ID   FRTL-5
AC   CVCL_0265
SY   FRTL 5; FRTL5; FRTL-5 Cl 2
DR   BTO; BTO_0001875
DR   CLO; CLO_0003396
DR   MCCL; MCC:0000156
DR   CLDB; cl1395
DR   ChEMBL-Cells; CHEMBL3308088
DR   ChEMBL-Targets; CHEMBL613879
DR   CLS; 500407
DR   ECACC; 91030711
DR   PubChem_Cell_line; CVCL_0265
DR   Wikidata; Q54835180
RX   CelloPub=CLPUB00429;
RX   CelloPub=CLPUB00430;
RX   DOI=10.1128/mcb.00663-17;
RX   Patent=US4608341;
RX   PubMed=1694496;
RX   PubMed=1726925;
RX   PubMed=1846098;
RX   PubMed=2545492;
RX   PubMed=2551628;
RX   PubMed=2868604;
RX   PubMed=3475907;
RX   PubMed=3670314;
RX   PubMed=6327870;
RX   PubMed=7836899;
RX   PubMed=7912172;
RX   PubMed=8761378;
RX   PubMed=9709917;
RX   PubMed=20151827;
RX   PubMed=21087161;
CC   Group: Patented cell line.
CC   Group: Space-flown cell line (cellonaut).
CC   Problematic cell line: Probably misclassified/misidentified. ECACC has discontinued this cell line because it does not display the non-diploid karyotype originally reported therefore its authenticity cannot be assured.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8305.
CC   Doubling time: 34 hours (CelloPub=CLPUB00429); 30 hours (PubMed=3670314).
CC   Anecdotal: Have been flown in space on the ISS in 2005 to study the effect of UVC radiation (PubMed=21087161).
CC   Discontinued: ECACC; 91030711; true.
CC   Derived from site: In situ; Thyroid gland; UBERON=UBERON_0002046.
CC   Breed/subspecies: Fischer 344; RS=RS_0000215.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_5753 ! FRTL
SX   Sex unspecified
AG   6W
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 29
CH   CVCL_A6IN ! 5UD5
CH   CVCL_UG77 ! FRTL-5 AG1
CH   CVCL_UG78 ! FRTL-5 AG2
CH   CVCL_E5TV ! FRTL-5 H-RAS(V12) clone V13
CH   CVCL_E5TW ! FRTL-5 H-RAS(V12) clone V26
CH   CVCL_E5TX ! FRTL-5 H-RAS(V12) clone V27
CH   CVCL_E5TY ! FRTL-5 H-RAS(V12) clone V29
CH   CVCL_E5TZ ! FRTL-5 H-RAS(V12) clone V30
CH   CVCL_E5U0 ! FRTL-5 H-RAS(V12) clone V31
CH   CVCL_E5U1 ! FRTL-5 H-RAS(V12) clone V32
CH   CVCL_VG49 ! FRTL-5 Miami
CH   CVCL_E5U2 ! FRTL-5 v-Ha-RAS clone H31
CH   CVCL_E5U3 ! FRTL-5 v-Ha-RAS clone H35
CH   CVCL_E5U4 ! FRTL-5 v-Ha-RAS clone H45
CH   CVCL_E5U5 ! FRTL-5 v-Ha-RAS clone H6
CH   CVCL_E5U6 ! FRTL-5 v-Ki-RAS clone K1
CH   CVCL_E5U7 ! FRTL-5 v-Ki-RAS clone K2
CH   CVCL_E5U8 ! FRTL-5 v-Ki-RAS clone K28
CH   CVCL_E5U9 ! FRTL-5 v-Ki-RAS clone K37
CH   CVCL_A6IJ ! FRTL-5/T
//
RX   PubMed=1694496; DOI=10.1210/endo-127-1-419;
RA   Ossendorp, Ferdinand Antonius
RA   Bruning, Peter F.
RA   Schuuring, Ed M.D.
RA   van den Brink, Jacqueline A.M.
RA   van der Heide, Daan
RA   de Vijlder, Johannes Josephus Marie
RA   de Bruin, Tjerk W.A.
RT   "Thyrotropin dependent and independent thyroid cell lines selected
RT   from FRTL-5 derived tumors grown in nude mice.";
RL   Endocrinology 127:419-430(1990).
