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Cellosaurus C2C12 (CVCL_0188)

[Text version]
Cell line name C2C12
Synonyms C2c12; C2-C12; C12
Accession CVCL_0188
Resource Identification Initiative To cite this cell line use: C2C12 (RRID:CVCL_0188)
Comments Part of: ENCODE project mouse cell lines.
Characteristics: Capable, upon starvation, of differentiating into contractile myotubes that express muscle-specific proteins.
Doubling time: 19.6 +- 0.4 hours (Note=At 22th passage), 18.4 +- 0.2 (Note=At 38th passage) (PubMed=22020321); ~20 hours (DSMZ=ACC-565).
Omics: Cell surface proteome.
Omics: Deep proteome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K79me2 ChIP-seq epigenome analysis.
Omics: H3K79me3 ChIP-seq epigenome analysis.
Omics: Metabolome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Misspelling: C1C12; Note=Occasionally.
Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.
Cell type: Myoblast; CL=CL_0000056.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H.
Hierarchy Parent: CVCL_6812 (C2 [Mouse myoblast])
CVCL_AS32 (C2C12 Clone 5 DYSF shRNA)CVCL_UR38 (C2C12 Scrambled)CVCL_RQ02 (C2C12-Bmpr1b)
CVCL_C8UW (C2C12-Luc)CVCL_RQ03 (C2C12-R486Q-Bmpr1b)CVCL_RQ04 (C2C12-R486W-Bmpr1b)
CVCL_C9FH (C2C12/Cas9-hyg)CVCL_KS37 (C2C12/NFkB-luc)CVCL_VR09 (C2C12i)
CVCL_0189 (C2C12N)CVCL_A6JR (ChR2-C2C12)CVCL_3900 (COM-3)
CVCL_3902 (INC-2)CVCL_KW18 (PathHunter C2C12 CXCR4 beta-arrestin)CVCL_KW19 (PathHunter C2C12 CXCR5 beta-arrestin)
CVCL_KW20 (PathHunter C2C12 KISS1R beta-arrestin)CVCL_KW21 (PathHunter C2C12 TACR1 beta-arrestin)
Sex of cell Female
Age at sampling 2M
Category Spontaneously immortalized cell line
STR profile Source(s): Ubigene

Mouse STR 4-219.3
Mouse STR 5-515
Mouse STR 6-418
Mouse STR 6-712
Mouse STR 12-116
Mouse STR 15-325.3
Mouse STR 18-316
Mouse STR X-125,26

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/C2C12

PubMed=563524; DOI=10.1038/270725a0
Yaffe D., Saxel O.
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
Nature 270:725-727(1977)

PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795
McMahon D.K., Anderson P.A.W., Nassar R., Bunting J.B., Saba Z., Oakeley A.E., Malouf N.N.
C2C12 cells: biophysical, biochemical, and immunocytochemical properties.
Am. J. Physiol. 266:C1795-C1802(1994)

Burattini S., Ferri P., Battistelli M., Curci R., Luchetti F., Falcieri E.
C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.
Eur. J. Histochem. 48:223-233(2004)

PubMed=19298647; DOI=10.1186/1471-2121-10-20
Herrera B., Inman G.J.
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
BMC Cell Biol. 10:20.1-20.11(2009)

PubMed=19350625; DOI=10.1002/bit.22318
Fujita H., Shimizu K., Nagamori E.
Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.
Biotechnol. Bioeng. 103:1034-1041(2009)

PubMed=20635352; DOI=10.1002/bit.22865
Fujita H., Endo A., Shimizu K., Nagamori E.
Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
Biotechnol. Bioeng. 107:894-901(2010)

PubMed=22020321; DOI=10.1016/j.yexcr.2011.10.004
Humphrey G.W., Mekhedov E., Blank P.S., de Morree A., Pekkurnaz G., Nagaraju K., Zimmerberg J.
GREG cells, a dysferlin-deficient myogenic mouse cell line.
Exp. Cell Res. 318:127-135(2012)

PubMed=24735950; DOI=10.1016/j.scr.2014.03.004
Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S., Suhr F., Bloch W.
Lactate regulates myogenesis in C2C12 myoblasts in vitro.
Stem Cell Res. 12:742-753(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

Cell line collections (Providers) Abcam; ab279976
AddexBio; P0028001/4665
ATCC; CRL-1772
BCRC; 60083
BCRJ; 0058
CLS; 400476
ECACC; 91031101
KCB; KCB 2012115YJ
NCBI_Iran; C521
RCB; RCB0987
Ubigene; YC-A010
Cell line databases/resources CLO; CLO_0002071
CLO; CLO_0050871
MCCL; MCC:0000079
CLDB; cl563
CLDB; cl564
CLDB; cl5172
CCRID; 1101MOU-PUMC000099
DSMZCellDive; ACC-565
FCS-free; 221-5-486-3-3
FCS-free; 221-5-527-1-3-3
FCS-free; 221-17-457-3-4-3
FCS-free; 221-17-505-3-4-12
FCS-free; 221-21-488-3-4-12
Lonza; 692
TOKU-E; 728
Anatomy/cell type resources BTO; BTO:0000165
Biological sample resources ENCODE; ENCBS124ENC
CRISP screens repositories BioGRID_ORCS_Cell_line; 1564
Chemistry resources ChEMBL-Cells; CHEMBL3308003
ChEMBL-Targets; CHEMBL612655
PubChem_Cell_line; CVCL_0188
Encyclopedic resources Wikidata; Q5008075
Experimental variables resources EFO; EFO_0001098
Gene expression databases GEO; GSE30192
GEO; GSM915162
GEO; GSM915166
GEO; GSM915174
GEO; GSM915179
GEO; GSM915182
GEO; GSM915186
GEO; GSM915188
GEO; GSM918408
GEO; GSM918410
GEO; GSM918411
GEO; GSM918415
GEO; GSM918417
GEO; GSM918422
GEO; GSM1354755
Metabolomic databases MetaboLights; MTBLS127
Proteomic databases PRIDE; PXD000022
PRIDE; PXD000288
PRIDE; PXD000328
PRIDE; PXD000589
PRIDE; PXD003211
PRIDE; PXD004151
PRIDE; PXD004960
PRIDE; PXD012986
PRIDE; PXD017465
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number36