Cellosaurus logo
expasy logo

Cellosaurus HC-04 (CVCL_Z631)

[Text version]
Cell line name HC-04
Synonyms HC04; HCO4
Accession CVCL_Z631
Resource Identification Initiative To cite this cell line use: HC-04 (RRID:CVCL_Z631)
Comments Group: Malaria research cell line.
Group: Patented cell line.
Problematic cell line: Possibly contaminated. Indicated (PubMed=24532842) to have a near genetic identity with Hep-G2 cells. But they are functionally different from Hep-G2 cells since they support sporozoite invasion and liver stage exoerythrocytic development of Plasmodium falciparum and Plasmodium vivax. But STR profile from BEI Resources is identical to that of Hep-G2. Originally thought to originate from the spontaneous immortalization of a normal liver tissue of an hepatoma patient.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-3441.
Population: Caucasian.
Doubling time: ~24 hours (PubMed=17590308).
Omics: Proteomics.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Hepatoblastoma (NCIt: C3728)
Hepatoblastoma (ORDO: Orphanet_449)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0027 (Hep-G2)
Children:
CVCL_B7D7 (HC-04.J7)
Sex of cell Male
Age at sampling 15Y
Category Cancer cell line
STR profile Source(s): BEI_Resources=MRA-975

Markers:
AmelogeninX,Y
CSF1PO10,11
D5S81811,12
D7S82010
D13S3179,13
D16S53912
TH019
TPOX8,9
vWA17

Run an STR similarity search on this cell line
Publications

Patent=US7015036
Jetsumon Prachumsri, Nongnuch Yimamnuaychok;
Human liver cell line.
Patent number US7015036, 21-Mar-2006

PubMed=16687667; DOI=10.4269/ajtmh.2006.74.708
Jetsumon Sattabongkot, Nongnuch Yimamnuaychok, Surasak Leelaudomlipi, Maneerat Rasameesoraj, Rachaneeporn Jenwithisuk, Russell E. Coleman, Rachanee Udomsangpetch, Li-Wang Cui, Thomas G. Brewer;
Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax.
Am. J. Trop. Med. Hyg. 74:708-715(2006)

PubMed=17590308; DOI=10.1016/j.tiv.2007.05.003
Priscilla L.K. Lim, Wei-Qi Tan, Calivarathan Latchoumycandane, Wei Chuen Mok, Yok Moi Khoo, How Sung Lee, Jetsumon Sattabongkot, Walter Beerheide, Seng Gee Lim, Theresa M.C. Tan, Urs Alex Boelsterli;
Molecular and functional characterization of drug-metabolizing enzymes and transporter expression in the novel spontaneously immortalized human hepatocyte line HC-04.
Toxicol. In Vitro 21:1390-1401(2007)

PubMed=22990793; DOI=10.1007/978-1-62703-026-7_27; PMCID=PMC3951895
Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, Maria Manuel Mota;
Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry.
Methods Mol. Biol. 923:385-400(2013)

PubMed=24532842; DOI=10.1074/mcp.M113.035584; PMCID=PMC4014276
Ding-Yin Tao, Jonas G. King, Rebecca E. Tweedell, Philipp J. Jost, Justin A. Boddey, Rhoel R. Dinglasan;
The acute transcriptomic and proteomic response of HC-04 hepatoma cells to hepatocyte growth factor and its implications for Plasmodium falciparum sporozoite invasion.
Mol. Cell. Proteomics 13:1153-1164(2014)

PubMed=30891005; DOI=10.3389/fmicb.2019.00127; PMCID=PMC6413710
Rebecca E. Tweedell, Ding-Yin Tao, Timothy Hamerly, Tanisha M. Robinson, Simon Larsen, Alexander G.B. Gronning, Alessandra M. Norris, Jonas G. King, Henry Chun-Hin Law, Jan Baumbach, Elke S. Bergmann-Leitner, Rhoel R. Dinglasan;
The selection of a hepatocyte cell line susceptible to Plasmodium falciparum sporozoite invasion that is associated with expression of glypican-3.
Front. Microbiol. 10:127.1-127.16(2019)

Cross-references
Cell line collections (Providers) BEI_Resources; MRA-975
Cell line databases/resources cancercelllines; CVCL_Z631
Encyclopedic resources Wikidata; Q54881462
Gene expression databases ArrayExpress; E-MTAB-6919
Proteomic databases PRIDE; PXD008613
Entry history
Entry creation10-Apr-2015
Last entry update10-Apr-2025
Version number25