ID   HC-04
AC   CVCL_Z631
SY   HC04; HCO4
DR   ArrayExpress; E-MTAB-6919
DR   BEI_Resources; MRA-975
DR   cancercelllines; CVCL_Z631
DR   PRIDE; PXD008613
DR   Wikidata; Q54881462
RX   Patent=US7015036;
RX   PubMed=16687667;
RX   PubMed=17590308;
RX   PubMed=22990793;
RX   PubMed=24532842;
RX   PubMed=30891005;
CC   Group: Malaria research cell line.
CC   Group: Patented cell line.
CC   Problematic cell line: Possibly contaminated. Indicated (PubMed=24532842) to have a near genetic identity with Hep-G2 cells. But they are functionally different from Hep-G2 cells since they support sporozoite invasion and liver stage exoerythrocytic development of Plasmodium falciparum and Plasmodium vivax. But STR profile from BEI Resources is identical to that of Hep-G2. Originally thought to originate from the spontaneous immortalization of a normal liver tissue of an hepatoma patient.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-3441.
CC   Population: Caucasian.
CC   Doubling time: ~24 hours (PubMed=17590308).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Omics: Proteomics.
CC   Omics: Transcriptomics; RNAseq.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
ST   Source(s): BEI_Resources=MRA-975
ST   Amelogenin: X,Y
ST   CSF1PO: 10,11
ST   D13S317: 9,13
ST   D16S539: 12
ST   D5S818: 11,12
ST   D7S820: 10
ST   TH01: 9
ST   TPOX: 8,9
ST   vWA: 17
DI   NCIt; C3728; Hepatoblastoma
DI   ORDO; Orphanet_449; Hepatoblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0027 ! Hep-G2
SX   Male
AG   15Y
CA   Cancer cell line
DT   Created: 10-04-15; Last updated: 10-04-25; Version: 25
//
RX   Patent=US7015036;
RA   Prachumsri J., Yimamnuaychok N.;
RT   "Human liver cell line.";
RL   Patent number US7015036, 21-Mar-2006.
//
RX   PubMed=16687667; DOI=10.4269/ajtmh.2006.74.708;
RA   Sattabongkot J., Yimamnuaychok N., Leelaudomlipi S., Rasameesoraj M.,
RA   Jenwithisuk R., Coleman R.E., Udomsangpetch R., Cui L.-W.,
RA   Brewer T.G.;
RT   "Establishment of a human hepatocyte line that supports in vitro
RT   development of the exo-erythrocytic stages of the malaria parasites
RT   Plasmodium falciparum and P. vivax.";
RL   Am. J. Trop. Med. Hyg. 74:708-715(2006).
//
RX   PubMed=17590308; DOI=10.1016/j.tiv.2007.05.003;
RA   Lim P.L.K., Tan W.-Q., Latchoumycandane C., Mok W.C., Khoo Y.M.,
RA   Lee H.S., Sattabongkot J., Beerheide W., Lim S.G., Tan T.M.C.,
RA   Boelsterli U.A.;
RT   "Molecular and functional characterization of drug-metabolizing
RT   enzymes and transporter expression in the novel spontaneously
RT   immortalized human hepatocyte line HC-04.";
RL   Toxicol. In Vitro 21:1390-1401(2007).
//
RX   PubMed=22990793; DOI=10.1007/978-1-62703-026-7_27; PMCID=PMC3951895;
RA   Sinnis P., De La Vega P., Coppi A., Krzych U., Mota M.M.;
RT   "Quantification of sporozoite invasion, migration, and development by
RT   microscopy and flow cytometry.";
RL   Methods Mol. Biol. 923:385-400(2013).
//
RX   PubMed=24532842; DOI=10.1074/mcp.M113.035584; PMCID=PMC4014276;
RA   Tao D.-Y., King J.G., Tweedell R.E., Jost P.J., Boddey J.A.,
RA   Dinglasan R.R.;
RT   "The acute transcriptomic and proteomic response of HC-04 hepatoma
RT   cells to hepatocyte growth factor and its implications for Plasmodium
RT   falciparum sporozoite invasion.";
RL   Mol. Cell. Proteomics 13:1153-1164(2014).
//
RX   PubMed=30891005; DOI=10.3389/fmicb.2019.00127; PMCID=PMC6413710;
RA   Tweedell R.E., Tao D.-Y., Hamerly T., Robinson T.M., Larsen S.,
RA   Gronning A.G.B., Norris A.M., King J.G., Law H.C.-H., Baumbach J.,
RA   Bergmann-Leitner E.S., Dinglasan R.R.;
RT   "The selection of a hepatocyte cell line susceptible to Plasmodium
RT   falciparum sporozoite invasion that is associated with expression of
RT   glypican-3.";
RL   Front. Microbiol. 10:127.1-127.16(2019).
//