ID   C2C12
AC   CVCL_0188
SY   C2c12; C2-C12; C12
DR   BTO; BTO:0000165
DR   CLO; CLO_0002071
DR   CLO; CLO_0050871
DR   EFO; EFO_0001098
DR   MCCL; MCC:0000079
DR   CLDB; cl563
DR   CLDB; cl564
DR   CLDB; cl5172
DR   Abcam; ab279976
DR   AddexBio; P0028001/4665
DR   ATCC; CRL-1772
DR   BCRC; 60083
DR   BCRJ; 0058
DR   CCRID; 1101MOU-PUMC000099
DR   CCRID; 4201MOU-CCTCC00175
DR   ChEMBL-Cells; CHEMBL3308003
DR   ChEMBL-Targets; CHEMBL612655
DR   CLS; 400476
DR   DSMZ; ACC-565
DR   DSMZCellDive; ACC-565
DR   ECACC; 91031101
DR   FCS-free; 221-5-486-3-3
DR   FCS-free; 221-5-527-1-3-3
DR   FCS-free; 221-17-457-3-4-3
DR   FCS-free; 221-17-505-3-4-12
DR   FCS-free; 221-21-488-3-4-12
DR   GEO; GSE30192
DR   GEO; GSM915162
DR   GEO; GSM915166
DR   GEO; GSM915174
DR   GEO; GSM915179
DR   GEO; GSM915182
DR   GEO; GSM915186
DR   GEO; GSM915188
DR   GEO; GSM918408
DR   GEO; GSM918410
DR   GEO; GSM918411
DR   GEO; GSM918415
DR   GEO; GSM918417
DR   GEO; GSM918422
DR   GEO; GSM1354755
DR   KCB; KCB 2012115YJ
DR   Lonza; 692
DR   MetaboLights; MTBLS127
DR   NCBI_Iran; C521
DR   PRIDE; PXD000022
DR   PRIDE; PXD000288
DR   PRIDE; PXD000328
DR   PRIDE; PXD000589
DR   PRIDE; PXD003211
DR   PRIDE; PXD004151
DR   PRIDE; PXD004960
DR   PRIDE; PXD012986
DR   PRIDE; PXD017465
DR   PubChem_Cell_line; CVCL_0188
DR   RCB; RCB0987
DR   TOKU-E; 728
DR   Ubigene; YC-A010
DR   Wikidata; Q5008075
RX   PubMed=563524;
RX   PubMed=8023908;
RX   PubMed=15596414;
RX   PubMed=19298647;
RX   PubMed=19350625;
RX   PubMed=20635352;
RX   PubMed=22020321;
RX   PubMed=24735950;
RX   PubMed=25277546;
RX   PubMed=25894527;
CC   Part of: ENCODE project mouse cell lines.
CC   Characteristics: Capable, upon starvation, of differentiating into contractile myotubes that express muscle-specific proteins.
CC   Doubling time: 19.6 +- 0.4 hours (Note=At 22th passage), 18.4 +- 0.2 (Note=At 38th passage) (PubMed=22020321); ~20 hours (DSMZ=ACC-565).
CC   Omics: Cell surface proteome.
CC   Omics: Deep proteome analysis.
CC   Omics: H3K27me3 ChIP-seq epigenome analysis.
CC   Omics: H3K36me3 ChIP-seq epigenome analysis.
CC   Omics: H3K4me3 ChIP-seq epigenome analysis.
CC   Omics: H3K79me2 ChIP-seq epigenome analysis.
CC   Omics: H3K79me3 ChIP-seq epigenome analysis.
CC   Omics: Metabolome analysis.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Misspelling: C1C12; Note=Occasionally.
CC   Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.
CC   Cell type: Myoblast; CL=CL_0000056.
CC   Breed/subspecies: C3H.
ST   Source(s): Ubigene
ST   Mouse STR 12-1: 16
ST   Mouse STR 15-3: 25.3
ST   Mouse STR 18-3: 16
ST   Mouse STR 4-2: 19.3
ST   Mouse STR 5-5: 15
ST   Mouse STR 6-4: 18
ST   Mouse STR 6-7: 12
ST   Mouse STR X-1: 25,26
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_6812 ! C2 [Mouse myoblast]
SX   Female
AG   2M
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 35
RX   PubMed=563524; DOI=10.1038/270725a0;
RA   Yaffe D., Saxel O.;
RT   "Serial passaging and differentiation of myogenic cells isolated from
RT   dystrophic mouse muscle.";
RL   Nature 270:725-727(1977).
RX   PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795;
RA   McMahon D.K., Anderson P.A.W., Nassar R., Bunting J.B., Saba Z.,
RA   Oakeley A.E., Malouf N.N.;
RT   "C2C12 cells: biophysical, biochemical, and immunocytochemical
RT   properties.";
RL   Am. J. Physiol. 266:C1795-C1802(1994).
RX   PubMed=15596414;
RA   Burattini S., Ferri P., Battistelli M., Curci R., Luchetti F.,
RA   Falcieri E.;
RT   "C2C12 murine myoblasts as a model of skeletal muscle development:
RT   morpho-functional characterization.";
RL   Eur. J. Histochem. 48:223-233(2004).
RX   PubMed=19298647; DOI=10.1186/1471-2121-10-20;
RA   Herrera B., Inman G.J.;
RT   "A rapid and sensitive bioassay for the simultaneous measurement of
RT   multiple bone morphogenetic proteins. Identification and
RT   quantification of BMP4, BMP6 and BMP9 in bovine and human serum.";
RL   BMC Cell Biol. 10:20.1-20.11(2009).
RX   PubMed=19350625; DOI=10.1002/bit.22318;
RA   Fujita H., Shimizu K., Nagamori E.;
RT   "Novel method for fabrication of skeletal muscle construct from the
RT   C2C12 myoblast cell line using serum-free medium AIM-V.";
RL   Biotechnol. Bioeng. 103:1034-1041(2009).
RX   PubMed=20635352; DOI=10.1002/bit.22865;
RA   Fujita H., Endo A., Shimizu K., Nagamori E.;
RT   "Evaluation of serum-free differentiation conditions for C2C12
RT   myoblast cells assessed as to active tension generation capability.";
RL   Biotechnol. Bioeng. 107:894-901(2010).
RX   PubMed=22020321; DOI=10.1016/j.yexcr.2011.10.004;
RA   Humphrey G.W., Mekhedov E., Blank P.S., de Morree A., Pekkurnaz G.,
RA   Nagaraju K., Zimmerberg J.;
RT   "GREG cells, a dysferlin-deficient myogenic mouse cell line.";
RL   Exp. Cell Res. 318:127-135(2012).
RX   PubMed=24735950; DOI=10.1016/j.scr.2014.03.004;
RA   Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S.,
RA   Suhr F., Bloch W.;
RT   "Lactate regulates myogenesis in C2C12 myoblasts in vitro.";
RL   Stem Cell Res. 12:742-753(2014).
RX   PubMed=25277546; DOI=10.1186/1471-2164-15-847;
RA   Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W.,
RA   Morse H.C. III, Pardo-Manuel de Villena F.;
RT   "SNP array profiling of mouse cell lines identifies their strains of
RT   origin and reveals cross-contamination and widespread aneuploidy.";
RL   BMC Genomics 15:847.1-847.11(2014).
RX   PubMed=25894527; DOI=10.1371/journal.pone.0121314;
RA   Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F.,
RA   Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R.,
RA   Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P.,
RA   Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.;
RT   "A mass spectrometric-derived cell surface protein atlas.";
RL   PLoS ONE 10:E0121314-E0121314(2015).