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Cellosaurus C2C12 (CVCL_0188)

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Cell line name C2C12
Synonyms C2c12; C2-C12; C12
Accession CVCL_0188
Resource Identification Initiative To cite this cell line use: C2C12 (RRID:CVCL_0188)
Comments Part of: ENCODE project mouse cell lines.
Characteristics: Capable, upon starvation, of differentiating into contractile myotubes that express muscle-specific proteins.
Doubling time: 19.6 +- 0.4 hours (Note=At 22th passage), 18.4 +- 0.2 hours (Note=At 38th passage) (PubMed=22020321); ~20 hours (DSMZ=ACC-565).
Omics: Genomics; ChIP-seq; H3K27me3.
Omics: Genomics; ChIP-seq; H3K36me3.
Omics: Genomics; ChIP-seq; H3K4me3.
Omics: Genomics; ChIP-seq; H3K79me2.
Omics: Genomics; ChIP-seq; H3K79me3.
Omics: Metabolomics.
Omics: Proteomics.
Omics: Proteomics; PTM; Glycoproteome.
Omics: Proteomics; Subcellular; Cell surface.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Misspelling: C1C12; Note=Occasionally.
Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.
Cell type: Myoblast; CL=CL_0000056.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H.
Hierarchy Parent: CVCL_6812 (C2 [Mouse myoblast])
Children:
CVCL_AS32 (C2C12 Clone 5 DYSF shRNA)CVCL_UR38 (C2C12 Scrambled)CVCL_RQ02 (C2C12-Bmpr1b)
CVCL_B0JA (C2C12-BRE)CVCL_C8WR (C2C12-EGFP)CVCL_A0NQ (C2C12-hITGA11)
CVCL_C8UW (C2C12-Luc)CVCL_RQ03 (C2C12-R486Q-Bmpr1b)CVCL_RQ04 (C2C12-R486W-Bmpr1b)
CVCL_C9FH (C2C12/Cas9-hyg)CVCL_KS37 (C2C12/NFkB-luc)CVCL_E7J0 (C2C12(Cas9))
CVCL_VR09 (C2C12i)CVCL_0189 (C2C12N)CVCL_A6JR (ChR2-C2C12)
CVCL_3900 (COM-3)CVCL_E1JM (HyCyte C2C12 KO-mNqo1)CVCL_E1JN (HyCyte C2C12 KO-mNqo2)
CVCL_3902 (INC-2)CVCL_KW18 (PathHunter C2C12 CXCR4 beta-arrestin)CVCL_KW19 (PathHunter C2C12 CXCR5 beta-arrestin)
CVCL_KW20 (PathHunter C2C12 KISS1R beta-arrestin)CVCL_KW21 (PathHunter C2C12 TACR1 beta-arrestin)CVCL_D8F2 (Ubigene C2C12 Lmod2 KO)
CVCL_D8F3 (Ubigene C2C12 Micu1 KO)CVCL_D8F4 (Ubigene C2C12 Pdk4 KO)CVCL_D8F5 (Ubigene C2C12 Recql5 KO)
CVCL_D8F6 (Ubigene C2C12 Shmt2 KO)CVCL_D8F7 (Ubigene C2C12 Slc38a9 KO)CVCL_D8F8 (Ubigene C2C12 Trib1 KO)
CVCL_D8F9 (Ubigene C2C12 Vav3 KO)CVCL_D8FA (Ubigene C2C12 Zbtb5 KO)
Sex of cell Female
Age at sampling 2M
Category Spontaneously immortalized cell line
STR profile Source(s): Ubigene=YC-A010

Markers:
Mouse STR 4-219.3
Mouse STR 5-515
Mouse STR 6-418
Mouse STR 6-712
Mouse STR 12-116
Mouse STR 15-325.3
Mouse STR 18-316
Mouse STR X-125,26

Run an STR similarity search on this cell line
Web pages Info; -; -; https://web.archive.org/web/20130502024425/http://www.stanford.edu/group/blau/protocols/c2lineprotocol.html
Info; ECACC; -; https://www.culturecollections.org.uk/products/cell-cultures/ecacc-cell-line-profiles/c2c12/
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-123.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/C2C12
Provider; BRICS; BRICS0089; http://www.brics.ac.cn/cell/template/cell/cell_detail.html?id=3902
Publications

PubMed=563524; DOI=10.1038/270725a0
David Yaffe, Ora Saxel;
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
Nature 270:725-727(1977)

PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795
Debra Kay McMahon, Page Albert Willis Anderson, Rashid Nassar, J. Blair Bunting, Ziad Saba, Annette E. Oakeley, Nadia N. Malouf;
C2C12 cells: biophysical, biochemical, and immunocytochemical properties.
Am. J. Physiol. 266:C1795-C1802(1994)

PubMed=15596414
Sabrina Burattini, Paola Ferri, Michela Battistelli, Rosa Curci, Francesca Luchetti, Elisabetta Falcieri;
C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.
Eur. J. Histochem. 48:223-233(2004)

PubMed=19298647; DOI=10.1186/1471-2121-10-20; PMCID=PMC2663541
Blanca Herrera, Gareth J. Inman;
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
BMC Cell Biol. 10:20.1-20.11(2009)

