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Cellosaurus HuT 78 (CVCL_0337)

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Cell line name HuT 78
Synonyms Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78; HUT78; NCI-H78
Accession CVCL_0337
Resource Identification Initiative To cite this cell line use: HuT 78 (RRID:CVCL_0337)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Population: Caucasian.
Doubling time: 26 hours (PubMed=6244013); ~65 hours (ATCC=TIB-161); 26 hours (BEI_Resources=ARP-89).
Omics: Genomics; Whole exome sequencing.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq virome analysis.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Anecdotal: For a number of years it was not known that H9 used by Gallo's lab to isolate HIV-1 (HTLV-III) was in fact a clone of Hut 78 from Minna's lab (PubMed=2567177). This created a controversy between the labs of Minna and Gallo (PubMed=2193399).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
HLA typing Source: PubMed=7707540
Class II
HLA-DRDRB1*04:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.32
Native American0.76
East Asian, North2.56
East Asian, South0
South Asian0.32
European, North68.01
European, South28.04
Disease Sezary syndrome (NCIt: C3366)
Sezary syndrome (ORDO: Orphanet_3162)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_1240 (H9)CVCL_D3XT (HuT 78/HIV-1 FRE-3 clone B4A)CVCL_D3XU (HuT 78/HIV-1 FRE-3 clone C9A)
CVCL_D3XV (HuT 78/HIV-1 FRE-3 clone D5A)CVCL_D3XW (HuT 78/HIV-1 FRE-3 clone E2D)CVCL_X617 (HUT 78/HIV-1SF2)
CVCL_X682 (HUT 78/HIV-2D194)CVCL_X683 (HUT 78/HIV-2D205)CVCL_X611 (HUT 78/SIV-BK28)
CVCL_X616 (HUT 78/SIV-BK44)CVCL_D3XX (HuT 78/SIV/Mne clone E11S)CVCL_X609 (HUT 78/SIVmac251)
CVCL_0325 (HuT78B1)CVCL_9472 (PM1)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): ATCC=TIB-161; CCRID; CLS=300338; PubMed=11416159; PubMed=25877200; RCB=RCB1934; TKG=TKG 0375

Markers:
AmelogeninX,Y
CSF1PO11,12
D2S133820,25
D3S135815,16
D5S81811,12
D7S8208 (PubMed=25877200)
8,11 (ATCC=TIB-161; CCRID; CLS=300338; RCB=RCB1934; TKG=TKG 0375)
D8S117912,14
D13S3178,12
D16S53911,12
D18S5118
D19S43314
D21S1130
FGA21,25
Penta D9
Penta E13,15
TH018,9
TPOX8,9
vWA14,15

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-70.html
Provider; NIBSC; 0002; http://www.nibsc.org/Default.aspx?pageid=597&id=0002&theme=default
Publications

PubMed=6244013; DOI=10.1182/blood.V55.3.409.409
Adi F. Gazdar, Desmond Niall Carney, Paul A. Bunn Jr., Edward K. Russell, Elaine Sarkin Jaffe, Geraldine P. Schechter, John G. Guccion;
Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.
Blood 55:409-417(1980)

PubMed=6975346; DOI=10.1084/jem.154.5.1403; PMCID=PMC2186515
Joseph Eric Gootenberg, Francis W. Ruscetti, James W. Mier, Adi F. Gazdar, Robert Charles Gallo;
Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.
J. Exp. Med. 154:1403-1418(1981)

PubMed=6954533; DOI=10.1073/pnas.79.7.2194; PMCID=PMC346157
Eric H. Westin, Robert Charles Gallo, Suresh K. Arya, Alessandra Eva, Lawrence M. Souza, Marcel Albert Baluda, Stuart A. Aaronson, Flossie Wong-Staal;
Differential expression of the amv gene in human hematopoietic cells.
Proc. Natl. Acad. Sci. U.S.A. 79:2194-2198(1982)

PubMed=6151082; DOI=10.1016/S0140-6736(84)91666-0
Mikulas Popovic, Elizabeth Lee Read-Connole, Robert Charles Gallo;
T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III.
Lancet 324:1472-1473(1984)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 Carboxylic esterase.
Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Hans Gunther Drexler, Gerhard Gaedicke, Jun Minowada;
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)

