Cellosaurus WTC CRISPRi HOPX g4 (CVCL_VQ28)
| Cell line name | WTC CRISPRi HOPX g4 |
|---|---|
| Accession | CVCL_VQ28 |
| Resource Identification Initiative | To cite this cell line use: WTC CRISPRi HOPX g4 (RRID:CVCL_VQ28) |
| Comments | Population: Japanese. Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi). Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry was introduced in the AAVS1 safe harbor locus. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:24961; HOPX. Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511. Cell type: Fibroblast of skin; CL=CL_0002620. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_VM38 (CRISPRi Gen1C 5) |
| Sex of cell | Male |
| Age at sampling | 30Y |
| Category | Induced pluripotent stem cell |
| Publications | PubMed=30290179; DOI=10.1016/j.stem.2018.09.009; PMCID=PMC6220122 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q98135034 |
| Entry history | |
| Entry creation | 07-Sep-2018 |
| Last entry update | 14-Aug-2025 |
| Version number | 15 |