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Cellosaurus CRISPRi Gen1C 5 (CVCL_VM38)

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Cell line name CRISPRi Gen1C 5
Synonyms WTC CRISPRi Gen1 clone 5; CRISPRi (Gen1C 5); 1C-5
Accession CVCL_VM38
Resource Identification Initiative To cite this cell line use: CRISPRi Gen1C 5 (RRID:CVCL_VM38)
Comments Population: Japanese.
Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Children:
CVCL_VQ27 (WTC CRISPRi HOPX g1)CVCL_VQ28 (WTC CRISPRi HOPX g4)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=26971820; DOI=10.1016/j.stem.2016.01.022; PMCID=PMC4830697
Mohammad A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Annie Truong, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristin Holmes, C. Ian Spencer, Luke M. Judge ...Show all 22 authors... , David E. Gordon, Tilde Veng Eskildsen, Jacqueline E. Villalta, Max A. Horlbeck, Luke A. Gilbert, Nevan J. Krogan, Soren P. Sheikh, Jonathan S. Weissman, Lei S. Qi, Po-Lin So, Bruce R. Conklin; Show fewer authors
CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.
Cell Stem Cell 18:541-553(2016)

PubMed=30290179; DOI=10.1016/j.stem.2018.09.009; PMCID=PMC6220122
Clayton E. Friedman, Quan Nguyen, Samuel W. Lukowski, Abbigail Helfer, Han Sheng Chiu, Jason Miklas, Shiri Levy, Sheng-Bao Suo, Jing-Dong Jackie Han, Pierre Osteil, Guang-Dun Peng ...Show all 26 authors... , Nai-He Jing, Gregory J. Baillie, Anne Senabouth, Angelika N. Christ, Timothy James Caird Bruxner, Charles E. Murry, Emily S. Wong, Jun Ding, Yu-Liang Wang, James Hudson, Hannele Ruohola-Baker, Ziv Bar-Joseph, Patrick Ping Leu Tam, Joseph E. Powell, Nathan J. Palpant; Show fewer authors
Single-cell transcriptomic analysis of cardiac differentiation from human PSCs reveals HOPX-dependent cardiomyocyte maturation.
Cell Stem Cell 23:586-598.e8(2018)

Cross-references
Encyclopedic resources Wikidata; Q93458763
Entry history
Entry creation07-Sep-2018
Last entry update14-Aug-2025
Version number13