PubMed=3495441; DOI=10.1002/eji.1830170503
Susan Kanowith-Klein, Andrew Saxon, Christel H. Uittenbogaart;
Constitutive production of B cell differentiation factor-like activity by human T and B cell lines.
Eur. J. Immunol. 17:593-598(1987)

PubMed=2567177; DOI=10.1089/aid.1989.5.253
Dean L. Mann, Stephen James O'Brien, Dennis A. Gilbert, Yvonne A. Reid, Mikulas Popovic, Elizabeth Lee Read-Connole, Robert Charles Gallo, Adi F. Gazdar;
Origin of the HIV-susceptible human CD4+ cell line H9.
AIDS Res. Hum. Retroviruses 5:253-255(1989)

PubMed=2144611; DOI=10.1128/mcb.10.10.5502-5509.1990; PMCID=PMC361264
Jian Cheng, Martin Haas;
Frequent mutations in the p53 tumor suppressor gene in human leukemia T-cell lines.
Mol. Cell. Biol. 10:5502-5509(1990)

PubMed=2193399; DOI=10.1126/science.2193399
Eric Rubinstein;
The untold story of HUT78.
Science 248:1499-1507(1990)

PubMed=8127147
Mats Heyman, Dan Grander, Karen Brondum-Nielsen, Britta Cederblad, Yie Liu, Bo Xu, Stefan Einhorn;
Interferon system defects in malignant T-cells.
Leukemia 8:425-434(1994)

PubMed=7707540; DOI=10.1128/JVI.69.5.3117-3124.1995; PMCID=PMC189013
Larry O. Arthur, Julian W. Bess Jr., Robert G. Urban, Jack Leonard Strominger, William R. Morton, Dean L. Mann, Louis E. Henderson, Raoul E. Benveniste;
Macaques immunized with HLA-DR are protected from challenge with simian immunodeficiency virus.
J. Virol. 69:3117-3124(1995)

PubMed=8806089; DOI=10.1002/jcb.240630502
Adi F. Gazdar, John D. Minna;
NCI series of cell lines: an historical perspective.
J. Cell. Biochem. 63 Suppl. 24:1-11(1996)

