ID   PK-15
AC   CVCL_2160
SY   PK(15); PK (15); PK 15; PK15; Porcine Kidney-15
DR   BTO; BTO:0001865
DR   CLO; CLO_0008447
DR   CLO; CLO_0008448
DR   CLO; CLO_0050908
DR   CLDB; cl3906
DR   CLDB; cl3907
DR   CLDB; cl3908
DR   ATCC; CRL-6593
DR   BCRC; 60057
DR   BCRJ; 0355
DR   CCRID; 1101SWI-PUMC000385
DR   CCRID; 1102SWI-NIFDC00089
DR   CCRID; 4201SWI-CCTCC00061
DR   CLS; 607426
DR   DSMZ; ACC-640
DR   DSMZCellDive; ACC-640
DR   FCS-free; 42-15-47-1-8-9
DR   GEO; GSM422415
DR   GEO; GSM422416
DR   GEO; GSM422417
DR   KCB; KCB 2010002YJ
DR   KCLB; 10033
DR   RCB; RCB0534
DR   Wikidata; Q54947433
RX   DOI=10.1007/BF02618370;
RX   PubMed=2686830;
RX   PubMed=3009166;
RX   PubMed=3173126;
RX   PubMed=4132403;
RX   PubMed=4151202;
RX   PubMed=5962858;
RX   PubMed=6160900;
RX   PubMed=6988327;
RX   PubMed=15731278;
RX   PubMed=17889922;
RX   PubMed=19508353;
RX   PubMed=19941903;
RX   PubMed=19937495;
RX   PubMed=24398227;
RX   PubMed=26456528;
RX   PubMed=31216434;
RX   PubMed=32258794;
RX   PubMed=34737324;
CC   Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
CC   Virology: PK-15 from ATCC was found to be infected with porcine circovirus type 1 (PCV1) (PubMed=2686830; PubMed=4151202).
CC   Virology: Susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=15731278).
CC   Doubling time: 12.1 +- 0.7 hours (PubMed=17889922); ~25 hours (DSMZ=ACC-640).
CC   Omics: Transcriptome analysis by microarray.
CC   Discontinued: ATCC; CRL-6593; true.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Breed/subspecies: Hampshire.
OX   NCBI_TaxID=9823; ! Sus scrofa (Pig)
HI   CVCL_S287 ! PK-2a
SX   Male
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 33
RX   DOI=10.1007/BF02618370;
RA   Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.;
RT   "The animal cell culture collection.";
RL   In Vitro 5:1-16(1970).
RX   PubMed=2686830;
RA   Dulac G.C., Afshar A.;
RT   "Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and
RT   evidence of antibodies to circovirus in Canadian pigs.";
RL   Can. J. Vet. Res. 53:431-433(1989).
RX   PubMed=3009166; DOI=10.1507/endocrj1954.32.819;
RA   Kinoshita Y., Fukase M., Takenaka M., Nakada M., Miyauchi A.,
RA   Fujita T.;
RT   "Calcitonin-responsive clonal cell line from porcine kidney (PK(15))
RT   in serum-free medium.";
RL   Endocrinol. Jpn. 32:819-828(1985).
RX   PubMed=3173126;
RA   Buonavoglia C., Falcone E., Pestalozza S., Iovane G., Rivero V.B.;
RT   "Susceptibility of a minipig kidney cell line (MPK) to hog cholera
RT   virus.";
RL   Microbiologica 11:263-264(1988).
RX   PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X;
RA   Todaro G.J., Benveniste R.E., Lieber M.M., Sherr C.J.;
RT   "Characterization of a type C virus released from the porcine cell
RT   line PK(15).";
RL   Virology 58:65-74(1974).
RX   PubMed=4151202;
RA   Tischer I., Rasch R., Tochtermann G.;
RT   "Characterization of papovavirus-and picornavirus-like particles in
RT   permanent pig kidney cell lines.";
RL   Zentralbl. Bakteriol. Orig. A. 226:153-167(1974).
RX   PubMed=5962858;
RA   Pirtle E.C.;
RT   "Variation in the modal chromosome number of two PK-15 porcine kidney
RT   cell lines.";
RL   Am. J. Vet. Res. 27:747-749(1966).