//
RX   PubMed=3475907; DOI=10.1530/acta.0.114s242;
RA   Ambesi-Impiombato, Fransesco Saverio
RA   Villone, Giovanni
RT   "The FRTL-5 thyroid cell strain as a model for studies on thyroid cell
RT   growth.";
RL   Acta Endocrinol. Suppl. 281:242-245(1987).
//
RX   PubMed=3670314; DOI=10.1128/mcb.7.9.3365-3370.1987; PMCID=PMC367981;
RA   Fusco, Alfredo
RA   Berlingieri, Maria T.
RA   Di Fiore, Pier Paolo
RA   Portella, Giuseppe
RA   Grieco, Michele
RA   Vecchio, Giancarlo
RT   "One- and two-step transformations of rat thyroid epithelial cells by
RT   retroviral oncogenes.";
RL   Mol. Cell. Biol. 7:3365-3370(1987).
//
RX   PubMed=20151827; DOI=10.1089/thy.2009.0171;
RA   Sebai, Hichem
RA   Hovsepian, Sonia
RA   Ristorcelli, Elodie
RA   Aouani, Azedine
RA   Lombardo, Dominique
RA   Fayet, Guy
RT   "Resveratrol increases iodide trapping in the rat thyroid cell line
RT   FRTL-5.";
RL   Thyroid 20:195-203(2010).
//
RX   PubMed=2868604; DOI=10.1530/acta.0.1110054;
RA   Brandi, Maria Luisa
RA   Rotella, Carlo Maria
RA   Zonefrati, Roberto
RA   Toccafondi, Roberto
RA   Aloj, Salvatore M.
RT   "Loss of adrenergic regulation of cAMP production in the FRTL-5 cell
RT   line.";
RL   Acta Endocrinol. 111:54-61(1986).
//
RX   PubMed=7912172; DOI=10.1111/j.1365-2265.1994.tb03017.x;
RA   Michelangeli, Valdo P.
RA   Munro, Donald S.
RA   Poon, Cathy W.
RA   Frauman, Albert G.
RA   Colman, Peter G.
RT   "Measurement of thyroid stimulating immunoglobulins in a new cell line
RT   transfected with a functional human TSH receptor (JPO9 cells),
RT   compared with an assay using FRTL-5 cells.";
RL   Clin. Endocrinol. (Oxf.) 40:645-652(1994).
//
RX   CelloPub=CLPUB00430;
RA   Peter, Hans-Jakob
RA   Gerber, Hans
RA   Studer, Hugo
RA   Groscurth, Peter
RA   Zakarija, Margita
RT   "Thyroid cell lines forming follicle-like lumina in vitro.";
RL   (In book chapter) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon, Amirav & Gross, Jack & Hennemann, Georg (eds.); pp.579-582; A. A. Balkema Publishers; Rotterdam; Netherlands (1991).
//
RX   PubMed=21087161; DOI=10.1089/ast.2010.0461;
RA   Albi, Elisabetta
RA   Ambesi-Impiombato, Fransesco Saverio
RA   Villani, Maristella
RA   De Pol, Ilaria
RA   Spelat, Renza
RA   Lazzarini, Remo
RA   Perrella, Giuseppina
RT   "Thyroid cell growth: sphingomyelin metabolism as non-invasive marker
RT   for cell damage acquired during spaceflight.";
RL   Astrobiology 10:811-820(2010).
//
RX   PubMed=1726925;
RA   Gerber, Hans
RA   Peter, Hans-Jakob
RA   Asmis, Lars
RA   Studer, Hugo
RT   "Thyroid cell lines in research on goitrogenesis.";
RL   Thyroidology 3:115-118(1991).
//
RX   PubMed=1846098; DOI=10.1210/endo-128-1-211;
RA   Peter, Hans-Jakob
RA   Gerber, Hans
RA   Studer, Hugo
RA   Groscurth, Peter
RA   Zakarija, Margita
RT   "Comparison of FRTL-5 cell growth in vitro with that of
RT   xenotransplanted cells and the thyroid of the recipient mouse.";
RL   Endocrinology 128:211-219(1991).