PubMed=8806090; DOI=10.1002/jcb.240630503
Paul A. Bunn Jr., Francine M. Foss;
T-cell lymphoma cell lines (HUT102 and HUT78) established at the National Cancer Institute: history and importance to understanding the biology, clinical features, and therapy of cutaneous T-cell lymphomas (CTCL) and adult T-cell leukemia-lymphomas (ATLL).
J. Cell. Biochem. Suppl. 24:12-23(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505
Ruby M. Phelps, Bruce E. Johnson, Daniel C. Ihde, Adi F. Gazdar, David P. Carbone, Patrick R. McClintock, Ritva Ilona Linnoila, Mary J. Matthews, Paul A. Bunn Jr., Desmond Niall Carney, John D. Minna, James L. Mulshine;
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. Suppl. 24:32-91(1996)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886
Jun Takizawa, Ritsuro Suzuki, Hiroyuki Kuroda, Atae Utsunomiya, Yoshitoyo Kagami, Tatsuroh Joh, Yoshifusa Aizawa, Ryuzo Ueda, Masao Seto;
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10570904; DOI=10.1007/s100380050178
Yoji Ariyama, Toshiki Mori, Takashi Shinomiya, Tomoya Sakabe, Yoji Fukuda, Akihisa Kanamaru, Yasuaki Yamada, Masaharu Isobe, Masao Seto, Yusuke Nakamura, Johji Inazawa;
Chromosomal imbalances in adult T-cell leukemia revealed by comparative genomic hybridization: gains at 14q32 and 2p16-22 in cell lines.
J. Hum. Genet. 44:357-363(1999)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland, Maureen Harrison, Arvind K. Virmani ...Show all 16 authors... , Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=12068308; DOI=10.1038/nature00766
Helen R. Davies, Graham Robert Bignell, Charles Cox, Philip J. Stephens, Sarah Edkins, Sheila Clegg, Jon W. Teague, Hayley Woffendin, Mathew J. Garnett, William Bottomley, Neil Davis, Ed Dicks, Rebecca Ewing ...Show all 52 authors... , Yvonne Floyd, Kristian Gray, Sarah Hall, Rachel Hawes, Jaime Hughes, Vivian Kosmidou, Andrew Menzies, Catherine Mould, Adrian Parker, Claire Stevens, Stephen Watt, Steven Hooper, Rebecca Wilson, Hiran Jayatilake, Barry Austin Gusterson, Colin S. Cooper, Janet M. Shipley, Darren Hargrave, Kathy Pritchard-Jones, Norman J. Maitland, Georgia Chenevix-Trench, Gregory J. Riggins, Darell Doty Bigner, Giuseppe Palmieri, Antonio Cossu, Adrienne M. Flanagan, Andrew Nicholson, Judy W.C. Ho, Suet Yi Leung, Siu Tsan Yuen, Barbara L. Weber, Hilliard Foster Seigler, Timothy L. Darrow, Hugh F. Paterson, Richard Marais, Christopher John Marshall, Richard Wooster, Michael Rudolf Stratton, P. Andrew Futreal; Show fewer authors
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera, Rui Wang, Angela Tam ...Show all 22 authors... , Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy, Manway Liu, Lauren Murray ...Show all 61 authors... , Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25355872; DOI=10.1128/JVI.02570-14; PMCID=PMC4301145
Su-Bing Cao, Michael J. Strong, Xia Wang, Walter N. Moss, Monica Concha, Zhen Lin, Tina O'Grady, Melody Baddoo, Claire Fewell, Rolf Renne, Erik K. Flemington;
High-throughput RNA sequencing-based virome analysis of 50 lymphoma cell lines from the Cancer Cell Line Encyclopedia project.
J. Virol. 89:713-729(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786
Kar-Tong Tan, Ling-Wen Ding, Qiao-Yang Sun, Zhen-Tang Lao, Wenwen Chien, Xi Ren, Jin-Fen Xiao, Xin-Yi Loh, Liang Xu, Michael Lill, Anand Mayakonda, De-Chen Lin, Henry He Yang ...Show all 14 authors... , Harold Phillip Koeffler; Show fewer authors
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940.1-940.13(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu, Alexander Y. Andreev-Drakhlin, Jaegil Kim ...Show all 68 authors... , Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections (Providers) ATCC; CRM-TIB-161
ATCC; TIB-161
BCRC; 60522
BCRJ; 0370
BEI_Resources; ARP-89
CCTCC; GDC0307
CLS; 300338
ECACC; 88041901
KCLB; 90078
NCBI_Iran; C185
RCB; RCB1934
TKG; TKG 0375
Cell line databases/resources CLO; CLO_0004304
CLO; CLO_0051010
MCCL; MCC:0000233
CLDB; cl1778
CLDB; cl1779
cancercelllines; CVCL_0337
CCRID; 1101HUM-PUMC000190
CCRID; 3101HUMTCHu206
Cell_Model_Passport; SIDM00851
CGH-DB; 9026-4
DepMap; ACH-000509
IGRhCellID; HuT78
LINCS_LDP; LCL-2005
Lonza; 813
TOKU-E; 1977
Anatomy/cell type resources BTO; BTO_0001945
Biological sample resources BioSample; SAMN01821566
BioSample; SAMN03471940
BioSample; SAMN03473203
BioSample; SAMN10987600
Chemistry resources ChEMBL-Cells; CHEMBL3307500
ChEMBL-Targets; CHEMBL613978
PharmacoDB; HuT78_647_2019
PubChem_Cell_line; CVCL_0337
Encyclopedic resources Wikidata; Q54896909
Experimental variables resources EFO; EFO_0002209
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
GEO; GSM887154
GEO; GSM888226
GEO; GSM1374569
Polymorphism and mutation databases Cosmic; 724829
Cosmic; 1551450
Cosmic; 2153070
Cosmic; 2402468
Cosmic; 2765583
IARC_TP53; 185
LiGeA; CCLE_696
Progenetix; CVCL_0337
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number42