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Cellosaurus HuT 78 (CVCL_0337)

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Cell line name HuT 78
Synonyms Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78; HUT78; NCI-H78
Accession CVCL_0337
Resource Identification Initiative To cite this cell line use: HuT 78 (RRID:CVCL_0337)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Population: Caucasian.
Doubling time: 26 hours (PubMed=6244013); ~65 hours (ATCC=TIB-161); 26 hours (HIVReagentProgram=ARP-89).
Omics: Deep exome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Virome analysis using RNAseq.
Anecdotal: For a number of years it was not known that H9 used by Gallo's lab to isolate HIV-1 (HTLV-III) was in fact a clone of Hut 78 from Minna's lab (PubMed=2567177). This created a controversy between the labs of Minna and Gallo (PubMed=2193399).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Gene deletion; HGNC; 7208; MPDZ; Zygosity=Homozygous (PubMed=20215515).
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (PubMed=12068308; DepMap=ACH-000509).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Homozygous (PubMed=2144611; DepMap=ACH-000509).
HLA typing Source: PubMed=7707540
Class II
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0.76
East Asian, North2.56
East Asian, South0
South Asian0.32
European, North68.01
European, South28.04
Disease Sezary syndrome (NCIt: C3366)
Sezary syndrome (ORDO: Orphanet_3162)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_1240 (H9)CVCL_D3XT (HuT 78/HIV-1 FRE-3 clone B4A)CVCL_D3XU (HuT 78/HIV-1 FRE-3 clone C9A)
CVCL_D3XV (HuT 78/HIV-1 FRE-3 clone D5A)CVCL_D3XW (HuT 78/HIV-1 FRE-3 clone E2D)CVCL_X617 (HUT 78/HIV-1SF2)
CVCL_X682 (HUT 78/HIV-2D194)CVCL_X683 (HUT 78/HIV-2D205)CVCL_X611 (HUT 78/SIV-BK28)
CVCL_X616 (HUT 78/SIV-BK44)CVCL_D3XX (HuT 78/SIV/Mne clone E11S)CVCL_X609 (HUT 78/SIVmac251)
CVCL_0325 (HuT78B1)CVCL_9472 (PM1)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): ATCC=TIB-161; CCRID; CLS=300338; PubMed=11416159; PubMed=25877200; RCB=RCB1934; TKG=TKG 0375

D7S8208 (PubMed=25877200)
8,11 (ATCC=TIB-161; CCRID; CLS=300338; RCB=RCB1934; TKG=TKG 0375)
Penta D9
Penta E13,15

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-70.html

PubMed=6244013; DOI=10.1182/blood.V55.3.409.409
Gazdar A.F., Carney D.N., Bunn P.A. Jr., Russell E.K., Jaffe E.S., Schechter G.P., Guccion J.G.
Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.
Blood 55:409-417(1980)

PubMed=6975346; DOI=10.1084/jem.154.5.1403
Gootenberg J.E., Ruscetti F.W., Mier J.W., Gazdar A.F., Gallo R.C.
Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.
J. Exp. Med. 154:1403-1418(1981)

PubMed=6954533; DOI=10.1073/pnas.79.7.2194
Westin E.H., Gallo R.C., Arya S.K., Eva A., Souza L.M., Baluda M.A., Aaronson S.A., Wong-Staal F.
Differential expression of the amv gene in human hematopoietic cells.
Proc. Natl. Acad. Sci. U.S.A. 79:2194-2198(1982)

PubMed=6151082; DOI=10.1016/S0140-6736(84)91666-0
Popovic M., Read-Connole E.L., Gallo R.C.
T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III.
Lancet 324:1472-1473(1984)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C.
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=3495441; DOI=10.1002/eji.1830170503
Kanowith-Klein S., Saxon A., Uittenbogaart C.H.
Constitutive production of B cell differentiation factor-like activity by human T and B cell lines.
Eur. J. Immunol. 17:593-598(1987)

PubMed=2567177; DOI=10.1089/aid.1989.5.253
Mann D.L., O'Brien S.J., Gilbert D.A., Reid Y.A., Popovic M., Read-Connole E.L., Gallo R.C., Gazdar A.F.
Origin of the HIV-susceptible human CD4+ cell line H9.
AIDS Res. Hum. Retroviruses 5:253-255(1989)

