ID   HBEC3-KT
AC   CVCL_X491
SY   HBEC3 KT; HBEC 3KT; HBEC3KT; HBEC3 Cdk4-hTERT; HBEC3
DR   ABM; T0753
DR   ATCC; CRL-4051
DR   Evercyte; CkHT-004-0230
DR   GEO; GSM171895
DR   GEO; GSM253438
DR   GEO; GSM794409
DR   GEO; GSM7701486
DR   GEO; GSM7701487
DR   Wikidata; Q54881400
RX   PubMed=15604268;
RX   PubMed=20079948;
RX   PubMed=20624269;
RX   PubMed=21760947;
RX   PubMed=24240690;
RX   PubMed=29681454;
RX   PubMed=32586780;
WW   Info; HPA; -; https://www.proteinatlas.org/learn/cellines
CC   Part of: Human Protein Atlas, Cell Atlas panel.
CC   Doubling time: 36-48 hours (Evercyte=CkHT-004-0230).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:88357; Cdk4.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Omics: Genomics; Whole exome sequencing.
CC   Omics: Proteomics; Expression; Large antibody panel staining analysis.
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Transcriptomics; miRNA profiling; Microarray.
CC   Omics: Transcriptomics; RNAseq.
CC   Omics: Variations; SNP array analysis.
CC   Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
CC   Cell type: Epithelial cell of bronchus; CL=CL_0002328.
ST   Source(s): ATCC=CRL-4051
ST   Amelogenin: X
ST   CSF1PO: 10,11
ST   D13S317: 12,13
ST   D16S539: 9,12
ST   D5S818: 12
ST   D7S820: 8,12
ST   TH01: 9.3
ST   TPOX: 11,12
ST   vWA: 15,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
OI   CVCL_D4C2 ! HBEC3-ET
SX   Female
AG   65Y
CA   Telomerase immortalized cell line
DT   Created: 05-09-14; Last updated: 10-04-25; Version: 25
//
RX   PubMed=15604268; DOI=10.1158/0008-5472.CAN-04-3703;
RA   Ramirez R.D., Sheridan S., Girard L., Sato M., Kim Y., Pollack J.,
RA   Peyton M., Zou Y., Kurie J.M., DiMaio J.M., Milchgrub S., Smith A.L.,
RA   Souza R.F., Gilbey L., Zhang X., Gandia K.G., Vaughan M.B., Wright W.E.,
RA   Gazdar A.F., Shay J.W., Minna J.D.;
RT   "Immortalization of human bronchial epithelial cells in the absence of
RT   viral oncoproteins.";
RL   Cancer Res. 64:9027-9034(2004).
//
RX   PubMed=20079948; DOI=10.1016/j.lungcan.2009.12.005; PMCID=PMC3110769;
RA   Gazdar A.F., Gao B., Minna J.D.;
RT   "Lung cancer cell lines: useless artifacts or invaluable tools for
RT   medical science?";
RL   Lung Cancer 68:309-318(2010).
//
RX   PubMed=20624269; DOI=10.1186/1756-9966-29-75; PMCID=PMC2907339;
RA   Du L.-Q., Schageman J.J., Irnov I., Girard L., Hammond S.M., Minna J.D.,
RA   Gazdar A.F., Pertsemlidis A.;
RT   "MicroRNA expression distinguishes SCLC from NSCLC lung tumor cells
RT   and suggests a possible pathological relationship between SCLCs and
RT   NSCLCs.";
RL   J. Exp. Clin. Cancer Res. 29:75.1-75.12(2010).
//
RX   PubMed=21760947; DOI=10.1371/journal.pone.0022023; PMCID=PMC3131301;
RA   Delgado O., Kaisani A.A., Spinola M., Xie X.-J., Batten K.G.,
RA   Minna J.D., Wright W.E., Shay J.W.;
RT   "Multipotent capacity of immortalized human bronchial epithelial
RT   cells.";
RL   PLoS ONE 6:e22023.1-e22023.8(2011).
//
RX   PubMed=24240690; DOI=10.1038/onc.2013.486; PMCID=PMC4025984;
RA   Zaganjor E., Osborne J.K., Weil L.M., Diaz-Martinez L.A.,
RA   Gonzales J.X., Singel S.M., Larsen J.E., Girard L., Minna J.D.,
RA   Cobb M.H.;
RT   "Ras regulates kinesin 13 family members to control cell migration
RT   pathways in transformed human bronchial epithelial cells.";
RL   Oncogene 33:5457-5466(2014).
//
RX   PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540;
RA   McMillan E.A., Ryu M.-J., Diep C.H., Mendiratta S., Clemenceau J.R.,
RA   Vaden R.M., Kim J.-H., Motoyaji T., Covington K.R., Peyton M.,
RA   Huffman K., Wu X.-F., Girard L., Sung Y., Chen P.-H., Mallipeddi P.L.,
RA   Lee J.Y., Hanson J., Voruganti S., Yu Y., Park S., Sudderth J.,
RA   DeSevo C., Muzny D.M., Doddapaneni H., Gazdar A.F., Gibbs R.A.,
RA   Hwang T.H., Heymach J.V., Wistuba I.I., Coombes K.R., Williams N.S.,
RA   Wheeler D.A., MacMillan J.B., DeBerardinis R.J., Roth M.G.,
RA   Posner B.A., Minna J.D., Kim H.S., White M.A.;
RT   "Chemistry-first approach for nomination of personalized treatment in
RT   lung cancer.";
RL   Cell 173:864-878.e29(2018).
//
RX   PubMed=32586780; DOI=10.1016/j.resinv.2020.04.005;
RA   Sato M., Shay J.W., Minna J.D.;
RT   "Immortalized normal human lung epithelial cell models for studying
RT   lung cancer biology.";
RL   Respir. Investig. 58:344-354(2020).
//