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Cellosaurus FRTL-5 (CVCL_0265)

[Text version]
Cell line name FRTL-5
Synonyms FRTL 5; FRTL5; FRTL-5 Cl 2
Accession CVCL_0265
Resource Identification Initiative To cite this cell line use: FRTL-5 (RRID:CVCL_0265)
Comments Group: Patented cell line.
Group: Space-flown cell line (cellonaut).
Problematic cell line: Probably misclassified/misidentified. ECACC has discontinued this cell line because it does not display the non-diploid karyotype originally reported therefore its authenticity cannot be assured.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8305.
Doubling time: 34 hours (CelloPub=CLPUB00429); 30 hours (PubMed=3670314).
Anecdotal: Have been flown in space on the ISS in 2005 to study the effect of UVC radiation (PubMed=21087161).
Derived from site: In situ; Thyroid gland; UBERON=UBERON_0002046.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Fischer 344; RS=RS_0000215.
Hierarchy Parent: CVCL_5753 (FRTL)
Children:
CVCL_A6IN (5UD5)CVCL_UG77 (FRTL-5 AG1)CVCL_UG78 (FRTL-5 AG2)
CVCL_E5TV (FRTL-5 H-RAS(V12) clone V13)CVCL_E5TW (FRTL-5 H-RAS(V12) clone V26)CVCL_E5TX (FRTL-5 H-RAS(V12) clone V27)
CVCL_E5TY (FRTL-5 H-RAS(V12) clone V29)CVCL_E5TZ (FRTL-5 H-RAS(V12) clone V30)CVCL_E5U0 (FRTL-5 H-RAS(V12) clone V31)
CVCL_E5U1 (FRTL-5 H-RAS(V12) clone V32)CVCL_VG49 (FRTL-5 Miami)CVCL_E5U2 (FRTL-5 v-Ha-RAS clone H31)
CVCL_E5U3 (FRTL-5 v-Ha-RAS clone H35)CVCL_E5U4 (FRTL-5 v-Ha-RAS clone H45)CVCL_E5U5 (FRTL-5 v-Ha-RAS clone H6)
CVCL_E5U6 (FRTL-5 v-Ki-RAS clone K1)CVCL_E5U7 (FRTL-5 v-Ki-RAS clone K2)CVCL_E5U8 (FRTL-5 v-Ki-RAS clone K28)
CVCL_E5U9 (FRTL-5 v-Ki-RAS clone K37)CVCL_A6IJ (FRTL-5/T)
Sex of cell Sex unspecified
Age at sampling 6W
Category Spontaneously immortalized cell line
Publications

PubMed=6327870; DOI=10.1677/joe.0.1010269
Stephen P. Bidey, Luca Chiovato, Alan Day, Mark Turmaine, Reginald P. Gould, Roger Philip Ekins, Nicholas J. Marshall;
Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro bioassay system for thyrotrophin.
J. Endocrinol. 101:269-276(1984)

Patent=US4608341
Fransesco Saverio Ambesi-Impiombato;
Living, fast-growing thyroid cell strain, FRTL-5.
Patent number US4608341, 26-Aug-1986

PubMed=2868604; DOI=10.1530/acta.0.1110054
Maria Luisa Brandi, Carlo Maria Rotella, Roberto Zonefrati, Roberto Toccafondi, Salvatore M. Aloj;
Loss of adrenergic regulation of cAMP production in the FRTL-5 cell line.
Acta Endocrinol. 111:54-61(1986)

PubMed=3475907; DOI=10.1530/acta.0.114s242
Fransesco Saverio Ambesi-Impiombato, Giovanni Villone;
The FRTL-5 thyroid cell strain as a model for studies on thyroid cell growth.
Acta Endocrinol. Suppl. 281:242-245(1987)

PubMed=3670314; DOI=10.1128/mcb.7.9.3365-3370.1987; PMCID=PMC367981
Alfredo Fusco, Maria T. Berlingieri, Pier Paolo Di Fiore, Giuseppe Portella, Michele Grieco, Giancarlo Vecchio;
One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes.
Mol. Cell. Biol. 7:3365-3370(1987)

PubMed=2545492; DOI=10.1016/0303-7207(89)90020-8
Hideo Sugawa, Toru Mori, Hiroo Imura;
Establishment of 8-azaguanine-resistant mutants from rat thyroid cell line FRTL-5.
Mol. Cell. Endocrinol. 62:319-326(1989)

PubMed=2551628; DOI=10.1210/endo-125-4-1783
Xuan-Ping Pang, Jerome Marshall Hershman, Matt Chung, A. Eugene Pekary;
Characterization of tumor necrosis factor-alpha receptors in human and rat thyroid cells and regulation of the receptors by thyrotropin.
Endocrinology 125:1783-1788(1989)

PubMed=1694496; DOI=10.1210/endo-127-1-419
Ferry A. Ossendorp, Peter F. Bruning, Ed M.D. Schuuring, Jacqueline A.M. van den Brink, Daan van der Heide, Jan J.M. de Vijlder, Tjerk W.A. de Bruin;
Thyrotropin dependent and independent thyroid cell lines selected from FRTL-5 derived tumors grown in nude mice.
Endocrinology 127:419-430(1990)

