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Cellosaurus HC-04 (CVCL_Z631)

[Text version]
Cell line name HC-04
Synonyms HC04; HCO4
Accession CVCL_Z631
Resource Identification Initiative To cite this cell line use: HC-04 (RRID:CVCL_Z631)
Comments Group: Malaria research cell line.
Group: Patented cell line.
Problematic cell line: Possibly contaminated. Indicated (PubMed=24532842) to have a near genetic identity with Hep-G2 cells. But they are functionally different from Hep-G2 cells since they support sporozoite invasion and liver stage exoerythrocytic development of Plasmodium falciparum and Plasmodium vivax. But STR profile from BEI Resources is identical to that of Hep-G2. Originally thought to originate from the spontaneous immortalization of a normal liver tissue of an hepatoma patient.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-3441.
Population: Caucasian.
Doubling time: ~24 hours (PubMed=17590308).
Omics: Deep proteome analysis.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0027 (Hep-G2)
CVCL_B7D7 (HC-04.J7)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
STR profile Source(s): BEI_resources


Run an STR similarity search on this cell line

Prachumsri J., Yimamnuaychok N.
Human liver cell line.
Patent number US7015036, 21-Mar-2006

PubMed=16687667; DOI=10.4269/ajtmh.2006.74.708
Sattabongkot J., Yimamnuaychok N., Leelaudomlipi S., Rasameesoraj M., Jenwithisuk R., Coleman R.E., Udomsangpetch R., Cui L.-W., Brewer T.G.
Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax.
Am. J. Trop. Med. Hyg. 74:708-715(2006)

PubMed=17590308; DOI=10.1016/j.tiv.2007.05.003
Lim P.L.K., Tan W.-Q., Latchoumycandane C., Mok W.C., Khoo Y.M., Lee H.S., Sattabongkot J., Beerheide W., Lim S.G., Tan T.M.C., Boelsterli U.A.
Molecular and functional characterization of drug-metabolizing enzymes and transporter expression in the novel spontaneously immortalized human hepatocyte line HC-04.
Toxicol. In Vitro 21:1390-1401(2007)

PubMed=22990793; DOI=10.1007/978-1-62703-026-7_27
Sinnis P., De La Vega P., Coppi A., Krzych U., Mota M.M.
Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry.
Methods Mol. Biol. 923:385-400(2013)

PubMed=24532842; DOI=10.1074/mcp.M113.035584
Tao D.-Y., King J.G., Tweedell R.E., Jost P.J., Boddey J.A., Dinglasan R.R.
The acute transcriptomic and proteomic response of HC-04 hepatoma cells to hepatocyte growth factor and its implications for Plasmodium falciparum sporozoite invasion.
Mol. Cell. Proteomics 13:1153-1164(2014)

PubMed=30891005; DOI=10.3389/fmicb.2019.00127
Tweedell R.E., Tao D.-Y., Hamerly T., Robinson T.M., Larsen S., Gronning A.G.B., Norris A.M., King J.G., Law H.C.-H., Baumbach J., Bergmann-Leitner E.S., Dinglasan R.R.
The selection of a hepatocyte cell line susceptible to Plasmodium falciparum sporozoite invasion that is associated with expression of glypican-3.
Front. Microbiol. 10:127.1-127.16(2019)

Cell line collections (Providers) BEI_Resources; MRA-975
Cell line databases/resources cancercelllines; CVCL_Z631
Encyclopedic resources Wikidata; Q54881462
Gene expression databases ArrayExpress; E-MTAB-6919
Proteomic databases PRIDE; PXD008613
Entry history
Entry creation10-Apr-2015
Last entry update30-Jan-2024
Version number22