//
RX   PubMed=2545492; DOI=10.1016/0303-7207(89)90020-8;
RA   Sugawa, Hideo
RA   Mori, Toru
RA   Imura, Hiroo
RT   "Establishment of 8-azaguanine-resistant mutants from rat thyroid cell
RT   line FRTL-5.";
RL   Mol. Cell. Endocrinol. 62:319-326(1989).
//
RX   CelloPub=CLPUB00429;
RA   Gerber, Hans
RA   Peter, Hans-Jakob
RA   Bosiger, Jurg
RA   Studer, Hugo
RA   Drews, Roman
RA   Ferguson, Duncan Craig
RA   Peterson, Mark E.
RA   Becker, David Victor
RT   "Different continuous cell lines from adenomatous feline goiters
RT   widely differ in morphologic, functional and growth parameters.";
RL   (In book chapter) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon, Amirav & Gross, Jack & Hennemann, Georg (eds.); pp.541-544; A. A. Balkema Publishers; Rotterdam; Netherlands (1991).
//
RX   PubMed=2551628; DOI=10.1210/endo-125-4-1783;
RA   Pang, Xuan-Ping
RA   Hershman, Jerome Marshall
RA   Chung, Matt
RA   Pekary, A. Eugene
RT   "Characterization of tumor necrosis factor-alpha receptors in human
RT   and rat thyroid cells and regulation of the receptors by
RT   thyrotropin.";
RL   Endocrinology 125:1783-1788(1989).
//
RX   PubMed=8761378; DOI=10.1038/bjc.1996.409; PMCID=PMC2074668;
RA   van der Kallen, Carla J.H.
RA   Coes, J.H.
RA   van Grafhorst, Judith P.
RA   Schuuring, Ed M.D.
RA   Ossendorp, Ferdinand Antonius
RA   Thijssen, Joseph Helenus Hubert
RA   Blankenstein, Marinus A.
RA   de Bruin, Tjerk W.A.
RT   "Dissociation of thyrotropin receptor function and thyrotropin
RT   dependency in rat thyroid tumour cell lines derived from FRTL-5.";
RL   Br. J. Cancer 74:606-612(1996).
//
RX   PubMed=6327870; DOI=10.1677/joe.0.1010269;
RA   Bidey, Stephen P.
RA   Chiovato, Luca
RA   Day, Alan
RA   Turmaine, Mark
RA   Gould, Reginald P.
RA   Ekins, Roger Philip
RA   Marshall, Nicholas J.
RT   "Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro
RT   bioassay system for thyrotrophin.";
RL   J. Endocrinol. 101:269-276(1984).
//
RX   DOI=10.1128/mcb.00663-17;
RA   Fusco, Alfredo
RA   Berlingieri, Maria T.
RA   Di Fiore, Pier Paolo
RA   Portella, Giuseppe
RA   Grieco, Michele
RA   Vecchio, Giancarlo
RT   "Retraction for Fusco et al., 'One- and two-step transformations of rat
RT   thyroid epithelial cells by retroviral oncogenes'.";
RL   Mol. Cell. Biol. 38:e00663.17.1-e00663.17.1(2018).
//
RX   Patent=US4608341;
RA   Ambesi-Impiombato, Fransesco Saverio
RT   "Living, fast-growing thyroid cell strain, FRTL-5.";
RL   Patent number US4608341, 26-Aug-1986.
//
RX   PubMed=7836899; DOI=10.1677/joe.0.1430527;
RA   MacNeil, Sheila
RA   Munro, Donald S.
RA   Metcalfe, Russell Anthony
RA   Cotterell, S.
RA   Ruban, Ludmila N.
RA   Davies, Rose
RA   Weetman, Anthony Peter
RT   "An investigation of the ability of TSH and Graves' immunoglobulin G
RT   to increase intracellular calcium in human thyroid cells, rat FRTL-5
RT   thyroid cells and eukaryotic cells transfected with the human TSH
RT   receptor.";
RL   J. Endocrinol. 143:527-540(1994).
//
RX   PubMed=9709917; DOI=10.1089/thy.1998.8.623;
RA   Tasevski, Vitomir
RA   Benn, Diana
RA   Peters, Greg
RA   Luttrell, Brian
RA   Simpson, Ann
RT   "The Fischer rat thyroid cell line FRTL-5 exhibits a nondiploid
RT   karyotype.";
RL   Thyroid 8:623-626(1998).
//