Cellosaurus logo
expasy logo

Cellosaurus WTC CRISPRi HOPX g4 (CVCL_VQ28)

[Text version]
Cell line name WTC CRISPRi HOPX g4
Accession CVCL_VQ28
Resource Identification Initiative To cite this cell line use: WTC CRISPRi HOPX g4 (RRID:CVCL_VQ28)
Comments Population: Japanese.
Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry was introduced in the AAVS1 safe harbor locus.
Knockout cell: Method=CRISPR/Cas9; HGNC; 24961; HOPX.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_VM38 (CRISPRi Gen1C 5)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

DOI=10.1101/229294
Friedman C.E., Nguyen Q., Lukowski S.W., Chiu H.S., Helfer A., Miklas J., Suo S.-B., Han J.-D.J., Osteil P., Peng G.-D., Jing N.-H., Baillie G.J., Senabouth A., Christ A.N., Bruxner T.J.C., Murry C.E., Wong E.S., Ding J., Wang Y.-L., Hudson J., Ruohola-Baker H., Bar-Joseph Z., Tam P.P.L., Powell J.E., Palpant N.J.
Analysis of cardiac differentiation at single cell resolution reveals a requirement of hypertrophic signaling for HOPX transcription.
bioRxiv 2017:229294-229294(2017)

Cross-references
Encyclopedic resources Wikidata; Q98135034
Entry history
Entry creation07-Sep-2018
Last entry update30-Jan-2024
Version number11