PubMed=19350625; DOI=10.1002/bit.22318
Hideaki Fujita, Kazunori Shimizu, Eiji Nagamori;
Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.
Biotechnol. Bioeng. 103:1034-1041(2009)

PubMed=19656770; DOI=10.1074/mcp.M900195-MCP200; PMCID=PMC2773721
Rebekah L. Gundry, Kimberly Raginski, Yelena S. Tarasova, Irina Tchernyshyov, Damaris Bausch-Fluck, Steven T. Elliott, Kenneth R. Boheler, Jennifer Eileen Van Eyk, Bernd Wollscheid;
The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation.
Mol. Cell. Proteomics 8:2555-2569(2009)

PubMed=20635352; DOI=10.1002/bit.22865
Hideaki Fujita, Akiko Endo, Kazunori Shimizu, Eiji Nagamori;
Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
Biotechnol. Bioeng. 107:894-901(2010)

PubMed=22020321; DOI=10.1016/j.yexcr.2011.10.004; PMCID=PMC3541016
Glen W. Humphrey, Elena Mekhedov, Paul S. Blank, Antoine de Morree, Gulcin Pekkurnaz, Kanneboyina Nagaraju, Joshua Zimmerberg;
GREG cells, a dysferlin-deficient myogenic mouse cell line.
Exp. Cell Res. 318:127-135(2012)

PubMed=24735950; DOI=10.1016/j.scr.2014.03.004
Lena Willkomm, Sarah Schubert, Raphael Jung, Manuela Elsen, Julika Borde, Sebastian Gehlert, Frank Suhr, Wilhelm Bloch;
Lactate regulates myogenesis in C2C12 myoblasts in vitro.
Stem Cell Res. 12:742-753(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
John Paul Didion, Ryan J. Buus, Zohreh Naghashfar, David W. Threadgill, Herbert Carpenter Morse 3rd, Fernando Pardo-Manuel de Villena;
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314; PMCID=PMC4404347
Damaris Bausch-Fluck, Andreas Hofmann, Thomas Bock, Andreas P. Frei, Ferdinando Cerciello, Andrea Jacobs, Hansjoerg Moest, Ulrich Omasits, Rebekah L. Gundry, Charles Yoon, Ralph Schiess, Alexander Schmidt, Paulina Mirkowska ...Show all 20 authors... , Anetta Svitorka Hartlova, Jennifer Eileen Van Eyk, Jean-Pierre Bourquin, Rudolf Aebersold, Kenneth R. Boheler, Peter W. Zandstra, Bernd Wollscheid; Show fewer authors
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:e0121314.1-e0121314.22(2015)

Cross-references
Cell line collections (Providers) Abcam; ab279976
AddexBio; P0028001/4665
ATCC; CRL-1772
BCRC; 60083
BCRJ; 0058
CCTCC; GDC0175
CLS; 400476
DSMZ; ACC-565
ECACC; 91031101
IZSLER; BS CL 148
KCB; KCB 2012115YJ
NCBI_Iran; C521
RCB; RCB0987
Ubigene; YC-A010
Cell line databases/resources CLO; CLO_0002071
CLO; CLO_0050871
MCCL; MCC:0000079
CLDB; cl563
CLDB; cl564
CLDB; cl5172
CCRID; 1101MOU-PUMC000099
CCRID; 3101MOUGNM26
CCRID; 3101MOUSCSP505
CCRID; 4201MOU-CCTCC00175
DSMZCellDive; ACC-565
FCS-free; 221-17-457-3-4-3
FCS-free; 221-17-505-3-4-12
FCS-free; 221-21-488-3-4-12
FCS-free; 221-5-486-3-3
FCS-free; 221-5-527-1-3-3
Lonza; 692
TOKU-E; 728
Anatomy/cell type resources BTO; BTO_0000165
Biological sample resources ENCODE; ENCBS124ENC
ENCODE; ENCBS299ZOZ
ENCODE; ENCBS981MXJ
CRISP screens repositories BioGRID_ORCS_Cell_line; 1564
Chemistry resources ChEMBL-Cells; CHEMBL3308003
ChEMBL-Targets; CHEMBL612655
PubChem_Cell_line; CVCL_0188
Encyclopedic resources Wikidata; Q5008075
Experimental variables resources EFO; EFO_0001098
Gene expression databases GEO; GSE30192
GEO; GSM915162
GEO; GSM915166
GEO; GSM915174
GEO; GSM915179
GEO; GSM915182
GEO; GSM915186
GEO; GSM915188
GEO; GSM918408
GEO; GSM918410
GEO; GSM918411
GEO; GSM918415
GEO; GSM918417
GEO; GSM918422
GEO; GSM1354755
Metabolomic databases MetaboLights; MTBLS127
Proteomic databases PRIDE; PXD000022
PRIDE; PXD000288
PRIDE; PXD000328
PRIDE; PXD000589
PRIDE; PXD003211
PRIDE; PXD004151
PRIDE; PXD004960
PRIDE; PXD012986
PRIDE; PXD017465
PRIDE; PXD050867
PRIDE; PXD050903
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number39