RX   PubMed=6160900;
RA   Fulton R.W., Pearson N.J.;
RT   "Interferon production by bovine tracheal organ cultures infected with
RT   bovid herpesvirus 1 strains.";
RL   Can. J. Comp. Med. 44:447-452(1980).
RX   PubMed=6988327; DOI=10.1007/BF02831503;
RA   O'Brien S.J., Shannon J.E., Gail M.H.;
RT   "A molecular approach to the identification and individualization of
RT   human and animal cells in culture: isozyme and allozyme genetic
RT   signatures.";
RL   In Vitro 16:119-135(1980).
RX   PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005;
RA   Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B.,
RA   Peters C.J.;
RT   "Exogenous ACE2 expression allows refractory cell lines to support
RT   severe acute respiratory syndrome coronavirus replication.";
RL   J. Virol. 79:3846-3850(2005).
RX   PubMed=17889922; DOI=10.1016/j.virol.2007.08.014;
RA   Zhu Y., Lau A.H.-L., Lau J.S.-K., Jia Q., Karuppannan A.K.,
RA   Kwang J.H.-S.;
RT   "Enhanced replication of porcine circovirus type 2 (PCV2) in a
RT   homogeneous subpopulation of PK15 cell line.";
RL   Virology 369:423-430(2007).
RX   PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x;
RA   Ho C.S., Franzo-Romain M.H., Lee Y.J., Lee J.H., Smith D.M.;
RT   "Sequence-based characterization of swine leucocyte antigen alleles in
RT   commercially available porcine cell lines.";
RL   Int. J. Immunogenet. 36:231-234(2009).
RX   PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022;
RA   Karger A., Bettin B., Lenk M., Mettenleiter T.C.;
RT   "Rapid characterisation of cell cultures by matrix-assisted laser
RT   desorption/ionisation mass spectrometric typing.";
RL   J. Virol. Methods 164:116-121(2010).
RX   PubMed=19937495; DOI=10.1080/10495390903139950;
RA   Uebing-Czipura A.U., Dawson H.D., Rutherford M.S., Scherba G.;
RT   "Transcriptome profile and cytogenetic analysis of immortalized
RT   neuronally restricted progenitor cells derived from the porcine
RT   olfactory bulb.";
RL   Anim. Biotechnol. 20:186-215(2009).
RX   PubMed=24398227; DOI=10.1016/j.vetmic.2013.12.001;
RA   Lithgow P.E., Takamatsu H., Werling D., Dixon L.K., Chapman D.;
RT   "Correlation of cell surface marker expression with African swine
RT   fever virus infection.";
RL   Vet. Microbiol. 168:413-419(2014).
RX   PubMed=26456528; DOI=10.1126/science.aad1191;
RA   Yang L.-H., Guell M., Niu D., George H., Lesha E., Grishin D., Aach J.,
RA   Shrock E., Xu W.-H., Poci J., Cortazio R., Wilkinson R.A., Fishman J.A.,
RA   Church G.M.;
RT   "Genome-wide inactivation of porcine endogenous retroviruses
RT   (PERVs).";
RL   Science 350:1101-1104(2015).
RX   PubMed=31216434; DOI=10.1016/j.jviromet.2019.113682;
RA   Li Y.-Y., Wang Y.-J., Zhou X.-H., Zhang X.-K., Zhang X.-Y.,
RA   Xia X.-L., Sun H.-C.;
RT   "Generation of PK-15 cell lines highly permissive to porcine
RT   circovirus 2 infection by transposon-mediated interferon-gamma gene
RT   transfer.";
RL   J. Virol. Methods 271:113682.1-113682.6(2019).
RX   PubMed=32258794; DOI=10.2478/jvetres-2020-0020;
RA   Yin M., Hu D.-F., Li P., Kong L.-Y., Ning H.-M., Yue F., Jiang J.-Q.,
RA   Wang X.-N.;
RT   "Cloning and identification of PK15 cells for enhanced replication of
RT   classical swine fever virus.";
RL   J. Vet. Res. 64:9-14(2020).
RX   PubMed=34737324; DOI=10.1038/s41598-021-00779-5;
RA   Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C.,
RA   Moniwa M.;
RT   "High-throughput sequencing for species authentication and
RT   contamination detection of 63 cell lines.";
RL   Sci. Rep. 11:21657-21657(2021).