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Cellosaurus HBEC3-KT (CVCL_X491)

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Cell line name HBEC3-KT
Synonyms HBEC3 KT; HBEC 3KT; HBEC3KT; HBEC3 Cdk4-hTERT; HBEC3
Accession CVCL_X491
Resource Identification Initiative To cite this cell line use: HBEC3-KT (RRID:CVCL_X491)
Comments Part of: Human Protein Atlas, Cell Atlas panel.
Doubling time: 36-48 hours (Evercyte=CkHT-004-0230).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transduction; Gene=MGI; MGI:88357; Cdk4.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; Expression; Large antibody panel staining analysis.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
Cell type: Epithelial cell of bronchus; CL=CL_0002328.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_EQ78 (HBEC3KT53)
Originate from same individual CVCL_D4C2 ! HBEC3-ET
Sex of cell Female
Age at sampling 65Y
Category Telomerase immortalized cell line
STR profile Source(s): ATCC=CRL-4051

Markers:
AmelogeninX
CSF1PO10,11
D5S81812
D7S8208,12
D13S31712,13
D16S5399,12
TH019.3
TPOX11,12
vWA15,17

Run an STR similarity search on this cell line
Web pages Info; HPA; -; https://www.proteinatlas.org/learn/cellines
Publications

PubMed=15604268; DOI=10.1158/0008-5472.CAN-04-3703
Ruben D. Ramirez, Shelley Sheridan, Luc Girard, Mitsuo Sato, Young Kim, Jon Pollack, Michael Peyton, Ying Zou, Jonathan M. Kurie, J. Michael DiMaio, Sara Milchgrub ...Show all 21 authors... , Alice L. Smith, Rhonda F. Souza, Laura Gilbey, Xi Zhang, Kenia G. Gandia, Melville B. Vaughan, Woodring Erik Wright, Adi F. Gazdar, Jerry W. Shay, John D. Minna; Show fewer authors
Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins.
Cancer Res. 64:9027-9034(2004)

PubMed=20079948; DOI=10.1016/j.lungcan.2009.12.005; PMCID=PMC3110769
Adi F. Gazdar, Bin Gao, John D. Minna;
Lung cancer cell lines: useless artifacts or invaluable tools for medical science?
Lung Cancer 68:309-318(2010)

PubMed=20624269; DOI=10.1186/1756-9966-29-75; PMCID=PMC2907339
Li-Qin Du, Jeoffrey J. Schageman, Irnov Irnov, Luc Girard, Scott M. Hammond, John D. Minna, Adi F. Gazdar, Alexander Pertsemlidis;
MicroRNA expression distinguishes SCLC from NSCLC lung tumor cells and suggests a possible pathological relationship between SCLCs and NSCLCs.
J. Exp. Clin. Cancer Res. 29:75.1-75.12(2010)

PubMed=21760947; DOI=10.1371/journal.pone.0022023; PMCID=PMC3131301
Oliver Delgado, Aadil A. Kaisani, Monica Spinola, Xian-Jin Xie, Kimberly G. Batten, John D. Minna, Woodring Erik Wright, Jerry W. Shay;
Multipotent capacity of immortalized human bronchial epithelial cells.
PLoS ONE 6:e22023.1-e22023.8(2011)

PubMed=24240690; DOI=10.1038/onc.2013.486; PMCID=PMC4025984
Elma Zaganjor, Jihan K. Osborne, Lauren M. Weil, Laura A. Diaz-Martinez, Joshua X. Gonzales, Stina M. Singel, Jill E. Larsen, Luc Girard, John D. Minna, Melanie H. Cobb;
Ras regulates kinesin 13 family members to control cell migration pathways in transformed human bronchial epithelial cells.
Oncogene 33:5457-5466(2014)

PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540
Elizabeth A. McMillan, Myung-Jeom Ryu, Caroline H. Diep, Saurabh Mendiratta, Jean Rene Clemenceau, Rachel M. Vaden, Ju-Hwa Kim, Takashi Motoyaji, Kyle R. Covington, Michael Peyton, Kenneth Huffman ...Show all 40 authors... , Xiao-Feng Wu, Luc Girard, Yeojin Sung, Pei-Hsaun Chen, Prema Latha Mallipeddi, Joo Young Lee, Jordan Hanson, Sukesh Voruganti, Yunku Yu, Sunho Park, Jessica Sudderth, Christopher DeSevo, Donna M. Muzny, HarshaVardhan Doddapaneni, Adi F. Gazdar, Richard Alexander Gibbs, Tae Hyun Hwang, John Victor Heymach, Ignacio Ivan Wistuba, Kevin Robert Coombes, Noelle S. Williams, David A. Wheeler, John B. MacMillan, Ralph J. DeBerardinis, Michael G. Roth, Bruce Arthur Posner, John D. Minna, Hyun Seok Kim, Michael A. White; Show fewer authors
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)

PubMed=32586780; DOI=10.1016/j.resinv.2020.04.005
Mitsuo Sato, Jerry W. Shay, John D. Minna;
Immortalized normal human lung epithelial cell models for studying lung cancer biology.
Respir. Investig. 58:344-354(2020)

Cross-references
Cell line collections (Providers) ABM; T0753
ATCC; CRL-4051
Evercyte; CkHT-004-0230
Encyclopedic resources Wikidata; Q54881400
Gene expression databases GEO; GSM171895
GEO; GSM253438
GEO; GSM794409
GEO; GSM7701486
GEO; GSM7701487
Entry history
Entry creation05-Sep-2014
Last entry update14-Aug-2025
Version number26