PubMed=2144611; DOI=10.1128/mcb.10.10.5502-5509.1990
Cheng J., Haas M.
Frequent mutations in the p53 tumor suppressor gene in human leukemia T-cell lines.
Mol. Cell. Biol. 10:5502-5509(1990)

PubMed=2193399; DOI=10.1126/science.2193399
Rubinstein E.
The untold story of HUT78.
Science 248:1499-1507(1990)

Heyman M., Grander D., Brondum-Nielsen K., Cederblad B., Liu Y., Xu B., Einhorn S.
Interferon system defects in malignant T-cells.
Leukemia 8:425-434(1994)

PubMed=7707540; DOI=10.1128/JVI.69.5.3117-3124.1995
Arthur L.O., Bess J.W. Jr., Urban R.G., Strominger J.L., Morton W.R., Mann D.L., Henderson L.E., Benveniste R.E.
Macaques immunized with HLA-DR are protected from challenge with simian immunodeficiency virus.
J. Virol. 69:3117-3124(1995)

PubMed=8806089; DOI=10.1002/jcb.240630502
Gazdar A.F., Minna J.D.
NCI series of cell lines: an historical perspective.
J. Cell. Biochem. 63 Suppl. 24:1-11(1996)

PubMed=8806090; DOI=10.1002/jcb.240630503
Bunn P.A. Jr., Foss F.M.
T-cell lymphoma cell lines (HUT102 and HUT78) established at the National Cancer Institute: history and importance to understanding the biology, clinical features, and therapy of cutaneous T-cell lymphomas (CTCL) and adult T-cell leukemia-lymphomas (ATLL).
J. Cell. Biochem. 63 Suppl. 24:12-23(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10570904; DOI=10.1007/s100380050178
Ariyama Y., Mori T., Shinomiya T., Sakabe T., Fukuda Y., Kanamaru A., Yamada Y., Isobe M., Seto M., Nakamura Y., Inazawa J.
Chromosomal imbalances in adult T-cell leukemia revealed by comparative genomic hybridization: gains at 14q32 and 2p16-22 in cell lines.
J. Hum. Genet. 44:357-363(1999)

Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25355872; DOI=10.1128/JVI.02570-14
Cao S.-B., Strong M.J., Wang X., Moss W.N., Concha M., Lin Z., O'Grady T., Baddoo M., Fewell C., Renne R., Flemington E.K.
High-throughput RNA sequencing-based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project.
J. Virol. 89:713-729(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cell line collections (Providers) ATCC; TIB-161
BCRC; 60522
BCRJ; 0370
CLS; 300338
ECACC; 88041901
HIVReagentProgram; ARP-89
KCLB; 90078
NCBI_Iran; C185
RCB; RCB1934
TKG; TKG 0375
Cell line databases/resources CLO; CLO_0004304
CLO; CLO_0051010
MCCL; MCC:0000233
CLDB; cl1778
CLDB; cl1779
cancercelllines; CVCL_0337
CCRID; 1101HUM-PUMC000190
Cell_Model_Passport; SIDM00851
CGH-DB; 9026-4
DepMap; ACH-000509
IGRhCellID; HuT78
Lonza; 813
TOKU-E; 1977
Anatomy/cell type resources BTO; BTO:0001945
Biological sample resources BioSample; SAMN01821566
BioSample; SAMN03471940
BioSample; SAMN03473203
BioSample; SAMN10987600
Chemistry resources ChEMBL-Cells; CHEMBL3307500
ChEMBL-Targets; CHEMBL613978
PharmacoDB; HuT78_647_2019
PubChem_Cell_line; CVCL_0337
Encyclopedic resources Wikidata; Q54896909
Experimental variables resources EFO; EFO_0002209
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
GEO; GSM887154
GEO; GSM888226
GEO; GSM1374569
Polymorphism and mutation databases Cosmic; 724829
Cosmic; 1551450
Cosmic; 2153070
Cosmic; 2402468
Cosmic; 2765583
IARC_TP53; 185
LiGeA; CCLE_696
Progenetix; CVCL_0337
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number39