Cellosaurus logo
expasy logo

Cellosaurus PK-15 (CVCL_2160)

[Text version]
Cell line name PK-15
Synonyms PK(15); PK (15); PK 15; PK15; Porcine Kidney-15
Accession CVCL_2160
Resource Identification Initiative To cite this cell line use: PK-15 (RRID:CVCL_2160)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: PK-15 from ATCC was found to be infected with porcine circovirus type 1 (PCV1) (PubMed=2686830; PubMed=4151202).
Virology: Susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=15731278).
Doubling time: 12.1 +- 0.7 hours (PubMed=17889922); ~25 hours (DSMZ=ACC-640).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Hampshire.
Hierarchy Parent: CVCL_S287 (PK-2a)
Children:
CVCL_B0NA (PK-15/A11)CVCL_A5AA (PK-15(CD163))CVCL_JF19 (PK-15A)
CVCL_YB14 (PK-15S)CVCL_YE75 (PK15 PERV-inactivated clone 15)CVCL_YE76 (PK15 PERV-inactivated clone 20)
CVCL_YE77 (PK15 PERV-inactivated clone 29)CVCL_YE78 (PK15 PERV-inactivated clone 38)CVCL_A9EK (PK15-1A6)
CVCL_A9EH (PK15-1C8)CVCL_A9EI (PK15-1E5)CVCL_A9EJ (PK15-2F11)
CVCL_A9EL (PK15-3B1)CVCL_JF20 (PK15-A2)CVCL_A9EE (PK15-B1)
CVCL_JF21 (PK15-C1)CVCL_A9EF (PK15-IFNgRan)CVCL_A9EG (PK15-IFNgSB)
CVCL_XE83 (PK15-IL-2)CVCL_C0Z4 (PK15CD163)CVCL_W715 (PK15cl28/BS)
CVCL_C3VM (PK15S1)CVCL_C3VI (PK15S10)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=PK15
Publications

PubMed=5962858
Pirtle E.C.
Variation in the modal chromosome number of two PK-15 porcine kidney cell lines.
Am. J. Vet. Res. 27:747-749(1966)

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X
Todaro G.J., Benveniste R.E., Lieber M.M., Sherr C.J.
Characterization of a type C virus released from the porcine cell line PK(15).
Virology 58:65-74(1974)

PubMed=4151202
Tischer I., Rasch R., Tochtermann G.
Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines.
Zentralbl. Bakteriol. Orig. A. 226:153-167(1974)

PubMed=6160900
Fulton R.W., Pearson N.J.
Interferon production by bovine tracheal organ cultures infected with bovid herpesvirus 1 strains.
Can. J. Comp. Med. 44:447-452(1980)

PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=3009166; DOI=10.1507/endocrj1954.32.819
Kinoshita Y., Fukase M., Takenaka M., Nakada M., Miyauchi A., Fujita T.
Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.
Endocrinol. Jpn. 32:819-828(1985)

PubMed=3173126
Buonavoglia C., Falcone E., Pestalozza S., Iovane G., Rivero V.B.
Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.
Microbiologica 11:263-264(1988)

PubMed=2686830
Dulac G.C., Afshar A.
Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.
Can. J. Vet. Res. 53:431-433(1989)

PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005
Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B., Peters C.J.
Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication.
J. Virol. 79:3846-3850(2005)

PubMed=17889922; DOI=10.1016/j.virol.2007.08.014
Zhu Y., Lau A.H.-L., Lau J.S.-K., Jia Q., Karuppannan A.K., Kwang J.H.-S.
Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line.
Virology 369:423-430(2007)

PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x
Ho C.S., Franzo-Romain M.H., Lee Y.J., Lee J.H., Smith D.M.
Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines.
Int. J. Immunogenet. 36:231-234(2009)

PubMed=19937495; DOI=10.1080/10495390903139950
Uebing-Czipura A.U., Dawson H.D., Rutherford M.S., Scherba G.
Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb.
Anim. Biotechnol. 20:186-215(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=24398227; DOI=10.1016/j.vetmic.2013.12.001
Lithgow P.E., Takamatsu H., Werling D., Dixon L.K., Chapman D.
Correlation of cell surface marker expression with African swine fever virus infection.
Vet. Microbiol. 168:413-419(2014)

PubMed=26456528; DOI=10.1126/science.aad1191
Yang L.-H., Guell M., Niu D., George H., Lesha E., Grishin D., Aach J., Shrock E., Xu W.-H., Poci J., Cortazio R., Wilkinson R.A., Fishman J.A., Church G.M.
Genome-wide inactivation of porcine endogenous retroviruses (PERVs).
Science 350:1101-1104(2015)

PubMed=31216434; DOI=10.1016/j.jviromet.2019.113682
Li Y.-Y., Wang Y.-J., Zhou X.-H., Zhang X.-K., Zhang X.-Y., Xia X.-L., Sun H.-C.
Generation of PK-15 cell lines highly permissive to porcine circovirus 2 infection by transposon-mediated interferon-gamma gene transfer.
J. Virol. Methods 271:113682.1-113682.6(2019)

PubMed=32258794; DOI=10.2478/jvetres-2020-0020
Yin M., Hu D.-F., Li P., Kong L.-Y., Ning H.-M., Yue F., Jiang J.-Q., Wang X.-N.
Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus.
J. Vet. Res. 64:9-14(2020)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)

Cross-references
Cell line collections (Providers) ATCC; CCL-33
ATCC; CRL-6593 - Discontinued
BCRC; 60057
BCRJ; 0355
CCLV; CCLV-RIE 0005
CCTCC; GDC0061
CLS; 607426
DSMZ; ACC-640
IZSLER; BS CL 72
JCRB; JCRB9040
KCB; KCB 2010002YJ
KCLB; 10033
RCB; RCB0534
Cell line databases/resources CLO; CLO_0008447
CLO; CLO_0008448
CLO; CLO_0050908
CLDB; cl3906
CLDB; cl3907
CLDB; cl3908
CCRID; 1101SWI-PUMC000385
CCRID; 1102SWI-NIFDC00089
CCRID; 4201SWI-CCTCC00061
DSMZCellDive; ACC-640
FCS-free; 42-15-47-1-8-9
Anatomy/cell type resources BTO; BTO:0001865
Encyclopedic resources Wikidata; Q54947433
Gene expression databases GEO; GSM422415
GEO; GSM422416
GEO; GSM422417
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number33