

PubMed=26456528; DOI=10.1126/science.aad1191
Lu-Han Yang, Marc Guell, Dong Niu, Haydy George, Emal Lesha, Dennis Grishin, John Aach, Ellen Shrock, Wei-Hong Xu, Jurgen Poci, Rebeca Cortazio ...Show all 14 authors... , Robert A. Wilkinson, Jay A. Fishman, George McDonald Church; Show fewer authors
Genome-wide inactivation of porcine endogenous retroviruses (PERVs).
Science 350:1101-1104(2015)

PubMed=31216434; DOI=10.1016/j.jviromet.2019.113682
Yang-Yang Li, Ya-Jie Wang, Xiao-Hui Zhou, Xiao-Kai Zhang, Xin-Yu Zhang, Xiao-Li Xia, Huai-Chang Sun;
Generation of PK-15 cell lines highly permissive to porcine circovirus 2 infection by transposon-mediated interferon-gamma gene transfer.
J. Virol. Methods 271:113682.1-113682.6(2019)

PubMed=32258794; DOI=10.2478/jvetres-2020-0020; PMCID=PMC7105985
Mei Yin, Dong-Fang Hu, Peng Li, Ling-Yun Kong, Hong-Mei Ning, Feng Yue, Jin-Qing Jiang, Xuan-Nian Wang;
Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus.
J. Vet. Res. 64:9-14(2020)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Oliver Lung, Rebecca C. Candlish, Michelle Nebroski, Peter Kruckiewicz, Cody Buchanan, Mariko Moniwa;
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657.1-21657.9(2021)

Cross-references
Cell line collections (Providers) ATCC; CCL-33
ATCC; CRL-6593 - Discontinued
BCRC; 60057
BCRJ; 0355
CCLV; CCLV-RIE 0005
CCTCC; GDC0061
CLS; 607426
DSMZ; ACC-640
IZSLER; BS CL 72
JCRB; JCRB9040
KCB; KCB 2010002YJ
KCLB; 10033
RCB; RCB0534
Cell line databases/resources CLO; CLO_0008447
CLO; CLO_0008448
CLO; CLO_0050908
CLDB; cl3906
CLDB; cl3907
CLDB; cl3908
CCRID; 1101SWI-PUMC000385
CCRID; 1102SWI-NIFDC00089
CCRID; 4201SWI-CCTCC00061
DSMZCellDive; ACC-640
FCS-free; 42-15-47-1-8-9
Anatomy/cell type resources BTO; BTO_0001865
Encyclopedic resources Wikidata; Q54947433
Gene expression databases GEO; GSM422415
GEO; GSM422416
GEO; GSM422417
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number37