CLPUB00429
Hans Gerber, Hans-Jakob Peter, Jurg Bosiger, Hugo Studer, Roman Drews, Duncan Craig Ferguson, Mark E. Peterson, David Victor Becker;
Different continuous cell lines from adenomatous feline goiters widely differ in morphologic, functional and growth parameters.
(In book chapter) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon, Amirav & Gross, Jack & Hennemann, Georg (eds.); pp.541-544; A. A. Balkema Publishers; Rotterdam; Netherlands (1991)

CLPUB00430
Hans-Jakob Peter, Hans Gerber, Hugo Studer, Peter Groscurth, Margita Zakarija;
Thyroid cell lines forming follicle-like lumina in vitro.
(In book chapter) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon, Amirav & Gross, Jack & Hennemann, Georg (eds.); pp.579-582; A. A. Balkema Publishers; Rotterdam; Netherlands (1991)

PubMed=1726925
Hans Gerber, Hans-Jakob Peter, Lars Asmis, Hugo Studer;
Thyroid cell lines in research on goitrogenesis.
Thyroidology 3:115-118(1991)

PubMed=1846098; DOI=10.1210/endo-128-1-211
Hans-Jakob Peter, Hans Gerber, Hugo Studer, Peter Groscurth, Margita Zakarija;
Comparison of FRTL-5 cell growth in vitro with that of xenotransplanted cells and the thyroid of the recipient mouse.
Endocrinology 128:211-219(1991)

PubMed=7836899; DOI=10.1677/joe.0.1430527
Sheila MacNeil, Donald S. Munro, Russell Anthony Metcalfe, S. Cotterell, Ludmila N. Ruban, Rose Davies, Anthony Peter Weetman;
An investigation of the ability of TSH and Graves' immunoglobulin G to increase intracellular calcium in human thyroid cells, rat FRTL-5 thyroid cells and eukaryotic cells transfected with the human TSH receptor.
J. Endocrinol. 143:527-540(1994)

PubMed=7912172; DOI=10.1111/j.1365-2265.1994.tb03017.x
Valdo P. Michelangeli, Donald S. Munro, Cathy W. Poon, Albert G. Frauman, Peter G. Colman;
Measurement of thyroid stimulating immunoglobulins in a new cell line transfected with a functional human TSH receptor (JPO9 cells), compared with an assay using FRTL-5 cells.
Clin. Endocrinol. (Oxf.) 40:645-652(1994)

PubMed=8761378; DOI=10.1038/bjc.1996.409; PMCID=PMC2074668
Carla J.H. van der Kallen, J.H. Coes, Judith P. van Grafhorst, Ed M.D. Schuuring, Ferry A. Ossendorp, Joseph Helenus Hubert Thijssen, Marinus A. Blankenstein, Tjerk W.A. de Bruin;
Dissociation of thyrotropin receptor function and thyrotropin dependency in rat thyroid tumour cell lines derived from FRTL-5.
Br. J. Cancer 74:606-612(1996)

PubMed=9709917; DOI=10.1089/thy.1998.8.623
Vitomir Tasevski, Diana Benn, Greg Peters, Brian Luttrell, Ann Simpson;
The Fischer rat thyroid cell line FRTL-5 exhibits a nondiploid karyotype.
Thyroid 8:623-626(1998)

PubMed=20151827; DOI=10.1089/thy.2009.0171
Hichem Sebai, Sonia Hovsepian, Elodie Ristorcelli, Azedine Aouani, Dominique Lombardo, Guy Fayet;
Resveratrol increases iodide trapping in the rat thyroid cell line FRTL-5.
Thyroid 20:195-203(2010)

PubMed=21087161; DOI=10.1089/ast.2010.0461
Elisabetta Albi, Fransesco Saverio Ambesi-Impiombato, Maristella Villani, Ilaria De Pol, Renza Spelat, Remo Lazzarini, Giuseppina Perrella;
Thyroid cell growth: sphingomyelin metabolism as non-invasive marker for cell damage acquired during spaceflight.
Astrobiology 10:811-820(2010)

DOI=10.1128/mcb.00663-17
Alfredo Fusco, Maria T. Berlingieri, Pier Paolo Di Fiore, Giuseppe Portella, Michele Grieco, Giancarlo Vecchio;
Retraction for Fusco et al., 'One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes'.
Mol. Cell. Biol. 38:e00663.17.1-e00663.17.1(2018)

Cross-references
Cell line collections (Providers) CLS; 500407
ECACC; 91030711 - Discontinued
Cell line databases/resources CLO; CLO_0003396
MCCL; MCC:0000156
CLDB; cl1395
Anatomy/cell type resources BTO; BTO_0001875
Chemistry resources ChEMBL-Cells; CHEMBL3308088
ChEMBL-Targets; CHEMBL613879
PubChem_Cell_line; CVCL_0265
Encyclopedic resources Wikidata; Q54835180
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number29